LOCUS IBMc326 4607 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-5)-BsaI-BsaI-ECF20(187-193)-T er ACCESSION IBMc326 VERSION IBMc326.1 FEATURES Location/Qualifiers terminator 2818..2870 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" CDS 2731..2747 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="ECF20" misc_feature 2871..2891 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" RBS 2714..2724 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="BBa_B0033" CDS 2785..2809 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="ECF20" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" rep_origin 3452..4276 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" primer_bind complement(2986..3005) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcAct tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagtcacaca ggactactag atgaatgaaa ccgatcctga gaccCTGTCT 2761 CTTATACACA TCTATTCggt ctcATctgag cggtcgtcgc aaaccgtaat actagagaac 2821 gcatgagAAA GCCCCCGGAA GATCACCTTC CGGGGGCTTT tttattgcgc tactagtagc 2881 ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc aaaagcctcc 2941 gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca ctcaaaggcg 3001 gtaatcagat aaaaaaaatc cttagctttc gctaaggatg atttctgcta gagatggaat 3061 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 3121 ctggtttatt gctgataaat ctggagccgg tgagcgtgga tctcgcggta tcattgcagc 3181 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 3241 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 3301 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 3361 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 3421 tgagttttcg ttccactgag cgtcagaccc cttaataaga tgatcttctt gagatcgttt 3481 tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg gcggtttttc 3541 gaaggttctc tgagctacca actctttgaa ccgaggtaac tggcttggag gagcgcagtc 3601 accaaaactt gtcctttcag tttagcctta accggcgcat gacttcaaga ctaactcctc 3661 taaatcaatt accagtggct gctgccagtg gtgcttttgc atgtctttcc gggttggact 3721 caagacgata gttaccggat aaggcgcagc ggtcggactg aacggggggt tcgtgcatac 3781 agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga atgagacaaa 3841 cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg 3901 agggagccgc caggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact 3961 gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggct 4021 ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca ggaaatctcc 4081 gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt 4141 gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg gtgcagcctt 4201 ttttctcctg ccacatgaag cacttcactg acaccctcat cagtgccaac atagtaagcc 4261 agtatacact ccgctagcgc tgaggtctgc ctcgtgaaga aggtgttgct gactcatacc 4321 aggcctgaat cgccccatca tccagccaga aagtgaggga gccacggttg atgagagctt 4381 tgttgtaggt ggaccagttg gtgattttga acttttgctt tgccacggaa cggtctgcgt 4441 tgtcgggaag atgcgtgatc tgatccttca actcagcaaa agttcgattt attcaacaaa 4501 gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat 4561 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgtt //