LOCUS IBMc334 8252 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-GAEY-e-NpuNcage-FKBP- T-cat-PBAD(SapI-)-RiboJ-BCD1-FRB-NpuCcage-CFNS-mCherry*(193-236)-H6 -Ter ACCESSION IBMc334 VERSION IBMc334.1 FEATURES Location/Qualifiers terminator complement(4314..4370) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" CDS 6185..6298 /ApEinfo_fwdcolor="#f8d3a9" /ApEinfo_revcolor="#f8d3a9" /label="NpuC" terminator 4216..4305 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" CDS 5987..6142 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="NpuN(51-102)" CDS 3884..4204 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="FKBP E coli optimized" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" CDS 3644..3655 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="Caspase3 cut site" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" misc_feature 5605..5689 /ApEinfo_fwdcolor="#008000" /ApEinfo_revcolor="#008000" /label="BCD1" CDS 3323..3628 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="NpuN(E52K,E54K,D85K,E89R,E91K)" RBS 5675..5683 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="RBS for CDS2" CDS 6158..6169 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="Caspase3 cut site" RBS 2714..2728 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" RBS 5624..5631 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="RBS for CDS 1" rep_origin 7097..7921 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" misc_feature 5530..5604 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="RiboJ" /featureType="insulator" CDS 5693..5971 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="FRB E coli optimized" primer_bind complement(6631..6650) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" promoter 5241..5524 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" misc_feature 6516..6536 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" terminator 5165..5221 /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="L3S2P55" CDS 3680..3862 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="(NpuC1-30(K2E,R6E,K11E))x2" terminator 6463..6515 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" promoter complement(5059..5156) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" CDS 6434..6451 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="H6" CDS 2735..3309 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(1-192)" CDS complement(4399..5058) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" CDS 6302..6433 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(193-236)" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcAct tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagattaaag aggagaaata ctagatggtg agcaagggcg aagaaGATaa 2761 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg 2821 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 2881 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca 2941 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 3001 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 3061 ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca aggtgaagct 3121 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagAAAacca tgggctggga 3181 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag 3241 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa 3301 gaagcccGTT GGAGCAGAAT ATTGCCTGAG CTATGAAACC GAAATTCTGA CCGTTGAATA 3361 TGGTCTGCTG CCGATTGGTA AAATTGTGGA AAAACGTATT GAGTGCACCG TGTATAGCGT 3421 GGATAATAAC GGTAACATTT ATACCCAGCC GGTTGCACAG TGGCATGATC GTGGTAAACA 3481 GAAAGTTTTT GAGTACTGTC TGGAAGATGG TAGCCTGATT CGTGCAACCA AAGATCACAA 3541 ATTTATGACC GTGGATGGTC AGATGCTGCC CATTAAAGAA ATTTTTCGTC GTAAACTGGA 3601 TCTGATGCGT GTTGATAATC TGCCGAATGA AAATCTGTAT GGTGATGAAG TGGATGGCTA 3661 TTTTCAGGGT ATTGAAATTG CCACCGAAAA ATATCTGGGT GAGCAGAACG TTTATGATAT 3721 TGGTGTTGAA CGCGATCATA ACTTCGCACT GAAAAATGGT GGTTATTTCC AGGGCATCGA 3781 AATCGCAACA GAGAAATACC TGGGCGAACA GAATGTGTAT GACATCGGCG TGGAACGTGA 3841 TCACAATTTT GCCCTGAAGA ATGGCGGTAG CGGTAGCGCA AGCGGAGTTC AGGTAGAGAC 3901 AATTAGTCCT GGGGATGGCC GCACATTTCC GAAACGCGGC CAAACCTGTG TGGTACACTA 3961 TACAGGAATG TTGGAAGATG GCAAAAAGTT TGATTCCAGT CGTGATCGCA ACAAGCCTTT 4021 TAAATTTATG TTGGGAAAAC AGGAGGTAAT TCGCGGTTGG GAGGAAGGTG TCGCACAAAT 4081 GTCCGTGGGT CAACGCGCCA AGTTGACGAT CTCACCTGAT TACGCTTACG GAGCCACCGG 4141 CCATCCTGGC ATTATTCCCC CACATGCCAC TTTGGTCTTT GATGTTGAAC TTTTAAAGTT 4201 AGAAtaaTAC TAGAGttgag aagagaaaag aaaaccgccg atcctgtcca ccgcattact 4261 gcaaggtagt ggacaagacc ggcggtctta agttttttgg ctgaaTACTA GAGcctttgg 4321 tcgAAAAAAA AAGCCCGCAC TGTCAGGTGC GGGCTTTTTT