LOCUS IBMc394 5825 bp ds-DNA circular 18-NOV-2020 DEFINITION pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-14aa-FRB-T ACCESSION IBMc394 VERSION IBMc394.1 FEATURES Location/Qualifiers terminator 2312..2406 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="lambda t0 terminator" CDS 1300..1317 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="H6" CDS 1894..2172 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="FRB E coli optimized" CDS 1852..1893 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="Long Linker" promoter 2459..2556 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" CDS complement(78..956) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" CDS 2557..3216 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat" misc_feature 2237..2257 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" primer_bind 5723..5740 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS1 Fwd" misc_feature 56..77 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" primer_bind complement(2366..2382) /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS2 Rev" RBS 1276..1290 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" terminator 2184..2236 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" CDS 1318..1851 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="29-1 PACE T7 RNAP 1-179 variant: L32S, E35G, A83A, K98R, L106L, Q107K, T122S, A144T " CDS complement(3534..4682) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="trfA CDS (replication protein)" promoter 984..1267 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" ORIGIN 1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt 61 cgcggccgct tctagagtta tgacaacttg acggctacat cattcacttt ttcttcacaa 121 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 181 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 241 aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 301 aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 361 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 421 acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 481 aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 541 ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 601 aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 661 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 721 atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 781 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 841 ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 901 agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 961 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 1021 cactgcgtct tttactggcA cttctcgcta accaaaccgg taaccccgct tattaaaagc 1081 attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 1141 cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 1201 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 1261 tctccattac tagagattaa agaggagaaa TACTAGATGc atcatcacca tcaccacaac 1321 acgattaaca tcgctaagaa cgacttctct gacatcgaac tggctgctat cccgttcaac 1381 actctggctg accattacgg tgagcgtTCA gctcgcGGAc agttggccct tgagcatgag 1441 tcttacgaga tgggtgaagc acgcttccgc aagatgtttg agcgtcaact taaagctggt 1501 gaggttgcgg ataacgctgc cgccaagcct ctcatcacta ccctactccc taagatgatt 1561 GCCcgcatca acgactggtt tgaggaagtg aaagctaagc gcggcAGGcg cccgacagcc 1621 ttccagttcC TAAAAgaaat caagccggaa gccgtagcgt acatcaccat taagaccTCT 1681 ctggcttgcc taaccagtgc tgacaataca accgttcagg ctgtagcaag cgcaatcggt 1741 cggACCattg aggacgaggc tcgcttcggt cgtatccgtg accttgaagc taagcacttc 1801 aagaaaaacg ttgaggaaca actcaacaag cgcgtagggc acgtctacaa gGGTGGCTCT 1861 GGCTCGAGCG GTGGCTCTGG CTCTGGCTCG AGCGCCGTGT TATGGCACGA ACAGTGGTAT 1921 GAGGGTTTGG ATGACGCATC GCGCCAGTTC TTCGGAGAAC ATAACACCGA AAAAATGTTT 1981 GCGGCACTGG AACCTCTTTA TGAGATGCTT AAACGTGGGC CTGAAACGTT GCGTGAGATT 2041 TCTTTTCAGA ACTCATTTGG GCGTGATTTG AACGATGCCT ACGAATGGCT GATGAATTAC 2101 AAAAAGTCGA AAGATGTATC TAACCTGAAT CAAGCATGGG ATATTTACTA TAATGTCTTT 2161 CGTAAAATCG GCtaatacta gagaacgcat gagAAAGCCC CCGGAAGATC ACCTTCCGGG 2221 GGCTTTttta ttgcgctact