LOCUS IBMc411 5901 bp ds-DNA circular 18-NOV-2020 DEFINITION pSEVA321-PBAD(SapI-)-B30-SrpR(1-116)-LIG-M86N-T ACCESSION IBMc411 VERSION IBMc411.1 FEATURES Location/Qualifiers primer_bind complement(2442..2458) /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS2 Rev" CDS 1297..1644 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="SrpR" terminator complement(2063..2119) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" CDS complement(78..956) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" CDS complement(3610..4758) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="trfA CDS (replication protein)" promoter 984..1267 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" terminator 2388..2482 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="lambda t0 terminator" CDS 1654..1953 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86N" terminator 1965..2054 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" promoter 2535..2632 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" CDS complement(2148..2288) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" CDS 2633..3292 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat" misc_feature 57..77 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" primer_bind 5799..5816 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS1 Fwd" RBS 1276..1290 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" ORIGIN 1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt 61 cgcggccgct tctagagtta tgacaacttg acggctacat cattcacttt ttcttcacaa 121 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 181 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 241 aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 301 aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 361 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 421 acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 481 aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 541 ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 601 aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 661 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 721 atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 781 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 841 ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 901 agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 961 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 1021 cactgcgtct tttactggcA cttctcgcta accaaaccgg taaccccgct tattaaaagc 1081 attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 1141 cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 1201 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 1261 tctccattac tagagattaa agaggagaaa tactagATGG CACGTAAAAC CGCAGCAGAA 1321 GCAGAAGAAA CCCGTCAGCG TATTATTGAT GCAGCACTGG AAGTTTTTGT TGCACAGGGT 1381 GTTAGTGATG CAACCCTGGA TCAGATTGCA CGTAAAGCCG GTGTTACCCG TGGTGCAGTT 1441 TATTGGCATT TTAATGGTAA ACTGGAAGTT CTGCAAGCAG TTCTGGCAAG CCGTCAGCAT 1501 CCGCTGGAAC TGGATTTTAC ACCGGATCTG GGTATTGAAC GTAGCTGGGA AGCAGTTGTT 1561 GTTGCAATGC TGGATGCAGT TCATAGTCCG CAGAGCAAAC AGTTTAGCGA AATTCTGATT 1621 TATCAGGGTC TGGATGAAAG CGGTCTGATT GGAtgcatct cgggagatag tttgatcagc 1681 ttggcgagca cagggaaaag agttcctatt aaggatttgt taggcgaaaa agattttgaa 1741 atatgggcaa ttaatgaaca gacgatgaag ctggaatcag ctaaagttag tcgtgtattt 1801 tgtaccggca aaaagctagt ctatactcta aaaactcgac taggtagaac tatcaaggca 1861 acagcaaatc atagattttt aactattgat ggttggaaaa gattagatga gctatcttta 1921 aaagagcata ttgctctacc ccgtaaacta gaaTAATACT AGAGttgaga agagaaaaga 1981 aaaccgccga tcctgtccac cgcattactg caaggtagtg gacaagaccg gcggtcttaa 2041 gttttttggc tgaaTACTAG AGcctttggt cgAAAAAAAA AGCCCGCACT GTCAGGTGCG 2101 GGCTTTTTTc tgtgtttccT ACTAGAGCAA GCGAGCTCGA TATCAAATTA CGCCCCGCCC 2161 TGCCACTCAT CGCAGTACTG TTGTAATTCA TTAAGCATTC TGCCGACATG GAAGCCATCA 2221 CAAACGGCAT GATGAACCTG AATCGCCAGC GGCATCAGCA CCTTGTCGCC TTGCGTATAA 2281 TATTTGCCCA TGGGATCGAT CGATCGATCg gatcctctag agtcgacctg caggcatgca 2341 agcttgcggc cgcgtcgtga ctgggaaaac cctggcgact agtcttggac tcctgttgat 2401 agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg 2461 ggcgtttttt attggtgaga atccaggggt ccccaataat tacgatttaa attggcgaaa 2521 atgagacgtt gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact 2581 accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa 2641 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga 2701 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc 2761 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct 2821 tgcccgcctg atgaatgctc atccggaatt tcgtatggca atgaaagacg gtgagctggt 2881 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc 2941 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga 3001 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt 3061 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat 3121 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt 3181 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag 3241 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aatttgactt 3301 ttgtcctttt ccgctgcata accctgcttc ggggtcatta tagcgatttt ttcggtatat 3361 ccatcctttt tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg 3421 tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc gagcgggtgt 3481 tccttcttca ctgtccctta ttcgcacctg gcggtgctca acgggaatcc tgctctgcga 3541 ggctggccgt aggccggccg cgatgcaggt ggctgctgaa cccccagccg gaactgaccc 3601 cacaaggccc tagcgtttgc aatgcaccag gtcatcattg acccaggcgt gttccaccag 3661 gccgctgcct cgcaactctt cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg 3721 ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt acggctcccg 3781 gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc gacagcttgc ggtacttctc 3841 ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct cgtcgatcag 3901 gacctggcaa cgggacgttt tcttgccacg gtccaggacg cggaagcggt gcagcagcga 3961 caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg 4021 cgacaggcat tcctcggcct tcgtgtaata ccggccattg atcgaccagc ccaggtcctg 4081 gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc 4141 caacacctgc tgccacacca gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt 4201 gatcttcacg tccttgttga cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat 4261 tttcttgttg cgcgtggtga acagggcaga gcgggccgtg tcgtttggca tcgctcgcat 4321 cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc atttccttga tctgctgctt 4381 cgtgtgtttc agcaacgcgg cctgcttggc ttcgctgacc tgttttgcca ggtcctcgcc 4441 ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc 4501 caaacctgcc gcctcctgtt cgagacgacg cgaacgctcc acggcggccg atggcgcggg 4561 cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag cttgctggac 4621 tatcgagccg acggactgga aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat 4681 ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg 4741 gtcaaacgtc cgattcattc accctccttg cgggattgcc ccggaattaa ttccccggat 4801 cgatccgtcg atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag 4861 tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg 4921 cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc 4981 tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc 5041 cggggtcagc accgtttctg cggactggct ttctacgtgg ctgccatttt tggggtgagg 5101 ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt agcgggccgg 5161 gagggttcga gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca 5221 gccctggtta aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa agacaggtta 5281 gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc ctgtggacag 5341 cccctcaaat gtcaataggt gcgcccctca tctgtcagca ctctgcccct caagtgtcaa 5401 ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc gcagggcact 5461 tatccccagg cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc 5521 gatttgcgag gctggccagc tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt 5581 caacgccgcg ccgggtgagt cggcccctca agtgtcaacg tccgcccctc atctgtcagt 5641 gagggccaag ttttccgcga ggtatccaca acgccggcgg ccctacatgg ctctgctgta 5701 gtgagtgggt tgcgctccgg cagcggtcct gatcccccgc agaaaaaaag gatctcaaga 5761 agatcctttg atcttttcta cggcgcgccc agctgtctag ggcggcggat ttgtcctact 5821 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc 5881 ctttcgtttt atttgatgcc t //