LOCUS IBMc413 6063 bp ds-DNA circular 18-NOV-2020 DEFINITION pSEVA321-PBAD(SapI-)-B30-SrpR(1-170)-LRG-M86N-T ACCESSION IBMc413 VERSION IBMc413.1 FEATURES Location/Qualifiers misc_feature 57..77 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" CDS 1297..1806 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="SrpR" terminator 2550..2644 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="lambda t0 terminator" terminator complement(2225..2281) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" CDS complement(2310..2450) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" terminator 2127..2216 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" promoter 2697..2794 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" CDS 2795..3454 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat" RBS 1276..1290 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" primer_bind 5961..5978 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS1 Fwd" CDS 1816..2115 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86N" promoter 984..1267 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" primer_bind complement(2604..2620) /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS2 Rev" CDS complement(3772..4920) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="trfA CDS (replication protein)" CDS complement(78..956) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" ORIGIN 1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt 61 cgcggccgct tctagagtta tgacaacttg acggctacat cattcacttt ttcttcacaa 121 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 181 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 241 aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 301 aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 361 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 421 acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 481 aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 541 ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 601 aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 661 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 721 atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 781 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 841 ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 901 agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 961 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 1021 cactgcgtct tttactggcA cttctcgcta accaaaccgg taaccccgct tattaaaagc 1081 attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 1141 cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 1201 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 1261 tctccattac tagagattaa agaggagaaa tactagATGG CACGTAAAAC CGCAGCAGAA 1321 GCAGAAGAAA CCCGTCAGCG TATTATTGAT GCAGCACTGG AAGTTTTTGT TGCACAGGGT 1381 GTTAGTGATG CAACCCTGGA TCAGATTGCA CGTAAAGCCG GTGTTACCCG TGGTGCAGTT 1441 TATTGGCATT TTAATGGTAA ACTGGAAGTT CTGCAAGCAG TTCTGGCAAG CCGTCAGCAT 1501 CCGCTGGAAC TGGATTTTAC ACCGGATCTG GGTATTGAAC GTAGCTGGGA AGCAGTTGTT 1561 GTTGCAATGC TGGATGCAGT TCATAGTCCG CAGAGCAAAC AGTTTAGCGA AATTCTGATT 1621 TATCAGGGTC TGGATGAAAG CGGTCTGATT CATAATCGTA TGGTTCAGGC AAGCGATCGT 1681 TTTCTGCAAT ATATTCATCA GGTTCTGCGT CATGCAGTTA CCCAGGGTGA ACTGCCGATT 1741 AATCTGGATC TGCAAACCAG CATTGGTGTT TTTAAAGGTC TGATTACCGG TCTGCTGTAT 1801 GAAGGTCTGC GTGGAtgcat ctcgggagat agtttgatca gcttggcgag cacagggaaa 1861 agagttccta ttaaggattt gttaggcgaa aaagattttg aaatatgggc aattaatgaa 1921 cagacgatga agctggaatc agctaaagtt agtcgtgtat tttgtaccgg caaaaagcta 1981 gtctatactc taaaaactcg actaggtaga actatcaagg caacagcaaa tcatagattt 2041 ttaactattg atggttggaa aagattagat gagctatctt taaaagagca tattgctcta 2101 ccccgtaaac tagaaTAATA CTAGAGttga gaagagaaaa gaaaaccgcc gatcctgtcc 2161 accgcattac tgcaaggtag tggacaagac cggcggtctt aagttttttg gctgaaTACT 2221 AGAGcctttg gtcgAAAAAA AAAGCCCGCA CTGTCAGGTG CGGGCTTTTT Tctgtgtttc 2281 cTACTAGAGC AAGCGAGCTC GATATCAAAT TACGCCCCGC CCTGCCACTC ATCGCAGTAC 2341 TGTTGTAATT CATTAAGCAT TCTGCCGACA TGGAAGCCAT CACAAACGGC ATGATGAACC 2401 TGAATCGCCA GCGGCATCAG CACCTTGTCG CCTTGCGTAT AATATTTGCC CATGGGATCG 2461 ATCGATCGAT Cggatcctct agagtcgacc tgcaggcatg caagcttgcg gccgcgtcgt 2521 gactgggaaa accctggcga ctagtcttgg actcctgttg atagatccag taatgacctc 2581 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga 2641 gaatccaggg gtccccaata attacgattt aaattggcga aaatgagacg ttgatcggca 2701 cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg tattttttga 2761 gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac 2821 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc 2881 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa 2941 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc 3001 tcatccggaa tttcgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca 3061 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata 3121 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga 3181 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc 3241 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc 3301 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat 3361 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca 3421 acagtactgc gatgagtggc agggcggggc gtaatttgac ttttgtcctt ttccgctgca 3481 taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga 3541 tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag 3601 ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct 3661 tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc gtaggccggc 3721 cgcgatgcag gtggctgctg aacccccagc cggaactgac cccacaaggc cctagcgttt 3781 gcaatgcacc aggtcatcat tgacccaggc gtgttccacc aggccgctgc ctcgcaactc 3841 ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg cgggtggaat ccgatccgca 3901 catgaggcgg aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc 3961 gaacatccgt cgggccgtcg gcgacagctt gcggtacttc tcccatatga atttcgtgta 4021 gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc aggacctggc aacgggacgt 4081 tttcttgcca cggtccagga cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc 4141 aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc 4201 cttcgtgtaa taccggccat tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt 4261 gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac tccaacacct gctgccacac 4321 cagttcgtca tcgtcggccc gcagctcgac gccggtgtag gtgatcttca cgtccttgtt 4381 gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt 4441 gaacagggca gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc 4501 aatatcgaac aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc 4561 ggcctgcttg gcttcgctga cctgttttgc caggtcctcg ccggcggttt ttcgcttctt 4621 ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc gccaaacctg ccgcctcctg 4681 ttcgagacga cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag ggggagccag 4741 ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg actatcgagc cgacggactg 4801 gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg atggtttcgg catcctcggc 4861 ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg tccgattcat 4921 tcaccctcct tgcgggattg ccccggaatt aattccccgg atcgatccgt cgatcttgat 4981 cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc 5041 ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt ccataaaacc 5101 gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct ctttgcgctt 5161 gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc 5221 tgcggactgg ctttctacgt ggctgccatt tttggggtga ggccgttcgc ggccgagggg 5281 cgcagcccct ggggggatgg gaggcccgcg ttagcgggcc gggagggttc gagaaggggg 5341 ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt taaaaacaag 5401 gtttataaat attggtttaa aagcaggtta aaagacaggt tagcggtggc cgaaaaacgg 5461 gcggaaaccc ttgcaaatgc tggattttct gcctgtggac agcccctcaa atgtcaatag 5521 gtgcgcccct catctgtcag cactctgccc ctcaagtgtc aaggatcgcg cccctcatct 5581 gtcagtagtc gcgcccctca agtgtcaata ccgcagggca cttatcccca ggcttgtcca 5641 catcatctgt gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg aggctggcca 5701 gctccacgtc gccggccgaa atcgagcctg cccctcatct gtcaacgccg cgccgggtga 5761 gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca agttttccgc 5821 gaggtatcca caacgccggc ggccctacat ggctctgctg tagtgagtgg gttgcgctcc 5881 ggcagcggtc ctgatccccc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 5941 tacggcgcgc ccagctgtct agggcggcgg atttgtccta ctcaggagag cgttcaccga 6001 caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg 6061 cct //