LOCUS IBMc414 6066 bp ds-DNA circular 18-NOV-2020 DEFINITION pSEVA321-PBAD(SapI-)-B30-SrpR(1-171)-RSG-M86N-T ACCESSION IBMc414 VERSION IBMc414.1 FEATURES Location/Qualifiers promoter 984..1267 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" promoter 2700..2797 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" CDS complement(78..956) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" CDS 1819..2118 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86N" CDS 2798..3457 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat" primer_bind 5964..5981 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS1 Fwd" misc_feature 57..77 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" CDS 1297..1809 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="SrpR" RBS 1276..1290 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" primer_bind complement(2607..2623) /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS2 Rev" terminator complement(2228..2284) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" CDS complement(2313..2453) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" CDS complement(3775..4923) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="trfA CDS (replication protein)" terminator 2553..2647 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="lambda t0 terminator" terminator 2130..2219 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" ORIGIN 1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt 61 cgcggccgct tctagagtta tgacaacttg acggctacat cattcacttt ttcttcacaa 121 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 181 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 241 aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 301 aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 361 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 421 acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 481 aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 541 ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 601 aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 661 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 721 atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 781 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 841 ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 901 agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 961 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 1021 cactgcgtct tttactggcA cttctcgcta accaaaccgg taaccccgct tattaaaagc 1081 attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 1141 cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 1201 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 1261 tctccattac tagagattaa agaggagaaa tactagATGG CACGTAAAAC CGCAGCAGAA 1321 GCAGAAGAAA CCCGTCAGCG TATTATTGAT GCAGCACTGG AAGTTTTTGT TGCACAGGGT 1381 GTTAGTGATG CAACCCTGGA TCAGATTGCA CGTAAAGCCG GTGTTACCCG TGGTGCAGTT 1441 TATTGGCATT TTAATGGTAA ACTGGAAGTT CTGCAAGCAG TTCTGGCAAG CCGTCAGCAT 1501 CCGCTGGAAC TGGATTTTAC ACCGGATCTG GGTATTGAAC GTAGCTGGGA AGCAGTTGTT 1561 GTTGCAATGC TGGATGCAGT TCATAGTCCG CAGAGCAAAC AGTTTAGCGA AATTCTGATT 1621 TATCAGGGTC TGGATGAAAG CGGTCTGATT CATAATCGTA TGGTTCAGGC AAGCGATCGT 1681 TTTCTGCAAT ATATTCATCA GGTTCTGCGT CATGCAGTTA CCCAGGGTGA ACTGCCGATT 1741 AATCTGGATC TGCAAACCAG CATTGGTGTT TTTAAAGGTC TGATTACCGG TCTGCTGTAT 1801 GAAGGTCTGC GTAGTGGAtg catctcggga gatagtttga tcagcttggc gagcacaggg 1861 aaaagagttc ctattaagga tttgttaggc gaaaaagatt ttgaaatatg ggcaattaat 1921 gaacagacga tgaagctgga atcagctaaa gttagtcgtg tattttgtac cggcaaaaag 1981 ctagtctata ctctaaaaac tcgactaggt agaactatca aggcaacagc aaatcataga 2041 tttttaacta ttgatggttg gaaaagatta gatgagctat ctttaaaaga gcatattgct 2101 ctaccccgta aactagaaTA ATACTAGAGt tgagaagaga aaagaaaacc gccgatcctg 2161 tccaccgcat tactgcaagg tagtggacaa gaccggcggt cttaagtttt ttggctgaaT 2221 ACTAGAGcct ttggtcgAAA AAAAAAGCCC GCACTGTCAG GTGCGGGCTT TTTTctgtgt 2281 ttccTACTAG AGCAAGCGAG CTCGATATCA AATTACGCCC CGCCCTGCCA CTCATCGCAG 2341 TACTGTTGTA ATTCATTAAG CATTCTGCCG ACATGGAAGC CATCACAAAC GGCATGATGA 2401 ACCTGAATCG CCAGCGGCAT CAGCACCTTG TCGCCTTGCG TATAATATTT GCCCATGGGA 2461 TCGATCGATC GATCggatcc tctagagtcg acctgcaggc atgcaagctt gcggccgcgt 2521 cgtgactggg aaaaccctgg cgactagtct tggactcctg ttgatagatc cagtaatgac 2581 ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg 2641 tgagaatcca ggggtcccca ataattacga tttaaattgg cgaaaatgag acgttgatcg 2701 gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt 2761 tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata 2821 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt 2881 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt 2941 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa 3001 tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt 3061 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga 3121 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg 3181 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa 3241 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc 3301 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc 3361 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt 3421 acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt gacttttgtc cttttccgct 3481 gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc ctttttcgca 3541 cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat ccaacggcgt 3601 cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt cttcactgtc 3661 ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg gccgtaggcc 3721 ggccgcgatg caggtggctg ctgaaccccc agccggaact gaccccacaa ggccctagcg 3781 tttgcaatgc accaggtcat cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa 3841 ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc 3901 gcacatgagg cggaaggttt ccagcttgag cgggtacggc tcccggtgcg agctgaaata 3961 gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac ttctcccata tgaatttcgt 4021 gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct ggcaacggga 4081 cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg attccaggtg 4141 cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc 4201 ggccttcgtg taataccggc cattgatcga ccagcccagg tcctggcaaa gctcgtagaa 4261 cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg tactccaaca cctgctgcca 4321 caccagttcg tcatcgtcgg cccgcagctc gacgccggtg taggtgatct tcacgtcctt 4381 gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt 4441 ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg 4501 cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa 4561 cgcggcctgc ttggcttcgc tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt 4621 cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc 4681 ctgttcgaga cgacgcgaac gctccacggc ggccgatggc gcgggcaggg cagggggagc 4741 cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc tggactatcg agccgacgga 4801 ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt cggcatcctc 4861 ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt 4921 cattcaccct ccttgcggga ttgccccgga attaattccc cggatcgatc cgtcgatctt 4981 gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc 5041 ttcccaacct taccagaggg cgccccagct ggcaattccg gttcgcttgc tgtccataaa 5101 accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt tctctttgcg 5161 cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggg tcagcaccgt 5221 ttctgcggac tggctttcta cgtggctgcc atttttgggg tgaggccgtt cgcggccgag 5281 gggcgcagcc cctgggggga tgggaggccc gcgttagcgg gccgggaggg ttcgagaagg 5341 gggggcaccc cccttcggcg tgcgcggtca cgcgcacagg gcgcagccct ggttaaaaac 5401 aaggtttata aatattggtt taaaagcagg ttaaaagaca ggttagcggt ggccgaaaaa 5461 cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg gacagcccct caaatgtcaa 5521 taggtgcgcc cctcatctgt cagcactctg cccctcaagt gtcaaggatc gcgcccctca 5581 tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc ccaggcttgt 5641 ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt gcgaggctgg 5701 ccagctccac gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg ccgcgccggg 5761 tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg ccaagttttc 5821 cgcgaggtat ccacaacgcc ggcggcccta catggctctg ctgtagtgag tgggttgcgc 5881 tccggcagcg gtcctgatcc cccgcagaaa aaaaggatct caagaagatc ctttgatctt 5941 ttctacggcg cgcccagctg tctagggcgg cggatttgtc ctactcagga gagcgttcac 6001 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg 6061 atgcct //