ctgtgtttcc TACTAGAGCA 4381 AGCGAGCTCG ATATCAAATT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC 4441 ATTAAGCATT CTGCCGACAT GGAAGCCATC ACAAACGGCA TGATGAACCT GAATCGCCAG 4501 CGGCATCAGC ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA 4561 GAAGTTGTCC ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC 4621 TGAAACGAAA AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA 4681 ACACGCCACA TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT 4741 CCAGAGCGAT GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT 4801 ATCCCATATC ACCAGCTCAC CGTCTTTCAT TGCCATACGA AATTCCGGAT GAGCATTCAT 4861 CAGGCGGGCA AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT 4921 CTTTAAAAAG GCCGTAATAT CCAGCTGAAC GGTCTGGTTA TAGGTACATT GAGCAACTGA 4981 CTGAAATGCC TCAAAATGTT CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC 5041 AGTGATTTTT TTCTCCATTT TAGCTTCCTT AGCTCCTGAA AATCTCGATA ACTCAAAAAA 5101 TACGCCCGGT AGTGATCTTA TTTCATTATG GTGAAAGTTG GAACCTCTTA CGTGCCTACT 5161 AGAGCTCGGT ACCAAAGACG AACAATAAGA CGCTGAAAAG CGTCTTTTTT CGTTTTGGTC 5221 Cgcttaacga tcgttggctg agaaaccaat tgtccatatt gcatcagaca ttgccgtcac 5281 tgcgtctttt actggcActt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt 5341 ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac 5401 ggcagaaaag tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt 5461 ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct 5521 ccatacccga gctgtcaccg gatgtgcttt ccggtctgat gagtccgtga ggacgaaaca 5581 gcctctacaa ataattttgt ttaaGGGCCC AAGTTCACTT AAAAAGGAGA TCAACAATGA 5641 AAGCAATTTT CGTACTGAAA CATCTTAATC ATGCACAGGA GACTTTCTAa tgGCCGTGTT 5701 ATGGCACGAA CAGTGGTATG AGGGTTTGGA TGACGCATCG CGCCAGTTCT TCGGAGAACA 5761 TAACACCGAA AAAATGTTTG CGGCACTGGA ACCTCTTTAT GAGATGCTTA AACGTGGGCC 5821 TGAAACGTTG CGTGAGATTT CTTTTCAGAA CTCATTTGGG CGTGATTTGA ACGATGCCTA 5881 CGAATGGCTG ATGAATTACA AAAAGTCGAA AGATGTATCT AACCTGAATC AAGCATGGGA 5941 TATTTACTAT AATGTCTTTC GTAAAATCGG CGGTAATGGT AATGGTGGTG AACAAGAAGT 6001 GTTTGAGTAT TGTCTGGAAG ATGGTAGCCT GATTCGTGCA ACCAAAGATC ACAAATTTAT 6061 GACCGTTGAT GGTCAGATGC TGCCGATTGA TGAAATTTTT GAACGTGAAC TGGATCTGAT 6121 GCGTGTTGAT AATCTGCCGA ATGAAAATCT GTATGGTGAT GAAGTGGATG GCTATTTTCA 6181 GGGTATCAAA ATTGCGACCC GTAAATATCT GGGTAAACAG AACGTTTATG ATATTGGTGT 6241 GGAACGCGAT CATAACTTTG CACTGAAAAA TGGTTTTATC GCCAGCAACT GCTTCAATTC 6301 Acagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga 6361 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga 6421 cgagctgtac aagcatcatc accatcacca ctaatactag agaacgcatg agAAAGCCCC 6481 CGGAAGATCA CCTTCCGGGG GCTTTtttat tgcgctacta gtagcggccg ctgcaggagt 6541 cactaagggt tagttagtta gattagcaga aagtcaaaag cctccgaccg gaggcttttg 6601 actaaaactt cccttggggt tatcattggg gctcactcaa aggcggtaat cagataaaaa 6661 aaatccttag ctttcgctaa ggatgatttc tgctagagat ggaatagact ggatggaggc 6721 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 6781 taaatctgga gccggtgagc gtggatctcg cggtatcatt gcagcactgg ggccagatgg 6841 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 6901 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 6961 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 7021 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 7081 ctgagcgtca gaccccttaa taagatgatc ttcttgagat cgttttggtc tgcgcgtaat 7141 ctcttgctct gaaaacgaaa aaaccgcctt gcagggcggt ttttcgaagg ttctctgagc 7201 taccaactct ttgaaccgag gtaactggct tggaggagcg cagtcaccaa aacttgtcct 7261 ttcagtttag ccttaaccgg cgcatgactt caagactaac tcctctaaat caattaccag 7321 tggctgctgc cagtggtgct tttgcatgtc tttccgggtt ggactcaaga cgatagttac 7381 cggataaggc gcagcggtcg gactgaacgg ggggttcgtg catacagtcc agcttggagc 7441 gaactgccta cccggaactg agtgtcaggc gtggaatgag acaaacgcgg ccataacagc 7501 ggaatgacac cggtaaaccg aaaggcagga acaggagagc gcacgaggga gccgccaggg 7561 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga 7621 tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa cggctttgcc gcggccctct 7681 cacttccctg ttaagtatct tcctggcatc ttccaggaaa tctccgcccc gttcgtaagc 7741 catttccgct cgccgcagtc gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga 7801 atatatcctg tatcacatat tctgctgacg caccggtgca gccttttttc tcctgccaca 7861 tgaagcactt cactgacacc ctcatcagtg ccaacatagt aagccagtat acactccgct 7921 agcgctgagg tctgcctcgt gaagaaggtg ttgctgactc ataccaggcc tgaatcgccc 7981 catcatccag ccagaaagtg agggagccac ggttgatgag agctttgttg taggtggacc 8041 agttggtgat tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg 8101 tgatctgatc cttcaactca gcaaaagttc gatttattca acaaagccac gttgtgtctc 8161 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt 8221 ctgcttacat aaacagtaat acaaggggtg tt //