LOCUS IBMc479 4020 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB1A3-PBAD(SapI-)-B30-mCherry*(1-173)-5aa-SYNZIP17-Ter ACCESSION IBMc479 VERSION IBMc479.1 FEATURES Location/Qualifiers primer_bind 982..1001 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" CDS 2871..2996 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="SYNZIP17" terminator 3021..3077 /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="L3S2P55" rep_origin complement(3247..3861) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="rep (pMB1)" CDS complement(1..861) /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="ampR" CDS 2337..2855 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(1-173)" CDS complement(1118..1996) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" promoter 2024..2307 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" primer_bind complement(3141..3160) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" RBS 2316..2330 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" ORIGIN 1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 121 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 301 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 721 gttcgatata acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 841 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 961 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 1021 taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt 1081 tcgctaagga tgatttctgg aattcgagct cggtacctta tgacaacttg acggctacat 1141 cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt 1201 taaatacccg cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga 1261 taggcatccg ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc 1321 agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca 1381 agcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga 1441 tgtactgaca agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg 1501 cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc 1561 gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt 1621 gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat tgacggccag ttaagccatt 1681 catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg 1741 gatgacgacc gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc 1801 aaacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa 1861 tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa 1921 tcggcgttaa acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat 1981 tttgcgcttc agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat 2041 attgcatcag acattgccgt cactgcgtct tttactggcA cttctcgcta accaaaccgg 2101 taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc 2161 gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca 2221 cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt 2281 tatcgcaact ctctactgtt tctccattac tagagattaa agaggagaaa tactagatgg 2341 tgagcaaggg cgaagaaGAT aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc 2401 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc 2461 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg 2521 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg 2581 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga 2641 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccttg caggacggcg 2701 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc 2761 agaagAAAac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc 2821 tgaagggcga gatcaagcag aggctgaagc tgaagggagg tggtggatcc aacgaaaaag 2881 aagaactgaa atccaaaaaa gcggaactgc gcaaccgtat cgaacagctg aaacagaaac 2941 gtgaacaact gaagcagaaa atcgcgaacc tgcgtaaaga aatcgaagct tacaaataaG 3001 GGCCCAAGTT CACTTAAAAA CTCGGTACCA AAGACGAACA ATAAGACGCT GAAAAGCGTC 3061 TTTTTTCGTT TTGGTCCctg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc 3121 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 3181 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 3241 aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc 3301 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 3361 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 3421 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 3481 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 3541 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 3601 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 3661 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 3721 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 3781 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 3841 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 3901 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 3961 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt //