LOCUS IBMc360 7997 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry(1-192)-G-M86(1-100)-10aa-acVH H-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(101-154)-S-mCherry(19 3-236)-H6-T ACCESSION IBMc360 VERSION IBMc360.1 FEATURES Location/Qualifiers CDS 3314..3613 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86N" primer_bind complement(6376..6395) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" misc_feature 5407..5491 /ApEinfo_fwdcolor="#008000" /ApEinfo_revcolor="#008000" /label="BCD1" misc_feature 5332..5406 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="RiboJ" /featureType="insulator" RBS 2714..2728 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" rep_origin 6842..7666 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" promoter 5043..5326 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" misc_feature 6261..6281 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" terminator 4967..5023 /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="L3S2P55" terminator 6208..6260 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" RBS 5426..5433 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="RBS for CDS 1" promoter complement(4861..4958) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" CDS 6179..6196 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="H6" CDS complement(4201..4860) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" CDS 6047..6178 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(193-236)" CDS 2735..3310 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(1-192)" terminator complement(4116..4172) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" CDS 5882..6043 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86C" terminator 4018..4107 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" CDS 5852..5881 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="2x(GGGGS) linker" CDS 3650..4006 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="acVHH" CDS 5495..5851 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="acVHH" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" CDS 3620..3649 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="2x(GGGGS) linker" RBS 5477..5485 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="RBS for CDS2" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcAct tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagattaaag aggagaaata ctagatggtg agcaagggcg aagaaGATaa 2761 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg 2821 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 2881 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca 2941 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 3001 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 3061 ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca aggtgaagct 3121 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagAAAacca tgggctggga 3181 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag 3241 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa 3301 gaagcccgtg GGAtgcatct cgggagatag tttgatcagc ttggcgagca cagggaaaag 3361 agttcctatt aaggatttgt taggcgaaaa agattttgaa atatgggcaa ttaatgaaca 3421 gacgatgaag ctggaatcag ctaaagttag tcgtgtattt tgtaccggca aaaagctagt 3481 ctatactcta aaaactcgac taggtagaac tatcaaggca acagcaaatc atagattttt 3541 aactattgat ggttggaaaa gattagatga gctatcttta aaagagcata ttgctctacc 3601 ccgtaaacta gaaagctcTG GAGGCGGTGG TAGCGGTGGT GGCGGTAGCG AAGTTCAGCT 3661 GCAAGCAAGC GGTGGTGGTC TGGTTCAGGC AGGCGGTAGC CTGCGTCTGA GCTGTACCGC 3721 AAGCGGTCGT ACCGGCACCA TTTATAGCAT GGCATGGTTT CGTCAGGCAC CGGGTAAAGA 3781 ACGTGAATTT CTGGCAACCG TTGGTTGGAG CAGCGGTATT ACCTATTATA TGGATAGCGT 3841 GAAAGGTCGC TTTACCATTA GCCGTGATAA TGCAAAAAAT AGCGCCTACC TGCAAATGAA 3901 TAGCCTGAAA CCGGAAGATA CCGCAGTGTA TTATTGTACA GCAACCCGTG CATATAGCGT 3961 GGGTTATGAT TATTGGGGTC AGGGCACCCA GGTTACCGTT AGCCATtaaT ACTAGAGttg 4021 agaagagaaa agaaaaccgc cgatcctgtc caccgcatta ctgcaaggta gtggacaaga 4081 ccggcggtct taagtttttt ggctgaaTAC TAGAGccttt ggtcgAAAAA AAAAGCCCGC 4141 ACTGTCAGGT GCGGGCTTTT TTctgtgttt ccTACTAGAG CAAGCGAGCT CGATATCAAA 4201 TTACGCCCCG CCCTGCCACT CATCGCAGTA CTGTTGTAAT TCATTAAGCA TTCTGCCGAC 4261 ATGGAAGCCA TCACAAACGG CATGATGAAC CTGAATCGCC AGCGGCATCA GCACCTTGTC 4321 GCCTTGCGTA TAATATTTGC