LOCUS IBMc367 2633 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB1C3(BsmBI-,BsaI+)-BsaI-ECF20(6-188)-BsaI_v3 ACCESSION IBMc367 VERSION IBMc367.1 FEATURES Location/Qualifiers primer_bind complement(1848..1867) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" CDS complement(221..880) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" terminator complement(106..200) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="lambda t0 terminator" primer_bind 992..1011 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" misc_feature 1110..1131 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" misc_feature 1694..1714 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" promoter complement(881..978) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" CDS 1139..1685 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="ECF20" rep_origin complement(1954..2568) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="rep (pMB1)" ORIGIN 1 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 61 taaagtatat atgagtaaac ttggtctcga cagctcgagg cttggattct caccaataaa 121 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac 181 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact 241 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg 301 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc 361 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg 421 tgaaactcac ccagggattg gctgaAACga aaaacatatt ctcaataaac cctttaggga 481 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc 541 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa 601 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac 661 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact 721 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt 781 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg 841 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg 901 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt 961 tggaacctct tacgtgcccg atcaactcga gtgccacctg acgtctaaga aaccattatt 1021 atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa 1081 tccttagctt tcgctaagga tgatttctgg aattcgcggc cgcttctaga gGGTCTCAat 1141 cctgatctgg aactgctgaa acgtattggt aataatgatg cacaggccgt taaagaaatg 1201 gttacccgta aactgcctcg tctgctggca ctggcaagtc gcctgctggg tgatgcagat 1261 gaagcacgtg atattgcaca agaaagtttt ctgcgcattt ggaaacaggc agcaagctgg 1321 cgtagcgaac aggcacgttt tgatacctgg ctgcatcgtg ttgcactgaa tctgtgttat 1381 gatcgtctgc gtcgtcgtaa agaacatgtg ccggttgata gcgaacatgc ctgtgaagca 1441 ctggataccc gtccggcacc ggatgaacag ctggaagcaa gcgcacagag ccgtcgtatg 1501 gcacaggcac tggatcagct gccggatcgt cagcgtgaag caattgttct gcagtattat 1561 caagaactga gcaataccga agcagcagca ctgatgcaaa ttagcgttga agccctggaa 1621 agcctgctga gccgtgcacg tcgtaatctg cgtagccatc tggccgaagc accgggtgca 1681 gatctgTGAG ACCtactagt agcggccgct gcagtccggc aaaaaagggc aaggtgtcac 1741 caccctgccc tttttcttta aaaccgaaaa gattacttcg cgttatgcag gcttcctcgc 1801 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 1861 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 1921 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc 1981 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 2041 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 2101 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 2161 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 2221 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 2281 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 2341 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 2401 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 2461 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 2521 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 2581 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg aga //