LOCUS IBMc406 5778 bp ds-DNA circular 18-NOV-2020 DEFINITION pSEVA321-PBAD(SapI-)-B30-SrpR(1-75)-PDG-M86N-T ACCESSION IBMc406 VERSION IBMc406.1 FEATURES Location/Qualifiers CDS complement(3487..4635) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="trfA CDS (replication protein)" CDS complement(78..956) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" CDS 1297..1521 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="SrpR" terminator complement(1940..1996) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" terminator 2265..2359 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="lambda t0 terminator" misc_feature 57..77 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" promoter 2412..2509 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" terminator 1842..1931 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" CDS 2510..3169 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat" primer_bind 5676..5693 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS1 Fwd" CDS 1531..1830 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86N" primer_bind complement(2319..2335) /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS2 Rev" promoter 984..1267 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" RBS 1276..1290 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" CDS complement(2025..2165) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" ORIGIN 1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt 61 cgcggccgct tctagagtta tgacaacttg acggctacat cattcacttt ttcttcacaa 121 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 181 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 241 aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 301 aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 361 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 421 acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 481 aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 541 ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 601 aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 661 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 721 atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 781 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 841 ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 901 agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 961 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 1021 cactgcgtct tttactggcA cttctcgcta accaaaccgg taaccccgct tattaaaagc 1081 attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 1141 cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 1201 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 1261 tctccattac tagagattaa agaggagaaa tactagATGG CACGTAAAAC CGCAGCAGAA 1321 GCAGAAGAAA CCCGTCAGCG TATTATTGAT GCAGCACTGG AAGTTTTTGT TGCACAGGGT 1381 GTTAGTGATG CAACCCTGGA TCAGATTGCA CGTAAAGCCG GTGTTACCCG TGGTGCAGTT 1441 TATTGGCATT TTAATGGTAA ACTGGAAGTT CTGCAAGCAG TTCTGGCAAG CCGTCAGCAT 1501 CCGCTGGAAC TGGATTTTAC ACCGGATGGA tgcatctcgg gagatagttt gatcagcttg 1561 gcgagcacag ggaaaagagt tcctattaag gatttgttag gcgaaaaaga ttttgaaata 1621 tgggcaatta atgaacagac gatgaagctg gaatcagcta aagttagtcg tgtattttgt 1681 accggcaaaa agctagtcta tactctaaaa actcgactag gtagaactat caaggcaaca 1741 gcaaatcata gatttttaac tattgatggt tggaaaagat tagatgagct atctttaaaa 1801 gagcatattg ctctaccccg taaactagaa TAATACTAGA Gttgagaaga gaaaagaaaa 1861 ccgccgatcc tgtccaccgc attactgcaa ggtagtggac aagaccggcg gtcttaagtt 1921 ttttggctga aTACTAGAGc ctttggtcgA AAAAAAAAGC CCGCACTGTC AGGTGCGGGC 1981 TTTTTTctgt gtttccTACT AGAGCAAGCG AGCTCGATAT CAAATTACGC CCCGCCCTGC 2041 CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA GCCATCACAA 2101 ACGGCATGAT GAACCTGAAT CGCCAGCGGC ATCAGCACCT TGTCGCCTTG CGTATAATAT 2161 TTGCCCATGG GATCGATCGA TCGATCggat cctctagagt cgacctgcag gcatgcaagc 2221 ttgcggccgc gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga 2281 tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc 2341 gttttttatt ggtgagaatc caggggtccc caataattac gatttaaatt ggcgaaaatg 2401 agacgttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc 2461 gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa 2521 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc 2581 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt 2641 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc 2701 ccgcctgatg aatgctcatc cggaatttcg tatggcaatg aaagacggtg agctggtgat 2761 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc 2821 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt 2881 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt 2941 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga 3001 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct 3061 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat 3121 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttgacttttg 3181 tccttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca 3241 tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt 3301 atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc 3361 ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc 3421 tggccgtagg ccggccgcga tgcaggtggc tgctgaaccc ccagccggaa ctgaccccac 3481 aaggccctag cgtttgcaat gcaccaggtc atcattgacc caggcgtgtt ccaccaggcc 3541 gctgcctcgc aactcttcgc aggcttcgcc gacctgctcg cgccacttct tcacgcgggt 3601 ggaatccgat ccgcacatga ggcggaaggt ttccagcttg agcgggtacg gctcccggtg 3661 cgagctgaaa tagtcgaaca tccgtcgggc cgtcggcgac agcttgcggt acttctccca 3721 tatgaatttc gtgtagtggt cgccagcaaa cagcacgacg atttcctcgt cgatcaggac 3781 ctggcaacgg gacgttttct tgccacggtc caggacgcgg aagcggtgca gcagcgacac 3841 cgattccagg tgcccaacgc ggtcggacgt gaagcccatc gccgtcgcct gtaggcgcga 3901 caggcattcc tcggccttcg tgtaataccg gccattgatc gaccagccca ggtcctggca 3961 aagctcgtag aacgtgaagg tgatcggctc gccgataggg gtgcgcttcg cgtactccaa 4021 cacctgctgc cacaccagtt cgtcatcgtc ggcccgcagc tcgacgccgg tgtaggtgat 4081 cttcacgtcc ttgttgacgt ggaaaatgac cttgttttgc agcgcctcgc gcgggatttt 4141 cttgttgcgc gtggtgaaca gggcagagcg ggccgtgtcg tttggcatcg ctcgcatcgt 4201 gtccggccac ggcgcaatat cgaacaagga aagctgcatt tccttgatct gctgcttcgt 4261 gtgtttcagc aacgcggcct gcttggcttc gctgacctgt tttgccaggt cctcgccggc 4321 ggtttttcgc ttcttggtcg tcatagttcc tcgcgtgtcg atggtcatcg acttcgccaa 4381 acctgccgcc tcctgttcga gacgacgcga acgctccacg gcggccgatg gcgcgggcag 4441 ggcaggggga gccagttgca cgctgtcgcg ctcgatcttg gccgtagctt gctggactat 4501 cgagccgacg gactggaagg tttcgcgggg cgcacgcatg acggtgcggc ttgcgatggt 4561 ttcggcatcc tcggcggaaa accccgcgtc gatcagttct tgcctgtatg ccttccggtc 4621 aaacgtccga ttcattcacc ctccttgcgg gattgccccg gaattaattc cccggatcga 4681 tccgtcgatc ttgatcccct gcgccatcag atccttggcg gcaagaaagc catccagttt 4741 actttgcagg gcttcccaac cttaccagag ggcgccccag ctggcaattc cggttcgctt 4801 gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc 4861 tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg 4921 ggtcagcacc gtttctgcgg actggctttc tacgtggctg ccatttttgg ggtgaggccg 4981 ttcgcggccg aggggcgcag cccctggggg gatgggaggc ccgcgttagc gggccgggag 5041 ggttcgagaa gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc 5101 ctggttaaaa acaaggttta taaatattgg tttaaaagca ggttaaaaga caggttagcg 5161 gtggccgaaa aacgggcgga aacccttgca aatgctggat tttctgcctg tggacagccc 5221 ctcaaatgtc aataggtgcg cccctcatct gtcagcactc tgcccctcaa gtgtcaagga 5281 tcgcgcccct catctgtcag tagtcgcgcc cctcaagtgt caataccgca gggcacttat 5341 ccccaggctt gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat 5401 ttgcgaggct ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa 5461 cgccgcgccg ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag 5521 ggccaagttt tccgcgaggt atccacaacg ccggcggccc tacatggctc tgctgtagtg 5581 agtgggttgc gctccggcag cggtcctgat cccccgcaga aaaaaaggat ctcaagaaga 5641 tcctttgatc ttttctacgg cgcgcccagc tgtctagggc ggcggatttg tcctactcag 5701 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt 5761 tcgttttatt tgatgcct //