LOCUS IBMc227 4272 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR(1-20)-BsaI-BsaI-TetR(188-207)- Ter ACCESSION IBMc227 VERSION IBMc227.1 FEATURES Location/Qualifiers CDS 2575..2634 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="TetR(188-207)" RBS 2457..2484 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="tet1" primer_bind 1114..1133 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" misc_feature 2699..2719 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" CDS 174..1034 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="ampR (BsaI-)" misc_feature 2552..2567 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="spacing sequence" CDS 2485..2544 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="TetR(1-20)" CDS complement(1259..2137) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" terminator 2646..2698 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" primer_bind complement(2814..2833) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" rep_origin 3280..4104 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" misc_feature 1237..1258 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" promoter 2165..2448 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" ORIGIN 1 gggaagatgc gtgatctgat ccttcaactc agcaaaagtt cgatttattc aacaaagcca 61 cgttgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac 121 aataaaactg tctgcttaca taaacagtaa tacaaggggt gtttactaga ggtatgagta 181 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 241 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 301 gttatatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 361 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg 421 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 481 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 541 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 601 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 661 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 721 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 781 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 841 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtGGA tctcgcggta 901 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 961 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 1021 ttaagcattg gtaatactag ctccggcaaa aaaacgggca aggtgtcacc accctgccct 1081 ttttctttaa aaccgaaaag attacttcgc gtttgccacc tgacgtctaa gaaaaggaat 1141 attcagcaat ttgcccgtgc cgaagaaagg cccacccgtg aaggtgagcc agtgagttga 1201 ttgctacgta attagttagt tagcccttag tgactcgaat tcgcggccgc ttctagagtt 1261 atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg aactcgctcg 1321 ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa 1381 cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc ttcgcctggc 1441 tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg cggaaaagat 1501 gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata tcaaaattgc 1561 tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta tccatcggtg 1621 gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca agcagattta 1681 tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt tgcccaaaca 1741 ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata 1801 ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa acccactggt 1861 gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct ggcgggaaca 1921 gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc accccctgac 1981 cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg ataaaaaaat 2041 cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca ttaaacgagt 2101 atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc ccgccattca 2161 gagaagaaac caattgtcca tattgcatca gacattgccg tcactgcgtc ttttactggc 2221 Acttctcgct aaccaaaccg gtaaccccgc ttattaaaag cattctgtaa caaagcggga 2281 ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga aaagtccaca 2341 ttgattattt gcacggcgtc acactttgct atgccatagc atttttatcc ataagattag 2401 cggatcctac ctgacgcttt ttatcgcaac tctctactgt ttctccatta ctagagggaa 2461 gagagtcaat tcagggtggt gaatatgtcc agattagata aaagtaaagt gattaacagc 2521 gcattagagc tgcttaatga ggtcTGAGAC Cacggccaca tcgtggaGGT CTCAttcggc 2581 cttgaattga tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtcctgatac 2641 tagagaacgc atgagAAAGC CCCCGGAAGA TCACCTTCCG GGGGCTTTtt tattgcgcta 2701 ctagtagcgg ccgctgcagg agtcactaag ggttagttag ttagattagc agaaagtcaa 2761 aagcctccga ccggaggctt ttgactaaaa cttcccttgg ggttatcatt ggggctcact 2821 caaaggcggt aatcagataa aaaaaatcct tagctttcgc taaggatgat ttctgctaga 2881 gatggaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 2941 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtggatc tcgcggtatc 3001 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 3061 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 3121 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 3181 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 3241 ccttaacgtg agttttcgtt ccactgagcg tcagacccct taataagatg atcttcttga 3301 gatcgttttg gtctgcgcgt aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc 3361 ggtttttcga aggttctctg agctaccaac tctttgaacc gaggtaactg gcttggagga 3421 gcgcagtcac caaaacttgt cctttcagtt tagccttaac cggcgcatga cttcaagact 3481 aactcctcta aatcaattac cagtggctgc tgccagtggt gcttttgcat gtctttccgg 3541 gttggactca agacgatagt taccggataa ggcgcagcgg tcggactgaa cggggggttc 3601 gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat 3661 gagacaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag 3721 agcgcacgag ggagccgcca ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 3781 ccaccactga tttgagcgtc agatttcgtg atgcttgtca ggggggcgga gcctatggaa 3841 aaacggcttt gccgcggccc tctcacttcc ctgttaagta tcttcctggc atcttccagg 3901 aaatctccgc cccgttcgta agccatttcc gctcgccgca gtcgaacgac cgagcgtagc 3961 gagtcagtga gcgaggaagc ggaatatatc ctgtatcaca tattctgctg acgcaccggt 4021 gcagcctttt ttctcctgcc acatgaagca cttcactgac accctcatca gtgccaacat 4081 agtaagccag tatacactcc gctagcgctg aggtctgcct cgtgaagaag gtgttgctga 4141 ctcataccag gcctgaatcg ccccatcatc cagccagaaa gtgagggagc cacggttgat 4201 gagagctttg ttgtaggtgg accagttggt gattttgaac ttttgctttg ccacggaacg 4261 gtctgcgttg tc //