LOCUS IBMc329 5174 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B30-SrpR-Ter ACCESSION IBMc329 VERSION IBMc329.1 FEATURES Location/Qualifiers RBS 2714..2728 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" terminator 3385..3437 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" rep_origin 4019..4843 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" CDS 2735..3373 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="SrpR" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" misc_feature 3438..3458 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" primer_bind complement(3553..3572) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcAct tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagattaaag aggagaaata ctagATGGCA CGTAAAACCG CAGCAGAAGC 2761 AGAAGAAACC CGTCAGCGTA TTATTGATGC AGCACTGGAA GTTTTTGTTG CACAGGGTGT 2821 TAGTGATGCA ACCCTGGATC AGATTGCACG TAAAGCCGGT GTTACCCGTG GTGCAGTTTA 2881 TTGGCATTTT AATGGTAAAC TGGAAGTTCT GCAAGCAGTT CTGGCAAGCC GTCAGCATCC 2941 GCTGGAACTG GATTTTACAC CGGATCTGGG TATTGAACGT AGCTGGGAAG CAGTTGTTGT 3001 TGCAATGCTG GATGCAGTTC ATAGTCCGCA GAGCAAACAG TTTAGCGAAA TTCTGATTTA 3061 TCAGGGTCTG GATGAAAGCG GTCTGATTCA TAATCGTATG GTTCAGGCAA GCGATCGTTT 3121 TCTGCAATAT ATTCATCAGG TTCTGCGTCA TGCAGTTACC CAGGGTGAAC TGCCGATTAA 3181 TCTGGATCTG CAAACCAGCA TTGGTGTTTT TAAAGGTCTG ATTACCGGTC TGCTGTATGA 3241 AGGTCTGCGT AGCAAAGATC AGCAGGCACA GATTATCAAA GTTGCACTGG GTAGCTTTTG 3301 GGCACTGCTG CGTGAACCGC CTCGTTTTCT GCTGTGTGAA GAAGCACAGA TTAAACAGGT 3361 GAAATCCTTC GAATAAtact agagaacgca tgagAAAGCC CCCGGAAGAT CACCTTCCGG 3421 GGGCTTTttt attgcgctac tagtagcggc cgctgcagga gtcactaagg gttagttagt 3481 tagattagca gaaagtcaaa agcctccgac cggaggcttt tgactaaaac ttcccttggg 3541 gttatcattg gggctcactc aaaggcggta atcagataaa aaaaatcctt agctttcgct 3601 aaggatgatt tctgctagag atggaataga ctggatggag gcggataaag ttgcaggacc 3661 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 3721 gcgtggatct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 3781 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 3841 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 3901 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 3961 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagacccctt 4021 aataagatga tcttcttgag atcgttttgg tctgcgcgta atctcttgct ctgaaaacga 4081 aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga gctaccaact ctttgaaccg 4141 aggtaactgg cttggaggag cgcagtcacc aaaacttgtc ctttcagttt agccttaacc 4201 ggcgcatgac ttcaagacta actcctctaa atcaattacc agtggctgct gccagtggtg 4261 cttttgcatg tctttccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 4321 cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc tacccggaac 4381 tgagtgtcag gcgtggaatg agacaaacgc ggccataaca gcggaatgac accggtaaac 4441 cgaaaggcag gaacaggaga gcgcacgagg gagccgccag gggaaacgcc tggtatcttt 4501 atagtcctgt cgggtttcgc caccactgat ttgagcgtca gatttcgtga tgcttgtcag 4561 gggggcggag cctatggaaa aacggctttg ccgcggccct ctcacttccc tgttaagtat 4621 cttcctggca tcttccagga aatctccgcc ccgttcgtaa gccatttccg ctcgccgcag 4681 tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaatatatcc tgtatcacat 4741 attctgctga cgcaccggtg cagccttttt tctcctgcca catgaagcac ttcactgaca 4801 ccctcatcag tgccaacata gtaagccagt atacactccg ctagcgctga ggtctgcctc 4861 gtgaagaagg tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag 4921 tgagggagcc acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact 4981 tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact 5041 cagcaaaagt tcgatttatt caacaaagcc acgttgtgtc tcaaaatctc tgatgttaca 5101 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta 5161 atacaagggg tgtt //