LOCUS IBMc078 4705 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-27)-BsaI-BsaI-mCherry*(218 -236)-T ACCESSION IBMc078 VERSION IBMc078.1 FEATURES Location/Qualifiers promoter 125..213 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" terminator 3032..3084 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" CDS complement(1632..2510) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" misc_feature 3085..3105 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" rep_origin 3666..4490 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" misc_feature 1610..1631 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" CDS 2963..3020 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*" RBS 2830..2844 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" primer_bind 1487..1506 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" CDS 2854..2932 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*" primer_bind complement(3200..3219) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" misc_feature 2940..2955 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="spacing sequence" CDS 214..1407 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" promoter 2538..2821 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" ORIGIN 1 ttcaacaaag ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata 61 tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgtttact 121 agaggagatt ctcatgtttg acagcttatc atcgataagc tttaatgcgg tagtttatca 181 cagttaaatt gctaacgcag tcaggcaccg tgtatgaaat ctaacaatgc gctcatcgtc 241 attctcggca ccgtcaccct ggacgctgta ggcataggct tggttatgcc ggtactgccg 301 ggcctcttgc gggatatcgt ccattccgac agtattgcca gtcactatgg cgtgctgctt 361 gcgctctatg cgttgatgca atttctttgc gcacccgttc tcggagccct gtccgaccgc 421 tttggccgcc gtccagtcct gctcgcttcg ctccttggag ccactatcga ctacgcgatc 481 atggcgacca cacccgtcct gtggattctc tacgccggac gcatcgtggc gggcatcacg 541 ggtgccacag gtgcggttgc tggtgcctat atcgccgaca tcaccgatgg ggaagatcgg 601 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg 661 gccgggggac tgttgggtgc catctccttg catgcaccat tccttgcggc ggcggtgctc 721 aacggcctca acctcctcct gggctgcttc cttatgcagg aatcgcataa gggagagcgc 781 cgtccgatgc ccttgcgtgc cttcaatcca gtcagctcct tccggtgggc gcggggcatg 841 actatcgtcg ccgcacttat gactgttttc tttatcatgc aactcgtagg acaggttccg 901 gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc 961 ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt cacgggcccc 1021 gccaccaaac gtttcggcga gaagcaggcc attatcgcgg gcatggcggc cgacgcgctg 1081 ggctacgtct tgctggcgtt cgcgacgcgc ggctggatgg ccttccccat tatgattctt 1141 ctcgcttccg gcggcatcgg tatgcccgcg ttgcaggcca tgctgtcccg ccaagtagat 1201 gacgaccatc agggacagct tcaagggtcg ctcgcggctc ttaccagcct cacttcgatc 1261 attggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg 1321 gcatggattg taggtgccgc cctttacctt gtctgcctcc ccgcgttgcg tcgcggtgca 1381 tggagccggg ccacctcgac ctaataatac tagctccggc aaaaaaacgg gcaaggtgtc 1441 accaccctgc cctttttctt taaaaccgaa aagattactt cgcgtttgcc acctgacgtc 1501 taagaaaagg aatattcagc aatttgcccg tgccgaagaa aggcccaccc gtgaaggtga 1561 gccagtgagt tgattgctac gtaattagtt agttagccct tagtgactcg aattcgcggc 1621 cgcttctaga gttatgacaa cttgacggct acatcattca ctttttcttc acaaccggca 1681 cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga 1741 tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc 1801 agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc 1861 tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg 1921 atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacccga 1981 ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc 2041 tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg 2101 atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt catccgggcg aaagaacccc 2161 gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag 2221 taaacccact ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg atgaatctct 2281 cctggcggga acagcaaaat atcacccggt cggcaaacaa attctcgtcc ctgatttttc 2341 accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg 2401 tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg 2461 gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata 2521 ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc 2581 gtcttttact ggcActtctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg 2641 taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc 2701 agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta 2761 tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca 2821 ttactagaga ttaaagagga gaaatactag atggtgagca agggcgaaga aGATaacatg 2881 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaTGAGACCa 2941 cggccacatc gtggaGGTCT CAacagtacg aacgcgccga gggccgccac tccaccggcg 3001 gcatggacga gctgtacaag taatactaga gaacgcatga gAAAGCCCCC GGAAGATCAC 3061 CTTCCGGGGG CTTTtttatt gcgctactag tagcggccgc tgcaggagtc actaagggtt 3121 agttagttag attagcagaa agtcaaaagc ctccgaccgg aggcttttga ctaaaacttc 3181 ccttggggtt atcattgggg ctcactcaaa ggcggtaatc agataaaaaa aatccttagc 3241 tttcgctaag gatgatttct gctagagatg gaatagactg gatggaggcg gataaagttg 3301 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag 3361 ccggtgagcg tggatctcgc ggtatcattg cagcactggg gccagatggt aagccctccc 3421 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga 3481 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat 3541 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 3601 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 3661 accccttaat aagatgatct tcttgagatc gttttggtct gcgcgtaatc tcttgctctg 3721 aaaacgaaaa aaccgccttg cagggcggtt tttcgaaggt tctctgagct accaactctt 3781 tgaaccgagg taactggctt ggaggagcgc agtcaccaaa acttgtcctt tcagtttagc 3841 cttaaccggc gcatgacttc aagactaact cctctaaatc aattaccagt ggctgctgcc 3901 agtggtgctt ttgcatgtct ttccgggttg gactcaagac gatagttacc ggataaggcg 3961 cagcggtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgcctac 4021 ccggaactga gtgtcaggcg tggaatgaga caaacgcggc cataacagcg gaatgacacc 4081 ggtaaaccga aaggcaggaa caggagagcg cacgagggag ccgccagggg aaacgcctgg 4141 tatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcagat ttcgtgatgc 4201 ttgtcagggg ggcggagcct atggaaaaac ggctttgccg cggccctctc acttccctgt 4261 taagtatctt cctggcatct tccaggaaat ctccgccccg ttcgtaagcc atttccgctc 4321 gccgcagtcg aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa tatatcctgt 4381 atcacatatt ctgctgacgc accggtgcag ccttttttct cctgccacat gaagcacttc 4441 actgacaccc tcatcagtgc caacatagta agccagtata cactccgcta gcgctgaggt 4501 ctgcctcgtg aagaaggtgt tgctgactca taccaggcct gaatcgcccc atcatccagc 4561 cagaaagtga gggagccacg gttgatgaga gctttgttgt aggtggacca gttggtgatt 4621 ttgaactttt gctttgccac ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc 4681 ttcaactcag caaaagttcg attta //