LOCUS IBMc385 7741 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-160)-ALG-M86N-cat-Plux2-B3 2-M86C-S-mCherry*(161-236)-H6-T ACCESSION IBMc385 VERSION IBMc385.1 FEATURES Location/Qualifiers terminator 5952..6004 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" promoter complement(4378..4475) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" CDS 5923..5940 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="H6" CDS complement(3718..4377) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" primer_bind complement(6120..6139) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" CDS 5692..5922 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" terminator complement(3633..3689) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" CDS 5527..5688 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86C" terminator 3535..3624 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" RBS 5505..5517 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="BBa_B0032" CDS 3224..3523 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86N" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" promoter 5443..5496 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="Plux2" CDS 2735..3219 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*" terminator 5394..5434 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0012" RBS 2714..2728 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" promoter complement(5359..5393) /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="BBa_J23117" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" RBS complement(5336..5350) /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" rep_origin 6586..7410 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" CDS complement(4574..5329) /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="luxR" misc_feature 6005..6025 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" terminator complement(4484..4540) /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="L3S2P55" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcact tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagattaaag aggagaaata ctagatggtg agcaagggcg aagaaGATaa 2761 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg 2821 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 2881 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca 2941 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 3001 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 3061 ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca aggtgaagct 3121 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagAAAacca tgggctggga 3181 ggcctcctcc gagcggatgt accccgagga cggcgccctT GGAtgcatct cgggagatag 3241 tttgatcagc ttggcgagca cagggaaaag agttcctatt aaggatttgt taggcgaaaa 3301 agattttgaa atatgggcaa ttaatgaaca gacgatgaag ctggaatcag ctaaagttag 3361 tcgtgtattt tgtaccggca aaaagctagt ctatactcta aaaactcgac taggtagaac 3421 tatcaaggca acagcaaatc atagattttt aactattgat ggttggaaaa gattagatga 3481 gctatcttta aaagagcata ttgctctacc ccgtaaacta gaaTAATACT AGAGttgaga 3541 agagaaaaga aaaccgccga tcctgtccac cgcattactg caaggtagtg gacaagaccg 3601 gcggtcttaa gttttttggc tgaaTACTAG AGcctttggt cgAAAAAAAA AGCCCGCACT 3661 GTCAGGTGCG GGCTTTTTTc tgtgtttccT ACTAGAGCAA GCGAGCTCGA TATCAAATTA 3721 CGCCCCGCCC TGCCACTCAT CGCAGTACTG TTGTAATTCA TTAAGCATTC TGCCGACATG 3781 GAAGCCATCA CAAACGGCAT GATGAACCTG AATCGCCAGC GGCATCAGCA CCTTGTCGCC 3841 TTGCGTATAA TATTTGCCCA TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA TATTGGCCAC 3901 GTTTAAATCA AAACTGGTGA AACTCACCCA GGGATTGGCT GAAACGAAAA ACATATTCTC 3961 AATAAACCCT TTAGGGAAAT AGGCCAGGTT TTCACCGTAA CACGCCACAT CTTGCGAATA 4021 TATGTGTAGA AACTGCCGGA AATCGTCGTG GTATTCACTC CAGAGCGATG AAAACGTTTC 4081 AGTTTGCTCA TGGAAAACGG TGTAACAAGG GTGAACACTA TCCCATATCA CCAGCTCACC 4141 GTCTTTCATT GCCATACGAA ATTCCGGATG AGCATTCATC AGGCGGGCAA GAATGTGAAT 4201 AAAGGCCGGA TAAAACTTGT GCTTATTTTT CTTTACGGTC TTTAAAAAGG CCGTAATATC 4261 CAGCTGAACG GTCTGGTTAT AGGTACATTG AGCAACTGAC TGAAATGCCT CAAAATGTTC 4321 TTTACGATGC CATTGGGATA TATCAACGGT GGTATATCCA GTGATTTTTT TCTCCATTTT 4381 AGCTTCCTTA GCTCCTGAAA ATCTCGATAA CTCAAAAAAT ACGCCCGGTA GTGATCTTAT 4441 TTCATTATGG TGAAAGTTGG AACCTCTTAC GTGCCTACTA GAGGGACCAA AACGAAAAAA 4501 GACGCTTTTC AGCGTCTTAT TGTTCGTCTT TGGTACCGAG CTCTAGTAGT GATCTACACT 4561 AGCACTATCA GTGTTATTAA TTTTTAAAGT ATGGGCAATC AATTGCTCCT GTTAAAATTG 4621 CTTTAGAAAT ACTTTGGCAG CGGTTTGTTG TATTGAGTTT CATTTGCGCA TTGGTTAAAT 4681 GGAAAGTGAC AGTACGCTCA CTGCAACCTA ATATTTTTGA AATATCCCAA GAGCTTTTTC 4741 CTTCGCATGC CCACGCTAAA CATTCTTTTT CTCTTTTGGT TAAATCGTTG TTTGATTTAT 4801 TATTTGCTAT ATTTATTTTT CGATAATTAT CAACTAGAGA AGGAACAATT AATGGTATGT 4861 TCATACACGC ATGTAAAAAT AAACTATCTA TATAGTTGTC TTTTTCTGAA TGTGCAAAAC 4921 TAAGCATTCC GAAGCCATTG TTAGCCGTAT GAATAGGGAA ACTAAACCCA GTGATAAGAC 4981 CTGATGTTTT CGCTTCTTTA ATTACATTTG GAGATTTTTT ATTTACAGCA TTGTTTTCAA 5041 ATATATTCCA ATTAATTGGT GAATGATTGG AGTTAGAATA ATCTACTATA GGATCATATT 5101 TTATTAAATT AGCGTCATCA TAATATTGCC TCCATTTTTT AGGGTAATTA TCTAGGATTG 5161 AAATATCAGA TTTAACCATA GAATGAGGAT AAATGATCGC GAGTAAATAA TATTCACAAT 5221 GTACCATTTT AGTCATATCA GATAAGCATT GATTAATATC ATTATTGCTT CTACAAGCTT 5281 TAATTTTATT AATTATTCTG TATGTGTCGT CGGCATTTAT GTTTTTCATC TAGTATTTCT 5341 CCTCTTTAAT CTCTAGTAGC TAGCACAATC CCTAGGACTG AGCTAGCTGT CAATCACACT 5401 GGCTCACCTT CGGGTGGGCC TTTCTGCGTT TATATACTAG ACACCTGTAG GATCGTACAG 5461 GTTTACGCAA GAAAATGGTT TGTTACTTTC GAATAAtact agagtcacac aggaaagtac 5521 tagATGagct cctctttaca attggcacca gaaatagaaa agttgcctca gagtgatatt 5581 tactgggacc ccatcgtttc tattacggag actggagtcg aagaggtttt tgatttgact 5641 gtgccaggac tacgtaactt tgtcgccaat gacatcattg tacataacTC Aggcgccctg 5701 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc 5761 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc 5821 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc 5881 gagggccgcc actccaccgg cggcatggac gagctgtaca agcatcatca ccatcaccac 5941 taatactaga gaacgcatga gAAAGCCCCC GGAAGATCAC CTTCCGGGGG CTTTtttatt 6001 gcgctactag tagcggccgc tgcaggagtc actaagggtt agttagttag attagcagaa 6061 agtcaaaagc ctccgaccgg aggcttttga ctaaaacttc ccttggggtt atcattgggg 6121 ctcactcaaa ggcggtaatc agataaaaaa aatccttagc tttcgctaag gatgatttct 6181 gctagagatg gaatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 6241 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tggatctcgc 6301 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 6361 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 6421 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 6481 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 6541 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccttaat aagatgatct 6601 tcttgagatc gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg 6661 cagggcggtt tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt 6721 ggaggagcgc agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc 6781 aagactaact cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct 6841 ttccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg 6901 gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg 6961 tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa 7021 caggagagcg cacgagggag ccgccagggg aaacgcctgg tatctttata gtcctgtcgg 7081 gtttcgccac cactgatttg agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct 7141 atggaaaaac ggctttgccg cggccctctc acttccctgt taagtatctt cctggcatct 7201 tccaggaaat ctccgccccg ttcgtaagcc atttccgctc gccgcagtcg aacgaccgag 7261 cgtagcgagt cagtgagcga ggaagcggaa tatatcctgt atcacatatt ctgctgacgc 7321 accggtgcag ccttttttct cctgccacat gaagcacttc actgacaccc tcatcagtgc 7381 caacatagta agccagtata cactccgcta gcgctgaggt ctgcctcgtg aagaaggtgt 7441 tgctgactca taccaggcct gaatcgcccc atcatccagc cagaaagtga gggagccacg 7501 gttgatgaga gctttgttgt aggtggacca gttggtgatt ttgaactttt gctttgccac 7561 ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc ttcaactcag caaaagttcg 7621 atttattcaa caaagccacg ttgtgtctca aaatctctga tgttacattg cacaagataa 7681 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt 7741 t //