LOCUS IBMc176 5184 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-6)-BsaI-BsaI-M86 (149-154)-S-ECF20(102-193)-T ACCESSION IBMc176 VERSION IBMc176.1 FEATURES Location/Qualifiers primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" terminator 3395..3447 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" CDS 3037..3055 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86N" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" rep_origin 4029..4853 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" CDS 3108..3383 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="ECF20 102-193" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" CDS 2731..3033 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="ECF20 1-101" misc_feature 3448..3468 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" CDS 3086..3104 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86C" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" misc_feature 3063..3078 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="spacing sequence" RBS 2714..2724 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="BBa_B0033" primer_bind complement(3563..3582) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcAct tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagtcacaca ggactactag atgaatgaaa ccgatcctga tctggaactg 2761 ctgaaacgta ttggtaataa tgatgcacag gccgttaaag aaatggttac ccgtaaactg 2821 cctcgtctgc tggcactggc aagtcgcctg ctgggtgatg cagatgaagc acgtgatatt 2881 gcacaagaaa gttttctgcg catttggaaa caggcagcaa gctggcgtag cgaacaggca 2941 cgttttgata cctggctgca tcgtgttgca ctgaatctgt gttatgatcg tctgcgtcgt 3001 cgtaaagaac atgtgccggt tgatagcgaa catGGAtgca tctcgggaga tagttTGAGA 3061 CCacggccac atcgtggaGG TCTCAtgaca tcattgtaca taacTCAgcc tgtgaagcac 3121 tggatacccg tccggcaccg gatgaacagc tggaagcaag cgcacagagc cgtcgtatgg 3181 cacaggcact ggatcagctg ccggatcgtc agcgtgaagc aattgttctg cagtattatc 3241 aagaactgag caataccgaa gcagcagcac tgatgcaaat tagcgttgaa gccctggaaa 3301 gcctgctgag ccgtgcacgt cgtaatctgc gtagccatct ggccgaagca ccgggtgcag 3361 atctgagcgg tcgtcgcaaa ccgtaatact agagaacgca tgagAAAGCC CCCGGAAGAT 3421 CACCTTCCGG GGGCTTTttt attgcgctac tagtagcggc cgctgcagga gtcactaagg 3481 gttagttagt tagattagca gaaagtcaaa agcctccgac cggaggcttt tgactaaaac 3541 ttcccttggg gttatcattg gggctcactc aaaggcggta atcagataaa aaaaatcctt 3601 agctttcgct aaggatgatt tctgctagag atggaataga ctggatggag gcggataaag 3661 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 3721 gagccggtga gcgtggatct cgcggtatca ttgcagcact ggggccagat ggtaagccct 3781 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 3841 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 3901 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 3961 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 4021 cagacccctt aataagatga tcttcttgag atcgttttgg tctgcgcgta atctcttgct 4081 ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga gctaccaact 4141 ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc ctttcagttt 4201 agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc agtggctgct 4261 gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt accggataag 4321 gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc 4381 tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca gcggaatgac 4441 accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag gggaaacgcc 4501 tggtatcttt atagtcctgt cgggtttcgc caccactgat ttgagcgtca gatttcgtga 4561 tgcttgtcag gggggcggag cctatggaaa aacggctttg ccgcggccct ctcacttccc 4621 tgttaagtat cttcctggca tcttccagga aatctccgcc ccgttcgtaa gccatttccg 4681 ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaatatatcc 4741 tgtatcacat attctgctga cgcaccggtg cagccttttt tctcctgcca catgaagcac 4801 ttcactgaca ccctcatcag tgccaacata gtaagccagt atacactccg ctagcgctga 4861 ggtctgcctc gtgaagaagg tgttgctgac tcataccagg cctgaatcgc cccatcatcc 4921 agccagaaag tgagggagcc acggttgatg agagctttgt tgtaggtgga ccagttggtg 4981 attttgaact tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga 5041 tccttcaact cagcaaaagt tcgatttatt caacaaagcc acgttgtgtc tcaaaatctc 5101 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac 5161 ataaacagta atacaagggg tgtt //