LOCUS IBMc408 5880 bp ds-DNA circular 18-NOV-2020 DEFINITION pSEVA321-PBAD(SapI-)-B30-SrpR(1-109)-QGG-M86N-T ACCESSION IBMc408 VERSION IBMc408.1 FEATURES Location/Qualifiers terminator 1944..2033 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" CDS 1297..1623 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="SrpR" promoter 2514..2611 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" promoter 984..1267 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" CDS 2612..3271 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat" primer_bind 5778..5795 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS1 Fwd" CDS 1633..1932 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86N" primer_bind complement(2421..2437) /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS2 Rev" CDS complement(78..956) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" terminator complement(2042..2098) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" CDS complement(2127..2267) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" CDS complement(3589..4737) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="trfA CDS (replication protein)" misc_feature 57..77 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" terminator 2367..2461 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="lambda t0 terminator" RBS 1276..1290 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" ORIGIN 1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt 61 cgcggccgct tctagagtta tgacaacttg acggctacat cattcacttt ttcttcacaa 121 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 181 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 241 aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 301 aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 361 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 421 acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 481 aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 541 ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 601 aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 661 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 721 atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 781 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 841 ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 901 agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 961 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 1021 cactgcgtct tttactggcA cttctcgcta accaaaccgg taaccccgct tattaaaagc 1081 attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 1141 cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 1201 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 1261 tctccattac tagagattaa agaggagaaa tactagATGG CACGTAAAAC CGCAGCAGAA 1321 GCAGAAGAAA CCCGTCAGCG TATTATTGAT GCAGCACTGG AAGTTTTTGT TGCACAGGGT 1381 GTTAGTGATG CAACCCTGGA TCAGATTGCA CGTAAAGCCG GTGTTACCCG TGGTGCAGTT 1441 TATTGGCATT TTAATGGTAA ACTGGAAGTT CTGCAAGCAG TTCTGGCAAG CCGTCAGCAT 1501 CCGCTGGAAC TGGATTTTAC ACCGGATCTG GGTATTGAAC GTAGCTGGGA AGCAGTTGTT 1561 GTTGCAATGC TGGATGCAGT TCATAGTCCG CAGAGCAAAC AGTTTAGCGA AATTCTGATT 1621 TATCAGGGTG GAtgcatctc gggagatagt ttgatcagct tggcgagcac agggaaaaga 1681 gttcctatta aggatttgtt aggcgaaaaa gattttgaaa tatgggcaat taatgaacag 1741 acgatgaagc tggaatcagc taaagttagt cgtgtatttt gtaccggcaa aaagctagtc 1801 tatactctaa aaactcgact aggtagaact atcaaggcaa cagcaaatca tagattttta 1861 actattgatg gttggaaaag attagatgag ctatctttaa aagagcatat tgctctaccc 1921 cgtaaactag aaTAATACTA GAGttgagaa gagaaaagaa aaccgccgat cctgtccacc 1981 gcattactgc aaggtagtgg acaagaccgg cggtcttaag ttttttggct gaaTACTAGA 2041 Gcctttggtc gAAAAAAAAA GCCCGCACTG TCAGGTGCGG GCTTTTTTct gtgtttccTA 2101 CTAGAGCAAG CGAGCTCGAT ATCAAATTAC GCCCCGCCCT GCCACTCATC GCAGTACTGT 2161 TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC AAACGGCATG ATGAACCTGA 2221 ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT ATTTGCCCAT GGGATCGATC 2281 GATCGATCgg atcctctaga gtcgacctgc aggcatgcaa gcttgcggcc gcgtcgtgac 2341 tgggaaaacc ctggcgacta gtcttggact cctgttgata gatccagtaa tgacctcaga 2401 actccatctg gatttgttca gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa 2461 tccaggggtc cccaataatt acgatttaaa ttggcgaaaa tgagacgttg atcggcacgt 2521 aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt 2581 atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac 2641 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca 2701 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa 2761 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca 2821 tccggaattt cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc 2881 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca 2941 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa 3001 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg 3061 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt 3121 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca 3181 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca 3241 gtactgcgat gagtggcagg gcggggcgta atttgacttt tgtccttttc cgctgcataa 3301 ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat 3361 acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg 3421 ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat 3481 tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccgta ggccggccgc 3541 gatgcaggtg gctgctgaac ccccagccgg aactgacccc acaaggccct agcgtttgca 3601 atgcaccagg tcatcattga cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc 3661 gcaggcttcg ccgacctgct cgcgccactt cttcacgcgg gtggaatccg atccgcacat 3721 gaggcggaag gtttccagct tgagcgggta cggctcccgg tgcgagctga aatagtcgaa 3781 catccgtcgg gccgtcggcg acagcttgcg gtacttctcc catatgaatt tcgtgtagtg 3841 gtcgccagca aacagcacga cgatttcctc gtcgatcagg acctggcaac gggacgtttt 3901 cttgccacgg tccaggacgc ggaagcggtg cagcagcgac accgattcca ggtgcccaac 3961 gcggtcggac gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt 4021 cgtgtaatac cggccattga tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa 4081 ggtgatcggc tcgccgatag gggtgcgctt cgcgtactcc aacacctgct gccacaccag 4141 ttcgtcatcg tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac 4201 gtggaaaatg accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa 4261 cagggcagag cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat 4321 atcgaacaag gaaagctgca tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc 4381 ctgcttggct tcgctgacct gttttgccag gtcctcgccg gcggtttttc gcttcttggt 4441 cgtcatagtt cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg cctcctgttc 4501 gagacgacgc gaacgctcca cggcggccga tggcgcgggc agggcagggg gagccagttg 4561 cacgctgtcg cgctcgatct tggccgtagc ttgctggact atcgagccga cggactggaa 4621 ggtttcgcgg ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga 4681 aaaccccgcg tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc gattcattca 4741 ccctccttgc gggattgccc cggaattaat tccccggatc gatccgtcga tcttgatccc 4801 ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca 4861 accttaccag agggcgcccc agctggcaat tccggttcgc ttgctgtcca taaaaccgcc 4921 cagtctagct atcgccatgt aagcccactg caagctacct gctttctctt tgcgcttgcg 4981 ttttcccttg tccagatagc ccagtagctg acattcatcc ggggtcagca ccgtttctgc 5041 ggactggctt tctacgtggc tgccattttt ggggtgaggc cgttcgcggc cgaggggcgc 5101 agcccctggg gggatgggag gcccgcgtta gcgggccggg agggttcgag aagggggggc 5161 accccccttc ggcgtgcgcg gtcacgcgca cagggcgcag ccctggttaa aaacaaggtt 5221 tataaatatt ggtttaaaag caggttaaaa gacaggttag cggtggccga aaaacgggcg 5281 gaaacccttg caaatgctgg attttctgcc tgtggacagc ccctcaaatg tcaataggtg 5341 cgcccctcat ctgtcagcac tctgcccctc aagtgtcaag gatcgcgccc ctcatctgtc 5401 agtagtcgcg cccctcaagt gtcaataccg cagggcactt atccccaggc ttgtccacat 5461 catctgtggg aaactcgcgt aaaatcaggc gttttcgccg atttgcgagg ctggccagct 5521 ccacgtcgcc ggccgaaatc gagcctgccc ctcatctgtc aacgccgcgc cgggtgagtc 5581 ggcccctcaa gtgtcaacgt ccgcccctca tctgtcagtg agggccaagt tttccgcgag 5641 gtatccacaa cgccggcggc cctacatggc tctgctgtag tgagtgggtt gcgctccggc 5701 agcggtcctg atcccccgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 5761 ggcgcgccca gctgtctagg gcggcggatt tgtcctactc aggagagcgt tcaccgacaa 5821 acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct //