LOCUS IBMc378 7997 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-17)-PI-10aa-a cVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(18-154)-S-mCherry* (193-236)-H6-T ACCESSION IBMc378 VERSION IBMc378.1 FEATURES Location/Qualifiers misc_feature 5075..5149 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="RiboJ" /featureType="insulator" primer_bind complement(6368..6387) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" primer_bind 1363..1382 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" promoter 4786..5069 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" misc_feature 6253..6273 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" terminator 4710..4766 /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="L3S2P55" promoter 1..89 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" terminator 6200..6252 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" CDS 2727..3302 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(1-192)" CDS complement(3944..4603) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" CDS 6171..6188 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="H6" terminator complement(3859..3915) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" promoter 2414..2697 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" CDS 6039..6170 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(193-236)" terminator 3761..3850 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" CDS 5625..6035 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86(18-154)" misc_feature 1486..1507 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" CDS 3393..3749 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="acVHH" CDS 5595..5624 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="2x(GGGGS) linker" CDS 90..1283 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" CDS 3363..3392 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="2x(GGGGS) linker" CDS 5238..5594 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="acVHH" CDS 3306..3356 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86(1-17)" RBS 5220..5228 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="RBS for CDS2" rep_origin 6834..7658 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" RBS 2706..2720 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" misc_feature 5150..5234 /ApEinfo_fwdcolor="#008000" /ApEinfo_revcolor="#008000" /label="BCD1" RBS 5169..5176 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="RBS for CDS 1" promoter complement(4604..4701) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" CDS complement(1508..2386) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" ORIGIN 1 gagattctca tgtttgacag cttatcatcg ataagcttta atgcggtagt ttatcacagt 61 taaattgcta acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc atcgtcattc 121 tcggcaccgt caccctggac gctgtaggca taggcttggt tatgccggta ctgccgggcc 181 tcttgcggga tatcgtccat tccgacagta ttgccagtca ctatggcgtg ctgcttgcgc 241 tctatgcgtt gatgcaattt ctttgcgcac ccgttctcgg agccctgtcc gaccgctttg 301 gccgccgtcc agtcctgctc gcttcgctcc ttggagccac tatcgactac gcgatcatgg 361 cgaccacacc cgtcctgtgg attctctacg ccggacgcat cgtggcgggc atcacgggtg 421 ccacaggtgc ggttgctggt gcctatatcg ccgacatcac cgatggggaa gatcgggctc 481 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg 541 ggggactgtt gggtgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg 601 gcctcaacct cctcctgggc tgcttcctta tgcaggaatc gcataaggga gagcgccgtc 661 cgatgccctt gcgtgccttc aatccagtca gctccttccg gtgggcgcgg ggcatgacta 721 tcgtcgccgc acttatgact gttttcttta tcatgcaact cgtaggacag gttccggcag 781 cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt 841 cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcacg ggccccgcca 901 ccaaacgttt cggcgagaag caggccatta tcgcgggcat ggcggccgac gcgctgggct 961 acgtcttgct ggcgttcgcg acgcgcggct ggatggcctt ccccattatg attcttctcg 1021 cttccggcgg catcggtatg cccgcgttgc aggccatgct gtcccgccaa gtagatgacg 1081 accatcaggg acagcttcaa