LOCUS IBMc461 4654 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B32-bla(1-28)-BsaI-BsaI-bla(285-286)-Ter ACCESSION IBMc461 VERSION IBMc461.1 FEATURES Location/Qualifiers promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" terminator 2865..2917 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" misc_feature 2918..2938 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" rep_origin 3499..4323 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" CDS 2733..2817 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="bla" CDS 2848..2853 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="bla" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" RBS 2714..2726 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="BBa_B0032" primer_bind complement(3033..3052) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" misc_feature 2825..2840 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="spacing sequence" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcAct tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagtcacaca ggaaagtact agatgagtat tcaacatttc cgtgtcgccc 2761 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggTGA 2821 GACCacggcc acatcgtgga GGTCTCAcat tggTAAtact agagaacgca tgagAAAGCC 2881 CCCGGAAGAT CACCTTCCGG GGGCTTTttt attgcgctac tagtagcggc cgctgcagga 2941 gtcactaagg gttagttagt tagattagca gaaagtcaaa agcctccgac cggaggcttt 3001 tgactaaaac ttcccttggg gttatcattg gggctcactc aaaggcggta atcagataaa 3061 aaaaatcctt agctttcgct aaggatgatt tctgctagag atggaataga ctggatggag 3121 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 3181 gataaatctg gagccggtga gcgtggatct cgcggtatca ttgcagcact ggggccagat 3241 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa 3301 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac 3361 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 3421 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 3481 cactgagcgt cagacccctt aataagatga tcttcttgag atcgttttgg tctgcgcgta 3541 atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga 3601 gctaccaact ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc 3661 ctttcagttt agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc 3721 agtggctgct gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt 3781 accggataag gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga 3841 gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca 3901 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag 3961 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc caccactgat ttgagcgtca 4021 gatttcgtga tgcttgtcag gggggcggag cctatggaaa aacggctttg ccgcggccct 4081 ctcacttccc tgttaagtat cttcctggca tcttccagga aatctccgcc ccgttcgtaa 4141 gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg 4201 gaatatatcc tgtatcacat attctgctga cgcaccggtg cagccttttt tctcctgcca 4261 catgaagcac ttcactgaca ccctcatcag tgccaacata gtaagccagt atacactccg 4321 ctagcgctga ggtctgcctc gtgaagaagg tgttgctgac tcataccagg cctgaatcgc 4381 cccatcatcc agccagaaag tgagggagcc acggttgatg agagctttgt tgtaggtgga 4441 ccagttggtg attttgaact tttgctttgc cacggaacgg tctgcgttgt cgggaagatg 4501 cgtgatctga tccttcaact cagcaaaagt tcgatttatt caacaaagcc acgttgtgtc 4561 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact 4621 gtctgcttac ataaacagta atacaagggg tgtt //