LOCUS IBMc380 7997 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-39)-LD-10aa-a cVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(40-154)-S-mCherry* (193-236)-H6-T ACCESSION IBMc380 VERSION IBMc380.1 FEATURES Location/Qualifiers CDS 6171..6188 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="H6" CDS complement(4010..4669) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" promoter 2414..2697 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" CDS 6039..6170 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(193-236)" terminator complement(3925..3981) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" CDS 5691..6035 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86(40-154)" misc_feature 1486..1507 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" terminator 3827..3916 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" CDS 5661..5690 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="2x(GGGGS) linker" CDS 90..1283 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" CDS 3459..3815 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="acVHH" CDS 5304..5660 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="acVHH" CDS 3429..3458 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="2x(GGGGS) linker" RBS 5286..5294 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="RBS for CDS2" RBS 2706..2720 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" CDS 3306..3422 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86(1-39)" RBS 5235..5242 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="RBS for CDS 1" primer_bind complement(6368..6387) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" misc_feature 5216..5300 /ApEinfo_fwdcolor="#008000" /ApEinfo_revcolor="#008000" /label="BCD1" CDS complement(1508..2386) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" misc_feature 5141..5215 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="RiboJ" /featureType="insulator" rep_origin 6834..7658 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" promoter 4852..5135 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" primer_bind 1363..1382 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" misc_feature 6253..6273 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" terminator 4776..4832 /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="L3S2P55" promoter 1..89 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" terminator 6200..6252 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" promoter complement(4670..4767) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" CDS 2727..3302 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(1-192)" ORIGIN 1 gagattctca tgtttgacag cttatcatcg ataagcttta atgcggtagt ttatcacagt 61 taaattgcta acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc atcgtcattc 121 tcggcaccgt caccctggac gctgtaggca taggcttggt tatgccggta ctgccgggcc 181 tcttgcggga tatcgtccat tccgacagta ttgccagtca ctatggcgtg ctgcttgcgc 241 tctatgcgtt gatgcaattt ctttgcgcac ccgttctcgg agccctgtcc gaccgctttg 301 gccgccgtcc agtcctgctc gcttcgctcc ttggagccac tatcgactac gcgatcatgg 361 cgaccacacc cgtcctgtgg attctctacg ccggacgcat cgtggcgggc atcacgggtg 421 ccacaggtgc ggttgctggt gcctatatcg ccgacatcac cgatggggaa gatcgggctc 481 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg 541 ggggactgtt gggtgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg 601 gcctcaacct cctcctgggc tgcttcctta tgcaggaatc gcataaggga gagcgccgtc 661 cgatgccctt gcgtgccttc aatccagtca gctccttccg gtgggcgcgg ggcatgacta 721 tcgtcgccgc acttatgact gttttcttta tcatgcaact cgtaggacag gttccggcag 781 cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt 841 cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcacg ggccccgcca 901 ccaaacgttt cggcgagaag caggccatta tcgcgggcat ggcggccgac gcgctgggct 961 acgtcttgct ggcgttcgcg acgcgcggct ggatggcctt ccccattatg attcttctcg 1021 cttccggcgg catcggtatg cccgcgttgc aggccatgct gtcccgccaa gtagatgacg 1081 accatcaggg