LOCUS IBMc415 6078 bp ds-DNA circular 18-NOV-2020 DEFINITION pSEVA321-PBAD(SapI-)-B30-SrpR(1-175)-QHG-M86N-T ACCESSION IBMc415 VERSION IBMc415.1 FEATURES Location/Qualifiers promoter 2712..2809 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" CDS 1297..1821 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="SrpR" CDS 2810..3469 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat" RBS 1276..1290 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" primer_bind 5976..5993 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS1 Fwd" misc_feature 57..77 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" primer_bind complement(2619..2635) /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS2 Rev" promoter 984..1267 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" terminator complement(2240..2296) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" CDS complement(3787..4935) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="trfA CDS (replication protein)" CDS 1831..2130 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86N" terminator 2565..2659 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="lambda t0 terminator" CDS complement(78..956) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" terminator 2142..2231 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" CDS complement(2325..2465) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" ORIGIN 1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt 61 cgcggccgct tctagagtta tgacaacttg acggctacat cattcacttt ttcttcacaa 121 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 181 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 241 aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 301 aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 361 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 421 acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 481 aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 541 ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 601 aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 661 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 721 atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 781 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 841 ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 901 agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 961 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 1021 cactgcgtct tttactggcA cttctcgcta accaaaccgg taaccccgct tattaaaagc 1081 attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 1141 cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 1201 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 1261 tctccattac tagagattaa agaggagaaa tactagATGG CACGTAAAAC CGCAGCAGAA 1321 GCAGAAGAAA CCCGTCAGCG TATTATTGAT GCAGCACTGG AAGTTTTTGT TGCACAGGGT 1381 GTTAGTGATG CAACCCTGGA TCAGATTGCA CGTAAAGCCG GTGTTACCCG TGGTGCAGTT 1441 TATTGGCATT TTAATGGTAA ACTGGAAGTT CTGCAAGCAG TTCTGGCAAG CCGTCAGCAT 1501 CCGCTGGAAC TGGATTTTAC ACCGGATCTG GGTATTGAAC GTAGCTGGGA AGCAGTTGTT 1561 GTTGCAATGC TGGATGCAGT TCATAGTCCG CAGAGCAAAC AGTTTAGCGA AATTCTGATT 1621 TATCAGGGTC TGGATGAAAG CGGTCTGATT CATAATCGTA TGGTTCAGGC AAGCGATCGT 1681 TTTCTGCAAT ATATTCATCA GGTTCTGCGT CATGCAGTTA CCCAGGGTGA ACTGCCGATT 1741 AATCTGGATC TGCAAACCAG CATTGGTGTT TTTAAAGGTC TGATTACCGG TCTGCTGTAT 1801 GAAGGTCTGC GTAGCAAAGA TCAGCATGGA tgcatctcgg gagatagttt gatcagcttg 1861 gcgagcacag ggaaaagagt tcctattaag gatttgttag gcgaaaaaga ttttgaaata 1921 tgggcaatta atgaacagac gatgaagctg gaatcagcta aagttagtcg tgtattttgt 1981 accggcaaaa agctagtcta tactctaaaa actcgactag gtagaactat caaggcaaca 2041 gcaaatcata gatttttaac tattgatggt tggaaaagat tagatgagct atctttaaaa 2101 gagcatattg ctctaccccg taaactagaa TAATACTAGA Gttgagaaga gaaaagaaaa 2161 ccgccgatcc tgtccaccgc attactgcaa ggtagtggac aagaccggcg gtcttaagtt 2221 ttttggctga aTACTAGAGc ctttggtcgA AAAAAAAAGC CCGCACTGTC AGGTGCGGGC 2281 TTTTTTctgt gtttccTACT AGAGCAAGCG AGCTCGATAT CAAATTACGC CCCGCCCTGC 2341 CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA GCCATCACAA 2401 ACGGCATGAT GAACCTGAAT CGCCAGCGGC ATCAGCACCT TGTCGCCTTG CGTATAATAT 2461 