LOCUS IBMc410 5895 bp ds-DNA circular 18-NOV-2020 DEFINITION pSEVA321-PBAD(SapI-)-B30-SrpR(1-114)-SGG-M86N-T ACCESSION IBMc410 VERSION IBMc410.1 FEATURES Location/Qualifiers primer_bind 5793..5810 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS1 Fwd" misc_feature 57..77 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" primer_bind complement(2436..2452) /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS2 Rev" CDS complement(78..956) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" CDS 1297..1638 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="SrpR" promoter 984..1267 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" CDS complement(3604..4752) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="trfA CDS (replication protein)" CDS complement(2142..2282) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" terminator 2382..2476 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="lambda t0 terminator" terminator complement(2057..2113) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" RBS 1276..1290 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" terminator 1959..2048 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" promoter 2529..2626 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" CDS 2627..3286 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat" CDS 1648..1947 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86N" ORIGIN 1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt 61 cgcggccgct tctagagtta tgacaacttg acggctacat cattcacttt ttcttcacaa 121 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 181 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 241 aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 301 aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 361 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 421 acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 481 aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 541 ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 601 aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 661 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 721 atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 781 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 841 ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 901 agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 961 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 1021 cactgcgtct tttactggcA cttctcgcta accaaaccgg taaccccgct tattaaaagc 1081 attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 1141 cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 1201 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 1261 tctccattac tagagattaa agaggagaaa tactagATGG CACGTAAAAC CGCAGCAGAA 1321 GCAGAAGAAA CCCGTCAGCG TATTATTGAT GCAGCACTGG AAGTTTTTGT TGCACAGGGT 1381 GTTAGTGATG CAACCCTGGA TCAGATTGCA CGTAAAGCCG GTGTTACCCG TGGTGCAGTT 1441 TATTGGCATT TTAATGGTAA ACTGGAAGTT CTGCAAGCAG TTCTGGCAAG CCGTCAGCAT 1501 CCGCTGGAAC TGGATTTTAC ACCGGATCTG GGTATTGAAC GTAGCTGGGA AGCAGTTGTT 1561 GTTGCAATGC TGGATGCAGT TCATAGTCCG CAGAGCAAAC AGTTTAGCGA AATTCTGATT 1621 TATCAGGGTC TGGATGAAAG CGGTGGAtgc atctcgggag atagtttgat cagcttggcg 1681 agcacaggga aaagagttcc tattaaggat ttgttaggcg aaaaagattt tgaaatatgg 1741 gcaattaatg aacagacgat gaagctggaa tcagctaaag ttagtcgtgt attttgtacc 1801 ggcaaaaagc tagtctatac tctaaaaact cgactaggta gaactatcaa ggcaacagca 1861 aatcatagat ttttaactat tgatggttgg aaaagattag atgagctatc tttaaaagag 1921 catattgctc taccccgtaa actagaaTAA TACTAGAGtt gagaagagaa aagaaaaccg 1981 ccgatcctgt ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt 2041 tggctgaaTA CTAGAGcctt tggtcgAAAA AAAAAGCCCG CACTGTCAGG TGCGGGCTTT 2101 TTTctgtgtt tccTACTAGA GCAAGCGAGC TCGATATCAA ATTACGCCCC GCCCTGCCAC 2161 TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAAACG 2221 GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT ATAATATTTG 2281 CCCATGGGAT CGATCGATCG ATCggatcct ctagagtcga cctgcaggca tgcaagcttg 2341 cggccgcgtc gtgactggga aaaccctggc gactagtctt ggactcctgt tgatagatcc 2401 agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt 2461 ttttattggt gagaatccag gggtccccaa taattacgat ttaaattggc gaaaatgaga 2521 cgttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg 2581 cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat 2641 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt 2701 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt 2761 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg 2821 cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg 2881 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct 2941 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc 3001 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt 3061 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa 3121 cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat 3181 gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct 3241 taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttg acttttgtcc 3301 ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc 3361 tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc 3421 caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc 3481 ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg 3541 ccgtaggccg gccgcgatgc aggtggctgc tgaaccccca gccggaactg accccacaag 3601 gccctagcgt ttgcaatgca ccaggtcatc attgacccag gcgtgttcca ccaggccgct 3661 gcctcgcaac tcttcgcagg cttcgccgac ctgctcgcgc cacttcttca cgcgggtgga 3721 atccgatccg cacatgaggc ggaaggtttc cagcttgagc gggtacggct cccggtgcga 3781 gctgaaatag tcgaacatcc gtcgggccgt cggcgacagc ttgcggtact tctcccatat 3841 gaatttcgtg tagtggtcgc cagcaaacag cacgacgatt tcctcgtcga tcaggacctg 3901 gcaacgggac gttttcttgc cacggtccag gacgcggaag cggtgcagca gcgacaccga 3961 ttccaggtgc ccaacgcggt cggacgtgaa gcccatcgcc gtcgcctgta ggcgcgacag 4021 gcattcctcg gccttcgtgt aataccggcc attgatcgac cagcccaggt cctggcaaag 4081 ctcgtagaac gtgaaggtga tcggctcgcc gataggggtg cgcttcgcgt actccaacac 4141 ctgctgccac accagttcgt catcgtcggc ccgcagctcg acgccggtgt aggtgatctt 4201 cacgtccttg ttgacgtgga aaatgacctt gttttgcagc gcctcgcgcg ggattttctt 4261 gttgcgcgtg gtgaacaggg cagagcgggc cgtgtcgttt ggcatcgctc gcatcgtgtc 4321 cggccacggc gcaatatcga acaaggaaag ctgcatttcc ttgatctgct gcttcgtgtg 4381 tttcagcaac gcggcctgct tggcttcgct gacctgtttt gccaggtcct cgccggcggt 4441 ttttcgcttc ttggtcgtca tagttcctcg cgtgtcgatg gtcatcgact tcgccaaacc 4501 tgccgcctcc tgttcgagac gacgcgaacg ctccacggcg gccgatggcg cgggcagggc 4561 agggggagcc agttgcacgc tgtcgcgctc gatcttggcc gtagcttgct ggactatcga 4621 gccgacggac tggaaggttt cgcggggcgc acgcatgacg gtgcggcttg cgatggtttc 4681 ggcatcctcg gcggaaaacc ccgcgtcgat cagttcttgc ctgtatgcct tccggtcaaa 4741 cgtccgattc attcaccctc cttgcgggat tgccccggaa ttaattcccc ggatcgatcc 4801 gtcgatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact 4861 ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccgg ttcgcttgct 4921 gtccataaaa ccgcccagtc tagctatcgc catgtaagcc cactgcaagc tacctgcttt 4981 ctctttgcgc ttgcgttttc ccttgtccag atagcccagt agctgacatt catccggggt 5041 cagcaccgtt tctgcggact ggctttctac gtggctgcca tttttggggt gaggccgttc 5101 gcggccgagg ggcgcagccc ctggggggat gggaggcccg cgttagcggg ccgggagggt 5161 tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg 5221 gttaaaaaca aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg 5281 gccgaaaaac gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc 5341 aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg 5401 cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc 5461 caggcttgtc cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg 5521 cgaggctggc cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc 5581 cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc 5641 caagttttcc gcgaggtatc cacaacgccg gcggccctac atggctctgc tgtagtgagt 5701 gggttgcgct ccggcagcgg tcctgatccc ccgcagaaaa aaaggatctc aagaagatcc 5761 tttgatcttt tctacggcgc gcccagctgt ctagggcggc ggatttgtcc tactcaggag 5821 agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg 5881 ttttatttga tgcct //