LOCUS IBMc451 4943 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PPhlF-RiboJ-BCD1-SYNZIP18-5aa-mCherry*(186-236)-H6-Te r ACCESSION IBMc451 VERSION IBMc451.1 FEATURES Location/Qualifiers RBS 2816..2824 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="RBS for CDS2" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" RBS 2765..2772 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="RBS for CDS 1" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" misc_feature 2668..2742 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="RiboJ" /featureType="insulator" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" promoter 2602..2667 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="PPhlF" primer_bind complement(3322..3341) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" misc_feature 2746..2830 /ApEinfo_fwdcolor="#008000" /ApEinfo_revcolor="#008000" /label="BCD1" terminator 2530..2582 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="L3S3P21" rep_origin 3788..4612 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" terminator 2275..2529 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="IOT" misc_feature 3207..3227 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" CDS 1663..2265 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="PhlF" terminator 3154..3206 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" RBS 1635..1662 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="phl2" CDS 3125..3142 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="H6" promoter 1581..1634 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="PlacIQ" CDS 2972..3124 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(186-236)" terminator 1516..1576 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="L3S2P21" CDS 2831..2956 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="SYNZIP18" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagctcgg taccaaattc cagaaaagag gcctcccgaa aggggggcct 1561 tttttcgttt tggtccaatg gcggcgcgcc atcgaatggt gcaaaacctt tcgcggtatg 1621 gcatgatagc gcccggaaga gagtcaattc atgggggtga atatggcacg taccccgagc 1681 cgtagcagca ttggtagcct gcgtagtccg catacccata aagcaattct gaccagcacc 1741 attgaaatcc tgaaagaatg tggttatagc ggtctgagca ttgaaagcgt ggcacgtcgc 1801 gccggtgcag gcaaaccgac catttatcgt tggtggacca acaaagcagc actgattgcc 1861 gaagtgtatg aaaatgaaat cgaacaggta cgtaaatttc cggatttggg tagctttaaa 1921 gccgatctgg attttctgct gcataatctg tggaaagttt ggcgtgaaac catttgtggt 1981 gaagcatttc gttgtgttat tgcagaagca cagttggacc ctgtaaccct gacccaactg 2041 aaagatcagt ttatggaacg tcgtcgtgag ataccgaaaa aactggttga agatgccatt 2101 agcaatggtg aactgccgaa agatatcaat cgtgaactgc tgctggatat gatttttggt 2161 ttttgttggt atcgcctgct gaccgaacag ttgaccgttg aacaggatat tgaagaattt 2221 accttcctgc tgattaatgg tgtttgtccg ggtacacagt gttgataagg atcctaattg 2281 gtaacgaatc agacaattga cggctcgagg gagtagcata gggtttgcag aatccctgct 2341 tcgtccattt gacaggcaca ttatgcatcg atgataagct gtcaaacatg agcagatcct 2401 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta 2461 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcaaata 2521 ttttatctgc caattattga aggcctccct aacggggggc ctttttttgt ttctggtctc 2581 ccgcttaacg atcgttggct gcgacgtacg gtggaatctg attcgttacc aattgacatg 2641 atacgaaacg taccgtatcg ttaaggtagc tgtcaccgga tgtgctttcc ggtctgatga 2701 gtccgtgagg acgaaacagc ctctacaaat aattttgttt aataaGGGCC CAAGTTCACT 2761 TAAAAAGGAG ATCAACAATG AAAGCAATTT TCGTACTGAA ACATCTTAAT CATGCACAGG 2821 AGACTTTCTA atgagcatcg cggcgaccct ggagaacgat ctggcgcgtc tggaaaacga 2881 aaacgctcgt ctcgaaaaag acatcgcgaa cctggaacgt gacctggcga aactggagcg 2941 tgaagaagcg tacttcggag gttcaggtgg ttacaaggcc aagaagcccg tgcagctgcc 3001 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat 3061 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta 3121 caagcatcat caccatcacc actaatacta gagaacgcat gagAAAGCCC CCGGAAGATC 3181 ACCTTCCGGG GGCTTTttta ttgcgctact agtagcggcc gctgcaggag tcactaaggg 3241 ttagttagtt agattagcag aaagtcaaaa gcctccgacc ggaggctttt gactaaaact 3301 tcccttgggg ttatcattgg ggctcactca aaggcggtaa tcagataaaa aaaatcctta 3361 gctttcgcta aggatgattt ctgctagaga tggaatagac tggatggagg cggataaagt 3421 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 3481 agccggtgag cgtggatctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 3541 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 3601 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 3661 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 3721 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 3781 agacccctta ataagatgat cttcttgaga tcgttttggt ctgcgcgtaa tctcttgctc 3841 tgaaaacgaa aaaaccgcct tgcagggcgg tttttcgaag gttctctgag ctaccaactc 3901 tttgaaccga ggtaactggc ttggaggagc gcagtcacca aaacttgtcc tttcagttta 3961 gccttaaccg gcgcatgact tcaagactaa ctcctctaaa tcaattacca gtggctgctg 4021 ccagtggtgc ttttgcatgt ctttccgggt tggactcaag acgatagtta ccggataagg 4081 cgcagcggtc ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct 4141 acccggaact gagtgtcagg cgtggaatga gacaaacgcg gccataacag cggaatgaca 4201 ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg agccgccagg ggaaacgcct 4261 ggtatcttta tagtcctgtc gggtttcgcc accactgatt tgagcgtcag atttcgtgat 4321 gcttgtcagg ggggcggagc ctatggaaaa acggctttgc cgcggccctc tcacttccct 4381 gttaagtatc ttcctggcat cttccaggaa atctccgccc cgttcgtaag ccatttccgc 4441 tcgccgcagt cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg aatatatcct 4501 gtatcacata ttctgctgac gcaccggtgc agcctttttt ctcctgccac atgaagcact 4561 tcactgacac cctcatcagt gccaacatag taagccagta tacactccgc tagcgctgag 4621 gtctgcctcg tgaagaaggt gttgctgact cataccaggc ctgaatcgcc ccatcatcca 4681 gccagaaagt gagggagcca cggttgatga gagctttgtt gtaggtggac cagttggtga 4741 ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc gggaagatgc gtgatctgat 4801 ccttcaactc agcaaaagtt cgatttattc aacaaagcca cgttgtgtct caaaatctct 4861 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca 4921 taaacagtaa tacaaggggt gtt //