LOCUS IBMc429 5467 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(177-213)-Ter ACCESSION IBMc429 VERSION IBMc429.1 FEATURES Location/Qualifiers CDS 3556..3666 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="SrpR" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" CDS complement(1517..2035) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" misc_feature 3286..3360 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="RiboJ" /featureType="insulator" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" RBS 2253..2280 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="phl2" primer_bind complement(3846..3865) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" promoter 2199..2252 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="PlacIQ" CDS 3391..3552 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86C" terminator 3148..3200 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="L3S3P21" promoter 3220..3285 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="PPhlF" terminator 2893..3147 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="IOT" terminator 2134..2194 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="L3S2P21" terminator 3678..3730 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" RBS 3369..3381 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="BBa_B0032" misc_feature 3731..3751 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" misc_feature 1494..1494 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" promoter complement(2036..2133) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" rep_origin 4312..5136 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" CDS 2281..2883 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="PhlF" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT 1561 TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA AACGAAAAAC ATATTCTCAA 1621 TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA 1681 TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG 1741 TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT 1801 CTTTCATTGC CATACGAAAT TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA 1861 AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA 1921 GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT 1981 TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATTTTAG 2041 CTTCCTTAGC TCCTGAAAAT CTCGATAACT CAAAAAATAC GCCCGGTAGT GATCTTATTT 2101 CATTATGGTG AAAGTTGGAA CCTCTTACGT GCCctcggta ccaaattcca gaaaagaggc 2161 ctcccgaaag gggggccttt tttcgttttg gtccaatggc ggcgcgccat cgaatggtgc 2221 aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcat gggggtgaat 2281 atggcacgta ccccgagccg tagcagcatt ggtagcctgc gtagtccgca tacccataaa 2341 gcaattctga ccagcaccat tgaaatcctg aaagaatgtg gttatagcgg tctgagcatt 2401 gaaagcgtgg cacgtcgcgc cggtgcaggc aaaccgacca tttatcgttg gtggaccaac 2461 aaagcagcac tgattgccga agtgtatgaa aatgaaatcg aacaggtacg taaatttccg 2521 gatttgggta gctttaaagc cgatctggat tttctgctgc ataatctgtg gaaagtttgg 2581 cgtgaaacca tttgtggtga agcatttcgt tgtgttattg cagaagcaca gttggaccct 2641 gtaaccctga cccaactgaa agatcagttt atggaacgtc gtcgtgagat accgaaaaaa 2701 ctggttgaag atgccattag caatggtgaa ctgccgaaag atatcaatcg tgaactgctg 2761 ctggatatga tttttggttt ttgttggtat cgcctgctga ccgaacagtt gaccgttgaa 2821 caggatattg aagaatttac cttcctgctg attaatggtg tttgtccggg tacacagtgt 2881 tgataaggat cctaattggt aacgaatcag acaattgacg gctcgaggga gtagcatagg 2941 gtttgcagaa tccctgcttc gtccatttga caggcacatt atgcatcgat gataagctgt 3001 caaacatgag cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg 3061 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt 3121 cgggctcatg agcaaatatt ttatctgcca attattgaag gcctccctaa cggggggcct 3181 ttttttgttt ctggtctccc gcttaacgat cgttggctgc gacgtacggt ggaatctgat 3241 tcgttaccaa ttgacatgat acgaaacgta ccgtatcgtt aaggtagctg tcaccggatg 3301 tgctttccgg tctgatgagt ccgtgaggac gaaacagcct ctacaaataa ttttgtttaa 3361 tactagagtc acacaggaaa gtactagATG agctcctctt tacaattggc accagaaata 3421 gaaaagttgc ctcagagtga tatttactgg gaccccatcg tttctattac ggagactgga 3481 gtcgaagagg tttttgattt gactgtgcca ggactacgta actttgtcgc caatgacatc 3541 attgtacata acTCACAGGC ACAGATTATC AAAGTTGCAC TGGGTAGCTT TTGGGCACTG 3601 CTGCGTGAAC CGCCTCGTTT TCTGCTGTGT GAAGAAGCAC AGATTAAACA GGTGAAATCC 3661 TTCGAATAAt actagagaac gcatgagAAA GCCCCCGGAA GATCACCTTC CGGGGGCTTT 3721 tttattgcgc tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta 3781 gcagaaagtc aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca 3841 ttggggctca ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaaggatg 3901 atttctgcta gagatggaat agactggatg gaggcggata aagttgcagg accacttctg 3961 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtgga 4021 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 4081 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 4141 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt 4201 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 4261 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cttaataaga 4321 tgatcttctt gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc 4381 gccttgcagg gcggtttttc gaaggttctc tgagctacca actctttgaa ccgaggtaac 4441 tggcttggag gagcgcagtc accaaaactt gtcctttcag tttagcctta accggcgcat 4501 gacttcaaga ctaactcctc taaatcaatt accagtggct gctgccagtg gtgcttttgc 4561 atgtctttcc gggttggact caagacgata gttaccggat aaggcgcagc ggtcggactg 4621 aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt 4681 caggcgtgga atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg 4741 caggaacagg agagcgcacg agggagccgc caggggaaac gcctggtatc tttatagtcc 4801 tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg 4861 gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag tatcttcctg 4921 gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg cagtcgaacg 4981 accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca catattctgc 5041 tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag cacttcactg acaccctcat 5101 cagtgccaac atagtaagcc agtatacact ccgctagcgc tgaggtctgc ctcgtgaaga 5161 aggtgttgct gactcatacc aggcctgaat cgccccatca tccagccaga aagtgaggga 5221 gccacggttg atgagagctt tgttgtaggt ggaccagttg gtgattttga acttttgctt 5281 tgccacggaa cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca actcagcaaa 5341 agttcgattt attcaacaaa gccacgttgt gtctcaaaat ctctgatgtt acattgcaca 5401 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag 5461 gggtgtt //