SynBioHub

Created by: Trevor Y. H. Ho,Baojun Wang

pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry(1-192)-GAEY-e-NpuNcage-FKBP-T-cat-PBAD(SapI-)-RiboJ-BCD1-FRB-NpuCcage-CFNS-mCherry(193-236)-H6-Ter

Details

Types	circular DnaRegion
Roles	engineered_plasmid
Sequences	IBMc334_seq (Version 1)

Description

pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-GAEY-e-NpuNcage-FKBP-T-cat-PBAD(SapI-)-RiboJ-BCD1-FRB-NpuCcage-CFNS-mCherry*(193-236)-H6-Ter
Expression plasmid for mCherry split at 192/193 inserted with Npu DnaE, caged intein fused with FKBP/FRB

Notes

Creator: Trevor Y. H. Ho
Principal Investigator: Baojun Wang
BioSafety Level: Level 1
Backbone: pSB3T5
Origin of replication: p15A
Selection Markers: Tetracycline/ Chloramphenicol

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
ECK120033737 tetR promoter BCD1 NpuN(E52K,E54K,D85K,E89R,E91K) RBS for CDS2 Caspase3 cut site BBa_B0030 RBS for CDS 1 p15A origin of replication RiboJ FRB E coli optimized VR NpuC PBAD(SapI-) RFC10 Suffix VF2 L3S2P55 (NpuC1-30(K2E,R6E,K11E))x2 ECK120033736 cat promoter H6 mCherry(1-192) cat (BsmBI-) ECK120029600 mCherry(193-236) PBAD(SapI-) RFC10 Prefix (full) NpuN(51-102) FKBP E coli optimized tetR Caspase3 cut site araC	complement(4314,4370) 9,97 5605,5689 3323,3628 5675,5683 6158,6169 2714,2728 5624,5631 7097,7921 5530,5604 5693,5971 complement(6631,6650) 6185,6298 5241,5524 6516,6536 1371,1390 5165,5221 3680,3862 6463,6515 complement(5059,5156) 6434,6451 2735,3309 complement(4399,5058) 4216,4305 6302,6433 2422,2705 1494,1515 5987,6142 3884,4204 98,1291 3644,3655 complement(1516,2394)	terminator promoter biological_region CDS ribosome_entry_site CDS ribosome_entry_site ribosome_entry_site origin_of_replication insulator CDS primer_binding_site CDS promoter biological_region primer_binding_site terminator CDS terminator promoter CDS CDS CDS terminator CDS promoter biological_region CDS CDS CDS CDS CDS

Other Properties

genbank#date	18-NOV-2020
genbank#molecule	ds-DNA
synbiohub#ownedBy	user/trevorho
synbiohub#topLevel	IBMc334/1

Member of these Collections

Intein_assisted_Bisection_Mapping

IBMc334

IBMc334 Version 1

Component

Created by: Trevor Y. H. Ho,Baojun Wang

pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry(1-192)-GAEY-e-NpuNcage-FKBP-T-cat-PBAD(SapI-)-RiboJ-BCD1-FRB-NpuCcage-CFNS-mCherry(193-236)-H6-Ter

Details

Description

Notes

Sequence Annotations

Other Properties

Member of these Collections

Attachments

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