LOCUS IBMc405 5715 bp ds-DNA circular 18-NOV-2020 DEFINITION pSEVA321-PBAD(SapI-)-B30-SrpR(1-54)-KLG-M86N-T ACCESSION IBMc405 VERSION IBMc405.1 FEATURES Location/Qualifiers CDS 1297..1458 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="SrpR" primer_bind complement(2256..2272) /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS2 Rev" terminator complement(1877..1933) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" promoter 984..1267 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" CDS complement(3424..4572) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="trfA CDS (replication protein)" terminator 2202..2296 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="lambda t0 terminator" CDS 1468..1767 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86N" terminator 1779..1868 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" CDS complement(78..956) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" promoter 2349..2446 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" CDS complement(1962..2102) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" CDS 2447..3106 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat" misc_feature 57..77 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" primer_bind 5613..5630 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS1 Fwd" RBS 1276..1290 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" ORIGIN 1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt 61 cgcggccgct tctagagtta tgacaacttg acggctacat cattcacttt ttcttcacaa 121 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 181 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 241 aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 301 aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 361 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 421 acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 481 aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 541 ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 601 aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 661 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 721 atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 781 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 841 ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 901 agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 961 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 1021 cactgcgtct tttactggcA cttctcgcta accaaaccgg taaccccgct tattaaaagc 1081 attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 1141 cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 1201 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 1261 tctccattac tagagattaa agaggagaaa tactagATGG CACGTAAAAC CGCAGCAGAA 1321 GCAGAAGAAA CCCGTCAGCG TATTATTGAT GCAGCACTGG AAGTTTTTGT TGCACAGGGT 1381 GTTAGTGATG CAACCCTGGA TCAGATTGCA CGTAAAGCCG GTGTTACCCG TGGTGCAGTT 1441 TATTGGCATT TTAATGGTAA ACTTGGAtgc atctcgggag atagtttgat cagcttggcg 1501 agcacaggga aaagagttcc tattaaggat ttgttaggcg aaaaagattt tgaaatatgg 1561 gcaattaatg aacagacgat gaagctggaa tcagctaaag ttagtcgtgt attttgtacc 1621 ggcaaaaagc tagtctatac tctaaaaact cgactaggta gaactatcaa ggcaacagca 1681 aatcatagat ttttaactat tgatggttgg aaaagattag atgagctatc tttaaaagag 1741 catattgctc taccccgtaa actagaaTAA TACTAGAGtt gagaagagaa aagaaaaccg 1801 ccgatcctgt ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt 1861 tggctgaaTA CTAGAGcctt tggtcgAAAA AAAAAGCCCG CACTGTCAGG TGCGGGCTTT 1921 TTTctgtgtt tccTACTAGA GCAAGCGAGC TCGATATCAA ATTACGCCCC GCCCTGCCAC 1981 TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAAACG 2041 GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT ATAATATTTG 2101 CCCATGGGAT CGATCGATCG ATCggatcct ctagagtcga cctgcaggca tgcaagcttg 2161 cggccgcgtc gtgactggga aaaccctggc gactagtctt ggactcctgt tgatagatcc 2221 agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt 2281 ttttattggt gagaatccag gggtccccaa taattacgat ttaaattggc gaaaatgaga 2341 cgttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg 2401 cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat 2461 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt 2521 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt 2581 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg 2641 cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg 2701 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct 2761 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc 2821 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt 2881 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa 2941 cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat 3001 gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct 3061 taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttg acttttgtcc 3121 ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc 3181 tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc 3241 caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc 3301 ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg 3361 ccgtaggccg gccgcgatgc aggtggctgc tgaaccccca gccggaactg accccacaag 3421 gccctagcgt ttgcaatgca ccaggtcatc attgacccag gcgtgttcca ccaggccgct 3481 gcctcgcaac tcttcgcagg cttcgccgac ctgctcgcgc cacttcttca cgcgggtgga 3541 atccgatccg cacatgaggc ggaaggtttc cagcttgagc gggtacggct cccggtgcga 3601 gctgaaatag tcgaacatcc gtcgggccgt cggcgacagc ttgcggtact tctcccatat 3661 gaatttcgtg tagtggtcgc cagcaaacag cacgacgatt tcctcgtcga tcaggacctg 3721 gcaacgggac gttttcttgc cacggtccag gacgcggaag cggtgcagca gcgacaccga 3781 ttccaggtgc ccaacgcggt cggacgtgaa gcccatcgcc gtcgcctgta ggcgcgacag 3841 gcattcctcg gccttcgtgt aataccggcc attgatcgac cagcccaggt cctggcaaag 3901 ctcgtagaac gtgaaggtga tcggctcgcc gataggggtg cgcttcgcgt actccaacac 3961 ctgctgccac accagttcgt catcgtcggc ccgcagctcg acgccggtgt aggtgatctt 4021 cacgtccttg ttgacgtgga aaatgacctt gttttgcagc gcctcgcgcg ggattttctt 4081 gttgcgcgtg gtgaacaggg cagagcgggc cgtgtcgttt ggcatcgctc gcatcgtgtc 4141 cggccacggc gcaatatcga acaaggaaag ctgcatttcc ttgatctgct gcttcgtgtg 4201 tttcagcaac gcggcctgct tggcttcgct gacctgtttt gccaggtcct cgccggcggt 4261 ttttcgcttc ttggtcgtca tagttcctcg cgtgtcgatg gtcatcgact tcgccaaacc 4321 tgccgcctcc tgttcgagac gacgcgaacg ctccacggcg gccgatggcg cgggcagggc 4381 agggggagcc agttgcacgc tgtcgcgctc gatcttggcc gtagcttgct ggactatcga 4441 gccgacggac tggaaggttt cgcggggcgc acgcatgacg gtgcggcttg cgatggtttc 4501 ggcatcctcg gcggaaaacc ccgcgtcgat cagttcttgc ctgtatgcct tccggtcaaa 4561 cgtccgattc attcaccctc cttgcgggat tgccccggaa ttaattcccc ggatcgatcc 4621 gtcgatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact 4681 ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccgg ttcgcttgct 4741 gtccataaaa ccgcccagtc tagctatcgc catgtaagcc cactgcaagc tacctgcttt 4801 ctctttgcgc ttgcgttttc ccttgtccag atagcccagt agctgacatt catccggggt 4861 cagcaccgtt tctgcggact ggctttctac gtggctgcca tttttggggt gaggccgttc 4921 gcggccgagg ggcgcagccc ctggggggat gggaggcccg cgttagcggg ccgggagggt 4981 tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg 5041 gttaaaaaca aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg 5101 gccgaaaaac gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc 5161 aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg 5221 cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc 5281 caggcttgtc cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg 5341 cgaggctggc cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc 5401 cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc 5461 caagttttcc gcgaggtatc cacaacgccg gcggccctac atggctctgc tgtagtgagt 5521 gggttgcgct ccggcagcgg tcctgatccc ccgcagaaaa aaaggatctc aagaagatcc 5581 tttgatcttt tctacggcgc gcccagctgt ctagggcggc ggatttgtcc tactcaggag 5641 agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg 5701 ttttatttga tgcct //