LOCUS IBMc477 3864 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB1A3-PBAD(SapI-)-B30-mCherry*(1-121)-5aa-SYNZIP17-Ter ACCESSION IBMc477 VERSION IBMc477.1 FEATURES Location/Qualifiers RBS 2316..2330 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" primer_bind 982..1001 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" CDS complement(1..861) /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="ampR" CDS 2715..2840 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="SYNZIP17" terminator 2865..2921 /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="L3S2P55" promoter 2024..2307 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" rep_origin complement(3091..3705) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="rep (pMB1)" CDS 2337..2699 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(1-121)" CDS complement(1118..1996) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" primer_bind complement(2985..3004) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" ORIGIN 1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 121 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 301 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 721 gttcgatata acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 841 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 961 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 1021 taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt 1081 tcgctaagga tgatttctgg aattcgagct cggtacctta tgacaacttg acggctacat 1141 cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt 1201 taaatacccg cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga 1261 taggcatccg ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc 1321 agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca 1381 agcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga 1441 tgtactgaca agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg 1501 cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc 1561 gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt 1621 gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat tgacggccag ttaagccatt 1681 catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg 1741 gatgacgacc gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc 1801 aaacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa 1861 tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa 1921 tcggcgttaa acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat 1981 tttgcgcttc agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat 2041 attgcatcag acattgccgt cactgcgtct tttactggcA cttctcgcta accaaaccgg 2101 taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc 2161 gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca 2221 cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt 2281 tatcgcaact ctctactgtt tctccattac tagagattaa agaggagaaa tactagatgg 2341 tgagcaaggg cgaagaaGAT aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc 2401 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc 2461 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg 2521 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg 2581 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga 2641 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccttg caggacggcg 2701 gaggtggtgg atccaacgaa aaagaagaac tgaaatccaa aaaagcggaa ctgcgcaacc 2761 gtatcgaaca gctgaaacag aaacgtgaac aactgaagca gaaaatcgcg aacctgcgta 2821 aagaaatcga agcttacaaa taaGGGCCCA AGTTCACTTA AAAACTCGGT ACCAAAGACG 2881 AACAATAAGA CGCTGAAAAG CGTCTTTTTT CGTTTTGGTC Cctgcaggct tcctcgctca 2941 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 3001 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 3061 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc 3121 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 3181 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 3241 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 3301 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 3361 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 3421 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 3481 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 3541 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 3601 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 3661 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 3721 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 3781 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 3841 atgagtaaac ttggtctgac agtt //