LOCUS IBMc412 6060 bp ds-DNA circular 18-NOV-2020 DEFINITION pSEVA321-PBAD(SapI-)-B30-SrpR(1-169)-GLG-M86N-T ACCESSION IBMc412 VERSION IBMc412.1 FEATURES Location/Qualifiers CDS 1297..1803 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="SrpR" terminator complement(2222..2278) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" CDS complement(3769..4917) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="trfA CDS (replication protein)" RBS 1276..1290 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" terminator 2547..2641 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="lambda t0 terminator" CDS complement(78..956) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" terminator 2124..2213 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" promoter 984..1267 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" promoter 2694..2791 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" misc_feature 57..77 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" CDS 2792..3451 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat" CDS 1813..2112 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86N" primer_bind 5958..5975 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS1 Fwd" primer_bind complement(2601..2617) /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS2 Rev" CDS complement(2307..2447) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" ORIGIN 1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt 61 cgcggccgct tctagagtta tgacaacttg acggctacat cattcacttt ttcttcacaa 121 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 181 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 241 aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 301 aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 361 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 421 acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 481 aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 541 ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 601 aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 661 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 721 atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 781 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 841 ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 901 agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 961 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 1021 cactgcgtct tttactggcA cttctcgcta accaaaccgg taaccccgct tattaaaagc 1081 attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 1141 cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 1201 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 1261 tctccattac tagagattaa agaggagaaa tactagATGG CACGTAAAAC CGCAGCAGAA 1321 GCAGAAGAAA CCCGTCAGCG TATTATTGAT GCAGCACTGG AAGTTTTTGT TGCACAGGGT 1381 GTTAGTGATG CAACCCTGGA TCAGATTGCA CGTAAAGCCG GTGTTACCCG TGGTGCAGTT 1441 TATTGGCATT TTAATGGTAA ACTGGAAGTT CTGCAAGCAG TTCTGGCAAG CCGTCAGCAT 1501 CCGCTGGAAC TGGATTTTAC ACCGGATCTG GGTATTGAAC GTAGCTGGGA AGCAGTTGTT 1561 GTTGCAATGC TGGATGCAGT TCATAGTCCG CAGAGCAAAC AGTTTAGCGA AATTCTGATT 1621 TATCAGGGTC TGGATGAAAG CGGTCTGATT CATAATCGTA TGGTTCAGGC AAGCGATCGT 1681 TTTCTGCAAT ATATTCATCA GGTTCTGCGT CATGCAGTTA CCCAGGGTGA ACTGCCGATT 1741 AATCTGGATC TGCAAACCAG CATTGGTGTT TTTAAAGGTC TGATTACCGG TCTGCTGTAT 1801 GAAGGTCTTG GAtgcatctc gggagatagt ttgatcagct tggcgagcac agggaaaaga 1861 gttcctatta aggatttgtt aggcgaaaaa gattttgaaa tatgggcaat taatgaacag 1921 acgatgaagc tggaatcagc taaagttagt cgtgtatttt gtaccggcaa aaagctagtc 1981 tatactctaa aaactcgact aggtagaact atcaaggcaa cagcaaatca tagattttta 2041 actattgatg gttggaaaag attagatgag ctatctttaa aagagcatat tgctctaccc 2101 cgtaaactag aaTAATACTA GAGttgagaa gagaaaagaa aaccgccgat cctgtccacc 2161 gcattactgc aaggtagtgg acaagaccgg cggtcttaag ttttttggct gaaTACTAGA 2221 Gcctttggtc gAAAAAAAAA GCCCGCACTG TCAGGTGCGG GCTTTTTTct gtgtttccTA 2281 CTAGAGCAAG CGAGCTCGAT ATCAAATTAC GCCCCGCCCT GCCACTCATC GCAGTACTGT 2341 TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC AAACGGCATG ATGAACCTGA 2401 ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT ATTTGCCCAT GGGATCGATC 2461 GATCGATCgg atcctctaga gtcgacctgc