agtagcggcc gctgcaggca tgcaagcttg cggccgcgtc 2281 gtgactggga aaaccctggc gactagtctt ggactcctgt tgatagatcc agtaatgacc 2341 tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt 2401 gagaatccag gggtccccaa taattacgat ttaaattggc gaaaatgaga cgttgatcgg 2461 cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt 2521 gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat 2581 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt 2641 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta 2701 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat 2761 gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt 2821 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa 2881 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt 2941 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat 3001 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc 3061 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg 3121 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta 3181 caacagtact gcgatgagtg gcagggcggg gcgtaatttg acttttgtcc ttttccgctg 3241 cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac 3301 gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc 3361 agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc 3421 cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccgtaggccg 3481 gccgcgatgc aggtggctgc tgaaccccca gccggaactg accccacaag gccctagcgt 3541 ttgcaatgca ccaggtcatc attgacccag gcgtgttcca ccaggccgct gcctcgcaac 3601 tcttcgcagg cttcgccgac ctgctcgcgc cacttcttca cgcgggtgga atccgatccg 3661 cacatgaggc ggaaggtttc cagcttgagc gggtacggct cccggtgcga gctgaaatag 3721 tcgaacatcc gtcgggccgt cggcgacagc ttgcggtact tctcccatat gaatttcgtg 3781 tagtggtcgc cagcaaacag cacgacgatt tcctcgtcga tcaggacctg gcaacgggac 3841 gttttcttgc cacggtccag gacgcggaag cggtgcagca gcgacaccga ttccaggtgc 3901 ccaacgcggt cggacgtgaa gcccatcgcc gtcgcctgta ggcgcgacag gcattcctcg 3961 gccttcgtgt aataccggcc attgatcgac cagcccaggt cctggcaaag ctcgtagaac 4021 gtgaaggtga tcggctcgcc gataggggtg cgcttcgcgt actccaacac ctgctgccac 4081 accagttcgt catcgtcggc ccgcagctcg acgccggtgt aggtgatctt cacgtccttg 4141 ttgacgtgga aaatgacctt gttttgcagc gcctcgcgcg ggattttctt gttgcgcgtg 4201 gtgaacaggg cagagcgggc cgtgtcgttt ggcatcgctc gcatcgtgtc cggccacggc 4261 gcaatatcga acaaggaaag ctgcatttcc ttgatctgct gcttcgtgtg tttcagcaac 4321 gcggcctgct tggcttcgct gacctgtttt gccaggtcct cgccggcggt ttttcgcttc 4381 ttggtcgtca tagttcctcg cgtgtcgatg gtcatcgact tcgccaaacc tgccgcctcc 4441 tgttcgagac gacgcgaacg ctccacggcg gccgatggcg cgggcagggc agggggagcc 4501 agttgcacgc tgtcgcgctc gatcttggcc gtagcttgct ggactatcga gccgacggac 4561 tggaaggttt cgcggggcgc acgcatgacg gtgcggcttg cgatggtttc ggcatcctcg 4621 gcggaaaacc ccgcgtcgat cagttcttgc ctgtatgcct tccggtcaaa cgtccgattc 4681 attcaccctc cttgcgggat tgccccggaa ttaattcccc ggatcgatcc gtcgatcttg 4741 atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact ttgcagggct 4801 tcccaacctt accagagggc gccccagctg gcaattccgg ttcgcttgct gtccataaaa 4861 ccgcccagtc tagctatcgc catgtaagcc cactgcaagc tacctgcttt ctctttgcgc 4921 ttgcgttttc ccttgtccag atagcccagt agctgacatt catccggggt cagcaccgtt 4981 tctgcggact ggctttctac gtggctgcca tttttggggt gaggccgttc gcggccgagg 5041 ggcgcagccc ctggggggat gggaggcccg cgttagcggg ccgggagggt tcgagaaggg 5101 ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca 5161 aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac 5221 gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat 5281 aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg cgcccctcat 5341 ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc 5401 cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc 5461 cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt 5521 gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc 5581 gcgaggtatc cacaacgccg gcggccctac atggctctgc tgtagtgagt gggttgcgct 5641 ccggcagcgg tcctgatccc ccgcagaaaa aaaggatctc aagaagatcc tttgatcttt 5701 tctacggcgc gcccagctgt ctagggcggc ggatttgtcc tactcaggag agcgttcacc 5761 gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga 5821 tgcct //