LOCUS IBMc407 5784 bp ds-DNA circular 18-NOV-2020 DEFINITION pSEVA321-PBAD(SapI-)-B30-SrpR(1-77)-LGG-M86N-T ACCESSION IBMc407 VERSION IBMc407.1 FEATURES Location/Qualifiers terminator 2271..2365 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="lambda t0 terminator" terminator 1848..1937 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" CDS 1537..1836 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86N" promoter 2418..2515 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" CDS 1297..1527 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="SrpR" RBS 1276..1290 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" CDS 2516..3175 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat" CDS complement(2031..2171) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" primer_bind 5682..5699 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS1 Fwd" primer_bind complement(2325..2341) /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS2 Rev" promoter 984..1267 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" terminator complement(1946..2002) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" CDS complement(3493..4641) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="trfA CDS (replication protein)" misc_feature 57..77 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" CDS complement(78..956) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" ORIGIN 1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt 61 cgcggccgct tctagagtta tgacaacttg acggctacat cattcacttt ttcttcacaa 121 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 181 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 241 aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 301 aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 361 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 421 acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 481 aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 541 ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 601 aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 661 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 721 atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 781 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 841 ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 901 agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 961 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 1021 cactgcgtct tttactggcA cttctcgcta accaaaccgg taaccccgct tattaaaagc 1081 attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 1141 cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 1201 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 1261 tctccattac tagagattaa agaggagaaa tactagATGG CACGTAAAAC CGCAGCAGAA 1321 GCAGAAGAAA CCCGTCAGCG TATTATTGAT GCAGCACTGG AAGTTTTTGT TGCACAGGGT 1381 GTTAGTGATG CAACCCTGGA TCAGATTGCA CGTAAAGCCG GTGTTACCCG TGGTGCAGTT 1441 TATTGGCATT TTAATGGTAA ACTGGAAGTT CTGCAAGCAG TTCTGGCAAG CCGTCAGCAT 1501 CCGCTGGAAC TGGATTTTAC ACCGGATCTG GGTGGAtgca tctcgggaga tagtttgatc 1561 agcttggcga gcacagggaa aagagttcct attaaggatt tgttaggcga aaaagatttt 1621 gaaatatggg caattaatga acagacgatg aagctggaat cagctaaagt tagtcgtgta 1681 ttttgtaccg gcaaaaagct agtctatact ctaaaaactc gactaggtag aactatcaag 1741 gcaacagcaa atcatagatt tttaactatt gatggttgga aaagattaga tgagctatct 1801 ttaaaagagc atattgctct accccgtaaa ctagaaTAAT ACTAGAGttg agaagagaaa 1861 agaaaaccgc cgatcctgtc caccgcatta ctgcaaggta gtggacaaga ccggcggtct 1921 taagtttttt ggctgaaTAC TAGAGccttt ggtcgAAAAA AAAAGCCCGC ACTGTCAGGT 1981 GCGGGCTTTT TTctgtgttt ccTACTAGAG CAAGCGAGCT CGATATCAAA TTACGCCCCG 2041 CCCTGCCACT CATCGCAGTA CTGTTGTAAT TCATTAAGCA TTCTGCCGAC ATGGAAGCCA 2101 TCACAAACGG CATGATGAAC CTGAATCGCC AGCGGCATCA GCACCTTGTC GCCTTGCGTA 2161 TAATATTTGC CCATGGGATC GATCGATCGA TCggatcctc tagagtcgac ctgcaggcat 2221 gcaagcttgc ggccgcgtcg tgactgggaa aaccctggcg actagtcttg gactcctgtt 2281 gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg 2341 ccgggcgttt tttattggtg agaatccagg ggtccccaat aattacgatt taaattggcg 2401 aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc 2461 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga 2521 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt 2581 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac 2641 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat 2701 tcttgcccgc ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct 2761 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt 2821 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca 2881 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat 2941 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa 3001 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa 3061 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg 3121 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaatttga 3181 cttttgtcct tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta 3241 tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct 3301 tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg 3361 tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg 3421 cgaggctggc cgtaggccgg ccgcgatgca ggtggctgct gaacccccag ccggaactga 3481 ccccacaagg ccctagcgtt tgcaatgcac caggtcatca ttgacccagg cgtgttccac 3541 caggccgctg cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac 3601 gcgggtggaa tccgatccgc acatgaggcg gaaggtttcc agcttgagcg ggtacggctc 3661 ccggtgcgag ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct tgcggtactt 3721 ctcccatatg aatttcgtgt agtggtcgcc agcaaacagc acgacgattt cctcgtcgat 3781 caggacctgg caacgggacg ttttcttgcc acggtccagg acgcggaagc ggtgcagcag 3841 cgacaccgat tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag 3901 gcgcgacagg cattcctcgg ccttcgtgta ataccggcca ttgatcgacc agcccaggtc 3961 ctggcaaagc tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta 4021 ctccaacacc tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta 4081 ggtgatcttc acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg 4141 gattttcttg ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg 4201 catcgtgtcc ggccacggcg caatatcgaa caaggaaagc tgcatttcct tgatctgctg 4261 cttcgtgtgt ttcagcaacg cggcctgctt ggcttcgctg acctgttttg ccaggtcctc 4321 gccggcggtt tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt 4381 cgccaaacct gccgcctcct gttcgagacg acgcgaacgc tccacggcgg ccgatggcgc 4441 gggcagggca gggggagcca gttgcacgct gtcgcgctcg atcttggccg tagcttgctg 4501 gactatcgag ccgacggact ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc 4561 gatggtttcg gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt 4621 ccggtcaaac gtccgattca ttcaccctcc ttgcgggatt gccccggaat taattccccg 4681 gatcgatccg tcgatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc 4741 cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt 4801 tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct 4861 acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc 4921 atccggggtc agcaccgttt ctgcggactg gctttctacg tggctgccat ttttggggtg 4981 aggccgttcg cggccgaggg gcgcagcccc tggggggatg ggaggcccgc gttagcgggc 5041 cgggagggtt cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc 5101 gcagccctgg ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg 5161 ttagcggtgg ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga 5221 cagcccctca aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt 5281 caaggatcgc gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc 5341 acttatcccc aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc 5401 gccgatttgc gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc 5461 tgtcaacgcc gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc 5521 agtgagggcc aagttttccg cgaggtatcc acaacgccgg cggccctaca tggctctgct 5581 gtagtgagtg ggttgcgctc cggcagcggt cctgatcccc cgcagaaaaa aaggatctca 5641 agaagatcct ttgatctttt ctacggcgcg cccagctgtc tagggcggcg gatttgtcct 5701 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg 5761 agcctttcgt tttatttgat gcct //