LOCUS IBMc071 5110 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T ACCESSION IBMc071 VERSION IBMc071.1 FEATURES Location/Qualifiers RBS 2714..2724 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="BBa_B0033" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" terminator 3321..3373 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" misc_feature 3374..3394 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" rep_origin 3955..4779 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" CDS 2731..3309 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="ECF20" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" primer_bind complement(3489..3508) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcAct tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagtcacaca ggactactag atgaatgaaa ccgatcctga tctggaactg 2761 ctgaaacgta ttggtaataa tgatgcacag gccgttaaag aaatggttac ccgtaaactg 2821 cctcgtctgc tggcactggc aagtcgcctg ctgggtgatg cagatgaagc acgtgatatt 2881 gcacaagaaa gttttctgcg catttggaaa caggcagcaa gctggcgtag cgaacaggca 2941 cgttttgata cctggctgca tcgtgttgca ctgaatctgt gttatgatcg tctgcgtcgt 3001 cgtaaagaac atgtgccggt tgatagcgaa catgcctgtg aagcactgga tacccgtccg 3061 gcaccggatg aacagctgga agcaagcgca cagagccgtc gtatggcaca ggcactggat 3121 cagctgccgg atcgtcagcg tgaagcaatt gttctgcagt attatcaaga actgagcaat 3181 accgaagcag cagcactgat gcaaattagc gttgaagccc tggaaagcct gctgagccgt 3241 gcacgtcgta atctgcgtag ccatctggcc gaagcaccgg gtgcagatct gagcggtcgt 3301 cgcaaaccgt aatactagag aacgcatgag AAAGCCCCCG GAAGATCACC TTCCGGGGGC 3361 TTTtttattg cgctactagt agcggccgct gcaggagtca ctaagggtta gttagttaga 3421 ttagcagaaa gtcaaaagcc tccgaccgga ggcttttgac taaaacttcc cttggggtta 3481 tcattggggc tcactcaaag gcggtaatca gataaaaaaa atccttagct ttcgctaagg 3541 atgatttctg ctagagatgg aatagactgg atggaggcgg ataaagttgc aggaccactt 3601 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt 3661 ggatctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt 3721 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 3781 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 3841 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 3901 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccttaata 3961 agatgatctt cttgagatcg ttttggtctg cgcgtaatct cttgctctga aaacgaaaaa 4021 accgccttgc agggcggttt ttcgaaggtt ctctgagcta ccaactcttt gaaccgaggt 4081 aactggcttg gaggagcgca gtcaccaaaa cttgtccttt cagtttagcc ttaaccggcg 4141 catgacttca agactaactc ctctaaatca attaccagtg gctgctgcca gtggtgcttt 4201 tgcatgtctt tccgggttgg actcaagacg atagttaccg gataaggcgc agcggtcgga 4261 ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgcctacc cggaactgag 4321 tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa 4381 aggcaggaac aggagagcgc acgagggagc cgccagggga aacgcctggt atctttatag 4441 tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg 4501 gcggagccta tggaaaaacg gctttgccgc ggccctctca cttccctgtt aagtatcttc 4561 ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga 4621 acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatcctgta tcacatattc 4681 tgctgacgca ccggtgcagc cttttttctc ctgccacatg aagcacttca ctgacaccct 4741 catcagtgcc aacatagtaa gccagtatac actccgctag cgctgaggtc tgcctcgtga 4801 agaaggtgtt gctgactcat accaggcctg aatcgcccca tcatccagcc agaaagtgag 4861 ggagccacgg ttgatgagag ctttgttgta ggtggaccag ttggtgattt tgaacttttg 4921 ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg atctgatcct tcaactcagc 4981 aaaagttcga tttattcaac aaagccacgt tgtgtctcaa aatctctgat gttacattgc 5041 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac 5101 aaggggtgtt //