gggtcgctcg cggctcttac cagcctcact tcgatcattg 1141 gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat 1201 ggattgtagg tgccgccctt taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga 1261 gccgggccac ctcgacctaa taatactagc tccggcaaaa aaacgggcaa ggtgtcacca 1321 ccctgccctt tttctttaaa accgaaaaga ttacttcgcg tttgccacct gacgtctaag 1381 aaaaggaata ttcagcaatt tgcccgtgcc gaagaaaggc ccacccgtga aggtgagcca 1441 gtgagttgat tgctacgtaa ttagttagtt agcccttagt gactcgaatt cgcggccgct 1501 tctagagtta tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga 1561 actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt 1621 caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct 1681 tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc 1741 ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat 1801 caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat 1861 ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa 1921 gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt 1981 gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat 2041 tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa 2101 cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg 2161 gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga tttttcacca 2221 ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga 2281 taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat 2341 taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc 2401 cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt cactgcgtct 2461 tttactggcA cttctcgcta accaaaccgg taaccccgct tattaaaagc attctgtaac 2521 aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat cacggcagaa 2581 aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca 2641 taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccattac 2701 tagagattaa agaggagaaa tactagatgg tgagcaaggg cgaagaaGAT aacatggcca 2761 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt 2821 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga 2881 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt 2941 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct 3001 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg 3061 tgacccagga ctcctccttg caggacggcg agttcatcta caaggtgaag ctgcgcggca 3121 ccaacttccc ctccgacggc cccgtaatgc agaagAAAac catgggctgg gaggcctcct 3181 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc 3241 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg 3301 tgGGAtgcat ctcgggagat agtttgatca gcttggcgag cacagggaaa agagttccta 3361 tTGGAGGCGG TGGTAGCGGT GGTGGCGGTA GCGAAGTTCA GCTGCAAGCA AGCGGTGGTG 3421 GTCTGGTTCA GGCAGGCGGT AGCCTGCGTC TGAGCTGTAC CGCAAGCGGT CGTACCGGCA 3481 CCATTTATAG CATGGCATGG TTTCGTCAGG CACCGGGTAA AGAACGTGAA TTTCTGGCAA 3541 CCGTTGGTTG GAGCAGCGGT ATTACCTATT ATATGGATAG CGTGAAAGGT CGCTTTACCA 3601 TTAGCCGTGA TAATGCAAAA AATAGCGCCT ACCTGCAAAT GAATAGCCTG AAACCGGAAG 3661 ATACCGCAGT GTATTATTGT ACAGCAACCC GTGCATATAG CGTGGGTTAT GATTATTGGG 3721 GTCAGGGCAC CCAGGTTACC GTTAGCCATt aaTACTAGAG ttgagaagag aaaagaaaac 3781 cgccgatcct gtccaccgca ttactgcaag gtagtggaca agaccggcgg tcttaagttt 3841 tttggctgaa TACTAGAGcc tttggtcgAA AAAAAAAGCC CGCACTGTCA GGTGCGGGCT 3901 TTTTTctgtg tttccTACTA GAGCAAGCGA GCTCGATATC AAATTACGCC CCGCCCTGCC 3961 ACTCATCGCA GTACTGTTGT AATTCATTAA GCATTCTGCC GACATGGAAG CCATCACAAA 4021 CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT GTCGCCTTGC GTATAATATT 4081 TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT GGCCACGTTT AAATCAAAAC 4141 TGGTGAAACT CACCCAGGGA TTGGCTGAAA CGAAAAACAT ATTCTCAATA AACCCTTTAG 4201 GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG CGAATATATG TGTAGAAACT 4261 GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA CGTTTCAGTT TGCTCATGGA 4321 AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG CTCACCGTCT TTCATTGCCA 4381 TACGAAATTC CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA 4441 ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT 4501 GGTTATAGGT ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT 4561 GGGATATATC AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATTTTAGCT TCCTTAGCTC 4621 CTGAAAATCT CGATAACTCA AAAAATACGC CCGGTAGTGA TCTTATTTCA TTATGGTGAA 4681 AGTTGGAACC TCTTACGTGC CTACTAGAGC TCGGTACCAA AGACGAACAA TAAGACGCTG 4741 AAAAGCGTCT TTTTTCGTTT TGGTCCgctt aacgatcgtt ggctgagaaa ccaattgtcc 4801 atattgcatc agacattgcc gtcactgcgt cttttactgg cActtctcgc taaccaaacc 4861 ggtaaccccg cttattaaaa gcattctgta acaaagcggg accaaagcca tgacaaaaac 4921 gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt 4981 cacactttgc tatgccatag catttttatc cataagatta gcggatccta cctgacgctt 5041 tttatcgcaa ctctctactg tttctccata cccgagctgt caccggatgt gctttccggt 5101 ctgatgagtc cgtgaggacg aaacagcctc tacaaataat tttgtttaaG GGCCCAAGTT 5161 CACTTAAAAA GGAGATCAAC AATGAAAGCA ATTTTCGTAC TGAAACATCT TAATCATGCA 5221 CAGGAGACTT TCTAatgGAA GTTCAGCTGC AAGCAAGCGG TGGTGGTCTG GTTCAGGCAG 5281 GCGGTAGCCT GCGTCTGAGC TGTACCGCAA GCGGTCGTAC CGGCACCATT TATAGCATGG 5341 CATGGTTTCG TCAGGCACCG GGTAAAGAAC GTGAATTTCT GGCAACCGTT GGTTGGAGCA 5401 GCGGTATTAC CTATTATATG GATAGCGTGA AAGGTCGCTT TACCATTAGC CGTGATAATG 5461 CAAAAAATAG CGCCTACCTG CAAATGAATA GCCTGAAACC GGAAGATACC GCAGTGTATT 5521 ATTGTACAGC AACCCGTGCA TATAGCGTGG GTTATGATTA TTGGGGTCAG GGCACCCAGG 5581 TTACCGTTAG CCATGGTGGA GGTGGTTCAG GTGGCGGAGG TTCAcctatt aaggatttgt 5641 taggcgaaaa agattttgaa atatgggcaa ttaatgaaca gacgatgaag ctggaatcag 5701 ctaaagttag tcgtgtattt tgtaccggca aaaagctagt ctatactcta aaaactcgac 5761 taggtagaac tatcaaggca acagcaaatc atagattttt aactattgat ggttggaaaa 5821 gattagatga gctatcttta aaagagcata ttgctctacc ccgtaaacta gaaagctcct 5881 ctttacaatt ggcaccagaa atagaaaagt tgcctcagag tgatatttac tgggacccca 5941 tcgtttctat tacggagact ggagtcgaag aggtttttga tttgactgtg ccaggactac 6001 gtaactttgt cgccaatgac atcattgtac ataacTCAca gctgcccggc gcctacaacg 6061 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg 6121 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag catcatcacc 6181 atcaccacta atactagaga acgcatgagA AAGCCCCCGG AAGATCACCT TCCGGGGGCT 6241 TTtttattgc gctactagta gcggccgctg caggagtcac taagggttag ttagttagat 6301 tagcagaaag tcaaaagcct ccgaccggag gcttttgact aaaacttccc ttggggttat 6361 cattggggct cactcaaagg cggtaatcag ataaaaaaaa tccttagctt tcgctaagga 6421 tgatttctgc tagagatgga atagactgga tggaggcgga taaagttgca ggaccacttc 6481 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 6541 gatctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 6601 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 6661 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 6721 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 6781 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccttaataa 6841 gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa 6901 ccgccttgca gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta 6961 actggcttgg aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc 7021 atgacttcaa gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt 7081 gcatgtcttt ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac 7141 tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt 7201 gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa 7261 ggcaggaaca ggagagcgca cgagggagcc gccaggggaa acgcctggta tctttatagt 7321 cctgtcgggt ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg 7381 cggagcctat ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta agtatcttcc 7441 tggcatcttc caggaaatct ccgccccgtt cgtaagccat ttccgctcgc cgcagtcgaa 7501 cgaccgagcg tagcgagtca gtgagcgagg aagcggaata tatcctgtat cacatattct 7561 gctgacgcac cggtgcagcc ttttttctcc tgccacatga agcacttcac tgacaccctc 7621 atcagtgcca acatagtaag ccagtataca ctccgctagc gctgaggtct gcctcgtgaa 7681 gaaggtgttg ctgactcata ccaggcctga atcgccccat catccagcca gaaagtgagg 7741 gagccacggt tgatgagagc tttgttgtag gtggaccagt tggtgatttt gaacttttgc 7801 tttgccacgg aacggtctgc gttgtcggga agatgcgtga tctgatcctt caactcagca 7861 aaagttcgat ttattcaaca aagccacgtt gtgtctcaaa atctctgatg ttacattgca 7921 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca 7981 aggggtgttt actagag //