LOCUS IBMc459 5391 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B32-bla-Ter ACCESSION IBMc459 VERSION IBMc459.1 FEATURES Location/Qualifiers CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" primer_bind complement(3770..3789) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" RBS 2714..2726 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="BBa_B0032" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" terminator 3602..3654 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" misc_feature 3655..3675 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" rep_origin 4236..5060 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" CDS 2733..3590 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="TEM-1 bla" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcAct tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagtcacaca ggaaagtact agatgagtat tcaacatttc cgtgtcgccc 2761 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 2821 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg tTACatcgaa ctggatctca 2881 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 2941 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 3001 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 3061 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 3121 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 3181 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 3241 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 3301 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 3361 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 3421 ctgataaatc tggagccggt gagcgtGGAt ctcgcggtat cattgcagca ctggggccag 3481 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 3541 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg TAAtactaga 3601 gaacgcatga gAAAGCCCCC GGAAGATCAC CTTCCGGGGG CTTTtttatt gcgctactag 3661 tagcggccgc tgcaggagtc actaagggtt agttagttag attagcagaa agtcaaaagc 3721 ctccgaccgg aggcttttga ctaaaacttc ccttggggtt atcattgggg ctcactcaaa 3781 ggcggtaatc agataaaaaa aatccttagc tttcgctaag gatgatttct gctagagatg 3841 gaatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 3901 ctggctggtt tattgctgat aaatctggag ccggtgagcg tggatctcgc ggtatcattg 3961 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 4021 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 4081 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 4141 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 4201 aacgtgagtt ttcgttccac tgagcgtcag accccttaat aagatgatct tcttgagatc 4261 gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg cagggcggtt 4321 tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt ggaggagcgc 4381 agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc aagactaact 4441 cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct ttccgggttg 4501 gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg gggttcgtgc 4561 atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg tggaatgaga 4621 caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg 4681 cacgagggag ccgccagggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 4741 cactgatttg agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac 4801 ggctttgccg cggccctctc acttccctgt taagtatctt cctggcatct tccaggaaat 4861 ctccgccccg ttcgtaagcc atttccgctc gccgcagtcg aacgaccgag cgtagcgagt 4921 cagtgagcga ggaagcggaa tatatcctgt atcacatatt ctgctgacgc accggtgcag 4981 ccttttttct cctgccacat gaagcacttc actgacaccc tcatcagtgc caacatagta 5041 agccagtata cactccgcta gcgctgaggt ctgcctcgtg aagaaggtgt tgctgactca 5101 taccaggcct gaatcgcccc atcatccagc cagaaagtga gggagccacg gttgatgaga 5161 gctttgttgt aggtggacca gttggtgatt ttgaactttt gctttgccac ggaacggtct 5221 gcgttgtcgg gaagatgcgt gatctgatcc ttcaactcag caaaagttcg atttattcaa 5281 caaagccacg ttgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca 5341 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt t //