acagcttcaa gggtcgctcg cggctcttac cagcctcact tcgatcattg 1141 gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat 1201 ggattgtagg tgccgccctt taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga 1261 gccgggccac ctcgacctaa taatactagc tccggcaaaa aaacgggcaa ggtgtcacca 1321 ccctgccctt tttctttaaa accgaaaaga ttacttcgcg tttgccacct gacgtctaag 1381 aaaaggaata ttcagcaatt tgcccgtgcc gaagaaaggc ccacccgtga aggtgagcca 1441 gtgagttgat tgctacgtaa ttagttagtt agcccttagt gactcgaatt cgcggccgct 1501 tctagagtta tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga 1561 actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt 1621 caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct 1681 tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc 1741 ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat 1801 caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat 1861 ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa 1921 gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt 1981 gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat 2041 tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa 2101 cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg 2161 gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga tttttcacca 2221 ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga 2281 taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat 2341 taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc 2401 cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt cactgcgtct 2461 tttactggcA cttctcgcta accaaaccgg taaccccgct tattaaaagc attctgtaac 2521 aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat cacggcagaa 2581 aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca 2641 taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccattac 2701 tagagattaa agaggagaaa tactagatgg tgagcaaggg cgaagaaGAT aacatggcca 2761 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt 2821 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga 2881 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt 2941 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct 3001 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg 3061 tgacccagga ctcctccttg caggacggcg agttcatcta caaggtgaag ctgcgcggca 3121 ccaacttccc ctccgacggc cccgtaatgc agaagAAAac catgggctgg gaggcctcct 3181 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc 3241 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg 3301 tgGGAtgcat ctcgggagat agtttgatca gcttggcgag cacagggaaa agagttccta 3361 ttaaggattt gttaggcgaa aaagattttg aaatatgggc aattaatgaa cagacgatga 3421 agctggaTGG AGGCGGTGGT AGCGGTGGTG GCGGTAGCGA AGTTCAGCTG CAAGCAAGCG 3481 GTGGTGGTCT GGTTCAGGCA GGCGGTAGCC TGCGTCTGAG CTGTACCGCA AGCGGTCGTA 3541 CCGGCACCAT TTATAGCATG GCATGGTTTC GTCAGGCACC GGGTAAAGAA CGTGAATTTC 3601 TGGCAACCGT TGGTTGGAGC AGCGGTATTA CCTATTATAT GGATAGCGTG AAAGGTCGCT 3661 TTACCATTAG CCGTGATAAT GCAAAAAATA GCGCCTACCT GCAAATGAAT AGCCTGAAAC 3721 CGGAAGATAC CGCAGTGTAT TATTGTACAG CAACCCGTGC ATATAGCGTG GGTTATGATT 3781 ATTGGGGTCA GGGCACCCAG GTTACCGTTA GCCATtaaTA CTAGAGttga gaagagaaaa 3841 gaaaaccgcc gatcctgtcc accgcattac tgcaaggtag tggacaagac cggcggtctt 3901 aagttttttg gctgaaTACT AGAGcctttg gtcgAAAAAA AAAGCCCGCA CTGTCAGGTG 3961 CGGGCTTTTT Tctgtgtttc cTACTAGAGC AAGCGAGCTC GATATCAAAT TACGCCCCGC 4021 CCTGCCACTC ATCGCAGTAC TGTTGTAATT CATTAAGCAT TCTGCCGACA TGGAAGCCAT 4081 CACAAACGGC ATGATGAACC TGAATCGCCA GCGGCATCAG CACCTTGTCG CCTTGCGTAT 4141 AATATTTGCC CATGGTGAAA ACGGGGGCGA AGAAGTTGTC CATATTGGCC ACGTTTAAAT 4201 CAAAACTGGT GAAACTCACC CAGGGATTGG CTGAAACGAA AAACATATTC TCAATAAACC 4261 CTTTAGGGAA ATAGGCCAGG TTTTCACCGT AACACGCCAC ATCTTGCGAA TATATGTGTA 4321 GAAACTGCCG GAAATCGTCG TGGTATTCAC TCCAGAGCGA TGAAAACGTT TCAGTTTGCT 4381 CATGGAAAAC GGTGTAACAA GGGTGAACAC TATCCCATAT CACCAGCTCA CCGTCTTTCA 4441 TTGCCATACG AAATTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG 4501 GATAAAACTT GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTGAA 4561 CGGTCTGGTT ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT 4621 GCCATTGGGA TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCATT TTAGCTTCCT 4681 TAGCTCCTGA AAATCTCGAT AACTCAAAAA ATACGCCCGG TAGTGATCTT ATTTCATTAT 4741 GGTGAAAGTT GGAACCTCTT ACGTGCCTAC TAGAGCTCGG TACCAAAGAC GAACAATAAG 4801 ACGCTGAAAA GCGTCTTTTT TCGTTTTGGT CCgcttaacg atcgttggct gagaaaccaa 4861 ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggcAct tctcgctaac 4921 caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac 4981 aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca 5041 cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg 5101 acgcttttta tcgcaactct ctactgtttc tccatacccg agctgtcacc ggatgtgctt 5161 tccggtctga tgagtccgtg aggacgaaac agcctctaca aataattttg tttaaGGGCC 5221 CAAGTTCACT TAAAAAGGAG ATCAACAATG AAAGCAATTT TCGTACTGAA ACATCTTAAT 5281 CATGCACAGG AGACTTTCTA atgGAAGTTC AGCTGCAAGC AAGCGGTGGT GGTCTGGTTC 5341 AGGCAGGCGG TAGCCTGCGT CTGAGCTGTA CCGCAAGCGG TCGTACCGGC ACCATTTATA 5401 GCATGGCATG GTTTCGTCAG GCACCGGGTA AAGAACGTGA ATTTCTGGCA ACCGTTGGTT 5461 GGAGCAGCGG TATTACCTAT TATATGGATA GCGTGAAAGG TCGCTTTACC ATTAGCCGTG 5521 ATAATGCAAA AAATAGCGCC TACCTGCAAA TGAATAGCCT GAAACCGGAA GATACCGCAG 5581 TGTATTATTG TACAGCAACC CGTGCATATA GCGTGGGTTA TGATTATTGG GGTCAGGGCA 5641 CCCAGGTTAC CGTTAGCCAT GGTGGAGGTG GTTCAGGTGG CGGAGGTTCA ctggaatcag 5701 ctaaagttag tcgtgtattt tgtaccggca aaaagctagt ctatactcta aaaactcgac 5761 taggtagaac tatcaaggca acagcaaatc atagattttt aactattgat ggttggaaaa 5821 gattagatga gctatcttta aaagagcata ttgctctacc ccgtaaacta gaaagctcct 5881 ctttacaatt ggcaccagaa atagaaaagt tgcctcagag tgatatttac tgggacccca 5941 tcgtttctat tacggagact ggagtcgaag aggtttttga tttgactgtg ccaggactac 6001 gtaactttgt cgccaatgac atcattgtac ataacTCAca gctgcccggc gcctacaacg 6061 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg 6121 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag catcatcacc 6181 atcaccacta atactagaga acgcatgagA AAGCCCCCGG AAGATCACCT TCCGGGGGCT 6241 TTtttattgc gctactagta gcggccgctg caggagtcac taagggttag ttagttagat 6301 tagcagaaag tcaaaagcct ccgaccggag gcttttgact aaaacttccc ttggggttat 6361 cattggggct cactcaaagg cggtaatcag ataaaaaaaa tccttagctt tcgctaagga 6421 tgatttctgc tagagatgga atagactgga tggaggcgga taaagttgca ggaccacttc 6481 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 6541 gatctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 6601 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 6661 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 6721 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 6781 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccttaataa 6841 gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa 6901 ccgccttgca gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta 6961 actggcttgg aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc 7021 atgacttcaa gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt 7081 gcatgtcttt ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac 7141 tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt 7201 gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa 7261 ggcaggaaca ggagagcgca cgagggagcc gccaggggaa acgcctggta tctttatagt 7321 cctgtcgggt ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg 7381 cggagcctat ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta agtatcttcc 7441 tggcatcttc caggaaatct ccgccccgtt cgtaagccat ttccgctcgc cgcagtcgaa 7501 cgaccgagcg tagcgagtca gtgagcgagg aagcggaata tatcctgtat cacatattct 7561 gctgacgcac cggtgcagcc ttttttctcc tgccacatga agcacttcac tgacaccctc 7621 atcagtgcca acatagtaag ccagtataca ctccgctagc gctgaggtct gcctcgtgaa 7681 gaaggtgttg ctgactcata ccaggcctga atcgccccat catccagcca gaaagtgagg 7741 gagccacggt tgatgagagc tttgttgtag gtggaccagt tggtgatttt gaacttttgc 7801 tttgccacgg aacggtctgc gttgtcggga agatgcgtga tctgatcctt caactcagca 7861 aaagttcgat ttattcaaca aagccacgtt gtgtctcaaa atctctgatg ttacattgca 7921 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca 7981 aggggtgttt actagag //