TTGCCCATGG GATCGATCGA TCGATCggat cctctagagt cgacctgcag gcatgcaagc 2521 ttgcggccgc gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga 2581 tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc 2641 gttttttatt ggtgagaatc caggggtccc caataattac gatttaaatt ggcgaaaatg 2701 agacgttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc 2761 gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa 2821 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc 2881 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt 2941 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc 3001 ccgcctgatg aatgctcatc cggaatttcg tatggcaatg aaagacggtg agctggtgat 3061 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc 3121 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt 3181 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt 3241 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga 3301 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct 3361 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat 3421 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttgacttttg 3481 tccttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca 3541 tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt 3601 atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc 3661 ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc 3721 tggccgtagg ccggccgcga tgcaggtggc tgctgaaccc ccagccggaa ctgaccccac 3781 aaggccctag cgtttgcaat gcaccaggtc atcattgacc caggcgtgtt ccaccaggcc 3841 gctgcctcgc aactcttcgc aggcttcgcc gacctgctcg cgccacttct tcacgcgggt 3901 ggaatccgat ccgcacatga ggcggaaggt ttccagcttg agcgggtacg gctcccggtg 3961 cgagctgaaa tagtcgaaca tccgtcgggc cgtcggcgac agcttgcggt acttctccca 4021 tatgaatttc gtgtagtggt cgccagcaaa cagcacgacg atttcctcgt cgatcaggac 4081 ctggcaacgg gacgttttct tgccacggtc caggacgcgg aagcggtgca gcagcgacac 4141 cgattccagg tgcccaacgc ggtcggacgt gaagcccatc gccgtcgcct gtaggcgcga 4201 caggcattcc tcggccttcg tgtaataccg gccattgatc gaccagccca ggtcctggca 4261 aagctcgtag aacgtgaagg tgatcggctc gccgataggg gtgcgcttcg cgtactccaa 4321 cacctgctgc cacaccagtt cgtcatcgtc ggcccgcagc tcgacgccgg tgtaggtgat 4381 cttcacgtcc ttgttgacgt ggaaaatgac cttgttttgc agcgcctcgc gcgggatttt 4441 cttgttgcgc gtggtgaaca gggcagagcg ggccgtgtcg tttggcatcg ctcgcatcgt 4501 gtccggccac ggcgcaatat cgaacaagga aagctgcatt tccttgatct gctgcttcgt 4561 gtgtttcagc aacgcggcct gcttggcttc gctgacctgt tttgccaggt cctcgccggc 4621 ggtttttcgc ttcttggtcg tcatagttcc tcgcgtgtcg atggtcatcg acttcgccaa 4681 acctgccgcc tcctgttcga gacgacgcga acgctccacg gcggccgatg gcgcgggcag 4741 ggcaggggga gccagttgca cgctgtcgcg ctcgatcttg gccgtagctt gctggactat 4801 cgagccgacg gactggaagg tttcgcgggg cgcacgcatg acggtgcggc ttgcgatggt 4861 ttcggcatcc tcggcggaaa accccgcgtc gatcagttct tgcctgtatg ccttccggtc 4921 aaacgtccga ttcattcacc ctccttgcgg gattgccccg gaattaattc cccggatcga 4981 tccgtcgatc ttgatcccct gcgccatcag atccttggcg gcaagaaagc catccagttt 5041 actttgcagg gcttcccaac cttaccagag ggcgccccag ctggcaattc cggttcgctt 5101 gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc 5161 tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg 5221 ggtcagcacc gtttctgcgg actggctttc tacgtggctg ccatttttgg ggtgaggccg 5281 ttcgcggccg aggggcgcag cccctggggg gatgggaggc ccgcgttagc gggccgggag 5341 ggttcgagaa gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc 5401 ctggttaaaa acaaggttta taaatattgg tttaaaagca ggttaaaaga caggttagcg 5461 gtggccgaaa aacgggcgga aacccttgca aatgctggat tttctgcctg tggacagccc 5521 ctcaaatgtc aataggtgcg cccctcatct gtcagcactc tgcccctcaa gtgtcaagga 5581 tcgcgcccct catctgtcag tagtcgcgcc cctcaagtgt caataccgca gggcacttat 5641 ccccaggctt gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat 5701 ttgcgaggct ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa 5761 cgccgcgccg ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag 5821 ggccaagttt tccgcgaggt atccacaacg ccggcggccc tacatggctc tgctgtagtg 5881 agtgggttgc gctccggcag cggtcctgat cccccgcaga aaaaaaggat ctcaagaaga 5941 tcctttgatc ttttctacgg cgcgcccagc tgtctagggc ggcggatttg tcctactcag 6001 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt 6061 tcgttttatt tgatgcct //