CCATGGTGAA AACGGGGGCG AAGAAGTTGT CCATATTGGC 4381 CACGTTTAAA TCAAAACTGG TGAAACTCAC CCAGGGATTG GCTGAAACGA AAAACATATT 4441 CTCAATAAAC CCTTTAGGGA AATAGGCCAG GTTTTCACCG TAACACGCCA CATCTTGCGA 4501 ATATATGTGT AGAAACTGCC GGAAATCGTC GTGGTATTCA CTCCAGAGCG ATGAAAACGT 4561 TTCAGTTTGC TCATGGAAAA CGGTGTAACA AGGGTGAACA CTATCCCATA TCACCAGCTC 4621 ACCGTCTTTC ATTGCCATAC GAAATTCCGG ATGAGCATTC ATCAGGCGGG CAAGAATGTG 4681 AATAAAGGCC GGATAAAACT TGTGCTTATT TTTCTTTACG GTCTTTAAAA AGGCCGTAAT 4741 ATCCAGCTGA ACGGTCTGGT TATAGGTACA TTGAGCAACT GACTGAAATG CCTCAAAATG 4801 TTCTTTACGA TGCCATTGGG ATATATCAAC GGTGGTATAT CCAGTGATTT TTTTCTCCAT 4861 TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT 4921 TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCTA CTAGAGCTCG GTACCAAAGA 4981 CGAACAATAA GACGCTGAAA AGCGTCTTTT TTCGTTTTGG TCCgcttaac gatcgttggc 5041 tgagaaacca attgtccata ttgcatcaga cattgccgtc actgcgtctt ttactggcAc 5101 ttctcgctaa ccaaaccggt aaccccgctt attaaaagca ttctgtaaca aagcgggacc 5161 aaagccatga caaaaacgcg taacaaaagt gtctataatc acggcagaaa agtccacatt 5221 gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat aagattagcg 5281 gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccc gagctgtcac 5341 cggatgtgct ttccggtctg atgagtccgt gaggacgaaa cagcctctac aaataatttt 5401 gtttaaGGGC CCAAGTTCAC TTAAAAAGGA GATCAACAAT GAAAGCAATT TTCGTACTGA 5461 AACATCTTAA TCATGCACAG GAGACTTTCT AatgGAAGTT CAGCTGCAAG CAAGCGGTGG 5521 TGGTCTGGTT CAGGCAGGCG GTAGCCTGCG TCTGAGCTGT ACCGCAAGCG GTCGTACCGG 5581 CACCATTTAT AGCATGGCAT GGTTTCGTCA GGCACCGGGT AAAGAACGTG AATTTCTGGC 5641 AACCGTTGGT TGGAGCAGCG GTATTACCTA TTATATGGAT AGCGTGAAAG GTCGCTTTAC 5701 CATTAGCCGT GATAATGCAA AAAATAGCGC CTACCTGCAA ATGAATAGCC TGAAACCGGA 5761 AGATACCGCA GTGTATTATT GTACAGCAAC CCGTGCATAT AGCGTGGGTT ATGATTATTG 5821 GGGTCAGGGC ACCCAGGTTA CCGTTAGCCA TGGTGGAGGT GGTTCAGGTG GCGGAGGTTC 5881 Aagctcctct ttacaattgg caccagaaat agaaaagttg cctcagagtg atatttactg 5941 ggaccccatc gtttctatta cggagactgg agtcgaagag gtttttgatt tgactgtgcc 6001 aggactacgt aactttgtcg ccaatgacat cattgtacat aacTCAcagc tgcccggcgc 6061 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga 6121 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagca 6181 tcatcaccat caccactaat actagagaac gcatgagAAA GCCCCCGGAA GATCACCTTC 6241 CGGGGGCTTT tttattgcgc tactagtagc ggccgctgca ggagtcacta agggttagtt 6301 agttagatta gcagaaagtc aaaagcctcc gaccggaggc ttttgactaa aacttccctt 6361 ggggttatca ttggggctca ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc 6421 gctaaggatg atttctgcta gagatggaat agactggatg gaggcggata aagttgcagg 6481 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 6541 tgagcgtgga tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 6601 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 6661 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 6721 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 6781 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 6841 cttaataaga tgatcttctt gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa 6901 cgaaaaaacc gccttgcagg gcggtttttc gaaggttctc tgagctacca actctttgaa 6961 ccgaggtaac tggcttggag gagcgcagtc accaaaactt gtcctttcag tttagcctta 7021 accggcgcat gacttcaaga ctaactcctc taaatcaatt accagtggct gctgccagtg 7081 gtgcttttgc atgtctttcc gggttggact caagacgata gttaccggat aaggcgcagc 7141 ggtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg 7201 aactgagtgt caggcgtgga atgagacaaa cgcggccata acagcggaat gacaccggta 7261 aaccgaaagg caggaacagg agagcgcacg agggagccgc caggggaaac gcctggtatc 7321 tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt 7381 caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag 7441 tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg 7501 cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca 7561 catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag cacttcactg 7621 acaccctcat cagtgccaac atagtaagcc agtatacact ccgctagcgc tgaggtctgc 7681 ctcgtgaaga aggtgttgct gactcatacc aggcctgaat cgccccatca tccagccaga 7741 aagtgaggga gccacggttg atgagagctt tgttgtaggt ggaccagttg gtgattttga 7801 acttttgctt tgccacggaa cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca 7861 actcagcaaa agttcgattt attcaacaaa gccacgttgt gtctcaaaat ctctgatgtt 7921 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca 7981 gtaatacaag gggtgtt //