LOCUS all_or_nothing 4276 bp DNA linear 07-May-2024 ACCESSION all_or_nothing VERSION all_or_nothing.1 FEATURES Location/Qualifiers promoter 9..1995 /label=XylSPm misc_feature 1996..2005 /label=RBS_cassette misc_feature 2006..2016 /label=Spacer CDS 2017..3096 /label=LacI misc_feature 3097..3133 /label=Coupling CDS 2006..3133 /label=LacI_cassette misc_feature 3134..3136 /label=ATG misc_feature 3137..3142 /label=NheI CDS 3143..3856 /label=msfGFP misc_feature 3857..3862 /label=EcoRI CDS 3134..3862 /label=gfp_cassette misc_feature 3863..3921 /label=Restriction_sites primer_bind 3922..3945 /label=PBS_F24 misc_feature 3863..3945 /label=MCS terminator 3946..3975 /label=T500 misc_feature 9..3975 /label=all_or_nothing_Pm promoter complement(243..295) /label=PA1_04s misc_feature complement(220..242) /label=sRNA_target misc_feature complement(135..219) /label=MICc_scaffold CDS complement(135..242) /label=sRNA_cassette terminator complement(1..134) /label=T1_TE misc_feature complement(3976..4270) /label=all_or_nothing_PA1_04s misc_feature 4271..4276 /label=SpeI misc_feature 1..8 /label=PacI ORIGIN 1 ttaattaaac gttcgtaatc aagccacttc ctttttgcat tgacgcaggg tgtcggaagg 61 caactcgccg aacgcgctcc tatagttttc agcgaagcgt cccaaatgta agaagccgta 121 gtctagggct atctcagtta tactacgcac attggcactg ggatcgttca agcaggcgcg 181 gatgctttcg agcttgcggt tgcggatgta gttcttcggc gtggtgccgg cgtgcttctc 241 gaacaaattg tagagcgagc gtggactcat catcgccagc tccgctaacc gctcaaggct 301 gatattccgt ttgagattct cctcaatgaa ttgaacgact cgctcgaaag acgggttacc 361 tttgctgaaa atttcacggc tgacattgct gcccagcatt tcgagcagct tggaagcgat 421 gatccccgca tagtgctctt ggacccgagg catcgacttt gtatgttccg cttcgtcaca 481 aactaacccg agtagattga taaagccatc gagttgctgg agattgtgtc gcgcggcgaa 541 acggataccc tccctcggct tgtgccaatt gttgtcactg cacgcccgat caaggaccac 601 tgagggcaat ttaacgataa atttctcgca atcttctgaa taggtcaggt cggcttggtc 661 atccggattg agcagcaata gttcgcccgg cgcaaaatag tgctcctggc catggccacg 721 ccacaggcaa tggcctttga gtattatttg cagatgataa caggtctcta atccaggcga 781 gattaccctc acgctaccgc cgtagctgat tcgacacaga tcgaggcatc cgaagattct 841 gtggtgcagc ctgcctgccg ggcgcccgcc cttgggcagg cgaatagagt gcgtaccgac 901 atactggtta acataatcgg agactgcata gggctcggcg tggacgaaga tctgactttt 961 ctcgttcaat aagcaaaaat ccatagttca cggttctctt attttaatgt gggctgcttg 1021 gtgtgatgta gaaaggcgcc aagtcgatga aaatgcatct cgacgtgatg cgtatacggg 1081 ttacccccat tgccacgttg cgccatcctt tttgcaatca gtgaccactt ttccaagcaa 1141 aaataacgcc aagcagaacg aagacgttct ttttaagaag cgagaacacc agaagttcgt 1201 gctgtcgggg catggggcga cgaattggcg gataaagggg atctgctgga tattacggcc 1261 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt 1321 gcccgcctga tgaatgctca tccgtaatta cgtatggcaa tgaaagacgg tgagctggtg 1381 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca 1441 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat 1501 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt 1561 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg 1621 gacaacttct tcgcccccgt tttcaccatg catgggaatt agcttgatct gaccaacgac 1681 cggtagcgga gctatccaac ggcggtatac caggaaaaca cacagcaggt acatcagaac 1741 agtaccatga ctgaagaaca aatagttttt tcctgatcca taaagcagaa cggcctgctc 1801 catgacaaat ctggctcccc aactaatgcc ccatgcagcc agcataacca gcataaagtg 1861 cagtgtccgg tttgataggg ataagtccag ccttgcaaga agcggataca ggagtgcaaa 1921 aaatggctat ctctagtaag gcctacccct taggctttat gcaacagaaa caataataat 1981 ggagtcatga ccatgcctag gaggaaaaaa catatggtga aaccagtaac gttatacgat 2041 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc 2101 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt 2161 cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc 2221 tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat 2281 caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa 2341 gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg 2401 gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt 2461 gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga 2521 ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca 2581 ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat 2641 caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa 2701 accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag 2761 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc 2821 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc 2881 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct 2941 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc 3001 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 3061 ctggcacgac aggtttcccg actggaaagc gggcagcatc atcaccacca tcattaggat 3121 ggtggtgatg ataatggcta gccgtaaagg tgaagaactg ttcaccggtg ttgttccgat 3181 cctggttgaa ctggatggtg atgttaacgg ccacaaattc tctgttcgtg gtgaaggtga 3241 aggtgatgca accaacggta aactgaccct gaaattcatc tgcactaccg gtaaactgcc 3301 ggttccatgg ccgactctgg tgactaccct gacctatggt gttcagtgtt tttctcgtta 3361 cccggatcac atgaagcagc atgatttctt caaatctgca atgccggaag gttatgtaca 3421 ggagcgcacc atttctttca aagacgatgg cacctacaaa acccgtgcag aggttaaatt 3481 tgaaggtgat actctggtga accgtattga actgaaaggc attgatttca aagaggacgg 3541 caacatcctg ggccacaaac tggaatataa cttcaactcc cataacgttt acatcaccgc 3601 agacaaacag aagaacggta tcaaagctaa cttcaaaatt cgccataacg ttgaagacgg 3661 tagcgtacag ctggcggacc actaccagca gaacactccg atcggtgatg gtccggttct 3721 gctgccggat aaccactacc tgtccaccca gtctaaactg tccaaagacc cgaacgaaaa 3781 gcgcgaccac atggtgctgc tggagttcgt tactgccgca ggtatcacgc acggcatgga 3841 tgaactctac aaataagaat tcgagctcgg tacccgggga tcctctagag tcgacctgca 3901 ggcatgcaag cttgcggccg cgtcgtgact gggaaaaccc tggcgcaaag cccgccgaaa 3961 ggcgggcttt tctgttataa acgcagaaag gcccacccga aggtgagcca gtgtgactct 4021 agtagagagc gttcaccgac aaaaacagat aaaacgaaag gcccagtctt tcgactgagc 4081 ctttcgtttt atttgatgcc tggctcgagc tcgagaaaaa aagcccggac gactgttcgg 4141 gcttgtcttt ttatatgttg gaaaatcagt ggcaatgcaa tggcccaaca gaaaatggtg 4201 aaaccagtaa cgttatactg aatctaagta tcaatgtgag cgctcacaag tcaacactct 4261 ttttgataaa actagt //