aggcatgcaa gcttgcggcc gcgtcgtgac 2521 tgggaaaacc ctggcgacta gtcttggact cctgttgata gatccagtaa tgacctcaga 2581 actccatctg gatttgttca gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa 2641 tccaggggtc cccaataatt acgatttaaa ttggcgaaaa tgagacgttg atcggcacgt 2701 aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt 2761 atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac 2821 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca 2881 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa 2941 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca 3001 tccggaattt cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc 3061 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca 3121 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa 3181 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg 3241 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt 3301 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca 3361 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca 3421 gtactgcgat gagtggcagg gcggggcgta atttgacttt tgtccttttc cgctgcataa 3481 ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat 3541 acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg 3601 ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat 3661 tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccgta ggccggccgc 3721 gatgcaggtg gctgctgaac ccccagccgg aactgacccc acaaggccct agcgtttgca 3781 atgcaccagg tcatcattga cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc 3841 gcaggcttcg ccgacctgct cgcgccactt cttcacgcgg gtggaatccg atccgcacat 3901 gaggcggaag gtttccagct tgagcgggta cggctcccgg tgcgagctga aatagtcgaa 3961 catccgtcgg gccgtcggcg acagcttgcg gtacttctcc catatgaatt tcgtgtagtg 4021 gtcgccagca aacagcacga cgatttcctc gtcgatcagg acctggcaac gggacgtttt 4081 cttgccacgg tccaggacgc ggaagcggtg cagcagcgac accgattcca ggtgcccaac 4141 gcggtcggac gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt 4201 cgtgtaatac cggccattga tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa 4261 ggtgatcggc tcgccgatag gggtgcgctt cgcgtactcc aacacctgct gccacaccag 4321 ttcgtcatcg tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac 4381 gtggaaaatg accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa 4441 cagggcagag cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat 4501 atcgaacaag gaaagctgca tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc 4561 ctgcttggct tcgctgacct gttttgccag gtcctcgccg gcggtttttc gcttcttggt 4621 cgtcatagtt cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg cctcctgttc 4681 gagacgacgc gaacgctcca cggcggccga tggcgcgggc agggcagggg gagccagttg 4741 cacgctgtcg cgctcgatct tggccgtagc ttgctggact atcgagccga cggactggaa 4801 ggtttcgcgg ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga 4861 aaaccccgcg tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc gattcattca 4921 ccctccttgc gggattgccc cggaattaat tccccggatc gatccgtcga tcttgatccc 4981 ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca 5041 accttaccag agggcgcccc agctggcaat tccggttcgc ttgctgtcca taaaaccgcc 5101 cagtctagct atcgccatgt aagcccactg caagctacct gctttctctt tgcgcttgcg 5161 ttttcccttg tccagatagc ccagtagctg acattcatcc ggggtcagca ccgtttctgc 5221 ggactggctt tctacgtggc tgccattttt ggggtgaggc cgttcgcggc cgaggggcgc 5281 agcccctggg gggatgggag gcccgcgtta gcgggccggg agggttcgag aagggggggc 5341 accccccttc ggcgtgcgcg gtcacgcgca cagggcgcag ccctggttaa aaacaaggtt 5401 tataaatatt ggtttaaaag caggttaaaa gacaggttag cggtggccga aaaacgggcg 5461 gaaacccttg caaatgctgg attttctgcc tgtggacagc ccctcaaatg tcaataggtg 5521 cgcccctcat ctgtcagcac tctgcccctc aagtgtcaag gatcgcgccc ctcatctgtc 5581 agtagtcgcg cccctcaagt gtcaataccg cagggcactt atccccaggc ttgtccacat 5641 catctgtggg aaactcgcgt aaaatcaggc gttttcgccg atttgcgagg ctggccagct 5701 ccacgtcgcc ggccgaaatc gagcctgccc ctcatctgtc aacgccgcgc cgggtgagtc 5761 ggcccctcaa gtgtcaacgt ccgcccctca tctgtcagtg agggccaagt tttccgcgag 5821 gtatccacaa cgccggcggc cctacatggc tctgctgtag tgagtgggtt gcgctccggc 5881 agcggtcctg atcccccgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 5941 ggcgcgccca gctgtctagg gcggcggatt tgtcctactc aggagagcgt tcaccgacaa 6001 acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct //