LOCUS IBMc381 7738 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-159)-GAG-M86N-cat-Plux2-B3 2-M86C-S-mCherry*(160-236)-H6-T ACCESSION IBMc381 VERSION IBMc381.1 FEATURES Location/Qualifiers CDS 2735..3211 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" terminator 5391..5431 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0012" RBS 2714..2728 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" promoter complement(5356..5390) /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="BBa_J23117" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" misc_feature 6002..6022 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" rep_origin 6583..7407 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" CDS complement(4571..5326) /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="luxR" primer_bind complement(6117..6136) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" terminator complement(4481..4537) /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="L3S2P55" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" terminator 5949..6001 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" promoter complement(4375..4472) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" CDS 5920..5937 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="H6" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" CDS complement(3715..4374) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" CDS 5689..5919 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*" RBS complement(5333..5347) /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" terminator complement(3630..3686) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" CDS 5524..5685 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86C" terminator 3532..3621 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" RBS 5502..5514 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="BBa_B0032" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" CDS 3221..3520 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86N" promoter 5440..5493 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="Plux2" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcact tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagattaaag aggagaaata ctagatggtg agcaagggcg aagaaGATaa 2761 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg 2821 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 2881 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca 2941 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 3001 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 3061 ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca aggtgaagct 3121 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagAAAacca tgggctggga 3181 ggcctcctcc gagcggatgt accccgagga cggcgcTGGA tgcatctcgg gagatagttt 3241 gatcagcttg gcgagcacag ggaaaagagt tcctattaag gatttgttag gcgaaaaaga 3301 ttttgaaata tgggcaatta atgaacagac gatgaagctg gaatcagcta aagttagtcg 3361 tgtattttgt accggcaaaa agctagtcta tactctaaaa actcgactag gtagaactat 3421 caaggcaaca gcaaatcata gatttttaac tattgatggt tggaaaagat tagatgagct 3481 atctttaaaa gagcatattg ctctaccccg taaactagaa TAATACTAGA Gttgagaaga 3541 gaaaagaaaa ccgccgatcc tgtccaccgc attactgcaa ggtagtggac aagaccggcg 3601 gtcttaagtt ttttggctga aTACTAGAGc ctttggtcgA AAAAAAAAGC CCGCACTGTC 3661 AGGTGCGGGC TTTTTTctgt gtttccTACT AGAGCAAGCG AGCTCGATAT CAAATTACGC 3721 CCCGCCCTGC CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA 3781 GCCATCACAA ACGGCATGAT GAACCTGAAT CGCCAGCGGC ATCAGCACCT TGTCGCCTTG 3841 CGTATAATAT TTGCCCATGG TGAAAACGGG GGCGAAGAAG TTGTCCATAT TGGCCACGTT 3901 TAAATCAAAA CTGGTGAAAC TCACCCAGGG ATTGGCTGAA ACGAAAAACA TATTCTCAAT 3961 AAACCCTTTA GGGAAATAGG CCAGGTTTTC ACCGTAACAC GCCACATCTT GCGAATATAT 4021 GTGTAGAAAC TGCCGGAAAT CGTCGTGGTA TTCACTCCAG AGCGATGAAA ACGTTTCAGT 4081 TTGCTCATGG AAAACGGTGT AACAAGGGTG AACACTATCC CATATCACCA GCTCACCGTC 4141 TTTCATTGCC ATACGAAATT CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA 4201 GGCCGGATAA AACTTGTGCT TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG 4261 CTGAACGGTC TGGTTATAGG TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT 4321 ACGATGCCAT TGGGATATAT CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCATTTTAGC 4381 TTCCTTAGCT CCTGAAAATC TCGATAACTC AAAAAATACG CCCGGTAGTG ATCTTATTTC 4441 ATTATGGTGA AAGTTGGAAC CTCTTACGTG CCTACTAGAG GGACCAAAAC GAAAAAAGAC 4501 GCTTTTCAGC GTCTTATTGT TCGTCTTTGG TACCGAGCTC TAGTAGTGAT CTACACTAGC 4561 ACTATCAGTG TTATTAATTT TTAAAGTATG GGCAATCAAT TGCTCCTGTT AAAATTGCTT 4621 TAGAAATACT TTGGCAGCGG TTTGTTGTAT TGAGTTTCAT TTGCGCATTG GTTAAATGGA 4681 AAGTGACAGT ACGCTCACTG CAACCTAATA TTTTTGAAAT ATCCCAAGAG CTTTTTCCTT 4741 CGCATGCCCA CGCTAAACAT TCTTTTTCTC TTTTGGTTAA ATCGTTGTTT GATTTATTAT 4801 TTGCTATATT TATTTTTCGA TAATTATCAA CTAGAGAAGG AACAATTAAT GGTATGTTCA 4861 TACACGCATG TAAAAATAAA CTATCTATAT AGTTGTCTTT TTCTGAATGT GCAAAACTAA 4921 GCATTCCGAA GCCATTGTTA GCCGTATGAA TAGGGAAACT AAACCCAGTG ATAAGACCTG 4981 ATGTTTTCGC TTCTTTAATT ACATTTGGAG ATTTTTTATT TACAGCATTG TTTTCAAATA 5041 TATTCCAATT AATTGGTGAA TGATTGGAGT TAGAATAATC TACTATAGGA TCATATTTTA 5101 TTAAATTAGC GTCATCATAA TATTGCCTCC ATTTTTTAGG GTAATTATCT AGGATTGAAA 5161 TATCAGATTT AACCATAGAA TGAGGATAAA TGATCGCGAG TAAATAATAT TCACAATGTA 5221 CCATTTTAGT CATATCAGAT AAGCATTGAT TAATATCATT ATTGCTTCTA CAAGCTTTAA 5281 TTTTATTAAT TATTCTGTAT GTGTCGTCGG CATTTATGTT TTTCATCTAG TATTTCTCCT 5341 CTTTAATCTC TAGTAGCTAG CACAATCCCT AGGACTGAGC TAGCTGTCAA TCACACTGGC 5401 TCACCTTCGG GTGGGCCTTT CTGCGTTTAT ATACTAGACA CCTGTAGGAT CGTACAGGTT 5461 TACGCAAGAA AATGGTTTGT TACTTTCGAA TAAtactaga gtcacacagg aaagtactag 5521 ATGagctcct ctttacaatt ggcaccagaa atagaaaagt tgcctcagag tgatatttac 5581 tgggacccca tcgtttctat tacggagact ggagtcgaag aggtttttga tttgactgtg 5641 ccaggactac gtaactttgt cgccaatgac atcattgtac ataacTCAgg cgccctgaag 5701 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag 5761 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag 5821 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag 5881 ggccgccact ccaccggcgg catggacgag ctgtacaagc atcatcacca tcaccactaa 5941 tactagagaa cgcatgagAA AGCCCCCGGA AGATCACCTT CCGGGGGCTT Ttttattgcg 6001 ctactagtag cggccgctgc aggagtcact aagggttagt tagttagatt agcagaaagt 6061 caaaagcctc cgaccggagg cttttgacta aaacttccct tggggttatc attggggctc 6121 actcaaaggc ggtaatcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgct 6181 agagatggaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 6241 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg atctcgcggt 6301 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 6361 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 6421 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 6481 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 6541 atcccttaac gtgagttttc gttccactga gcgtcagacc ccttaataag atgatcttct 6601 tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag 6661 ggcggttttt cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgga 6721 ggagcgcagt caccaaaact tgtcctttca gtttagcctt aaccggcgca tgacttcaag 6781 actaactcct ctaaatcaat taccagtggc tgctgccagt ggtgcttttg catgtctttc 6841 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg 6901 ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg 6961 aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag 7021 gagagcgcac gagggagccg ccaggggaaa cgcctggtat ctttatagtc ctgtcgggtt 7081 tcgccaccac tgatttgagc gtcagatttc gtgatgcttg tcaggggggc ggagcctatg 7141 gaaaaacggc tttgccgcgg ccctctcact tccctgttaa gtatcttcct ggcatcttcc 7201 aggaaatctc cgccccgttc gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt 7261 agcgagtcag tgagcgagga agcggaatat atcctgtatc acatattctg ctgacgcacc 7321 ggtgcagcct tttttctcct gccacatgaa gcacttcact gacaccctca tcagtgccaa 7381 catagtaagc cagtatacac tccgctagcg ctgaggtctg cctcgtgaag aaggtgttgc 7441 tgactcatac caggcctgaa tcgccccatc atccagccag aaagtgaggg agccacggtt 7501 gatgagagct ttgttgtagg tggaccagtt ggtgattttg aacttttgct ttgccacgga 7561 acggtctgcg ttgtcgggaa gatgcgtgat ctgatccttc aactcagcaa aagttcgatt 7621 tattcaacaa agccacgttg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa 7681 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgtt //