LOCUS       Positive_2016In         2967 bp    DNA     circular     15-May-2024
ACCESSION   Positive_2016Interlab
VERSION     Positive_2016Interlab.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=BioBrick suffix
     stem_loop       22..93
                     /label=E. coli his operon terminator
     stem_loop       30..64
                     /label=Stem loop
     primer_bind     157..176
                     /label=Verification reverse (VR) primer binding site
     misc_feature    261..875
                     /label=rep (pMB1)
     misc_feature    276..276
                     /label=ORI
     stem_loop       1042..1147
                     /label=T0 terminator
     CDS             1160..1819
                     /label=Chloramphenicol resistance
     primer_bind     1933..1952
                     /label=Verification forward (VF2) primer binding site
     stem_loop       2009..2012
                     /label=end of terminator
     stem_loop       2022..2041
                     /label=Stem loop
     misc_feature    2051..2072
                     /label=BioBrick prefix
     misc_feature    1..2070
                     /OBI_0001617=29928012
                     /label=pSB1C3
     promoter        2071..2105
                     /OBI_0001617=29928012
                     /label=BBa_J23151
     RBS             2106..2118
                     /label=RBS-3\Weak
     RBS             2112..2115
                     /label=RBS
     misc_feature    2106..2118
                     /label=BBa_B0032
     RBS             2106..2118
                     /OBI_0001617=29928012
                     /label=BBa_B0032
     CDS             2119..2838
                     /label=GFP protein
     CDS             2119..2838
                     /OBI_0001617=29928012
                     /label=GFP
     stem_loop       2850..2893
                     /label=stem_loop
     misc_feature    2839..2918
                     /label=BBa_B0010
     terminator      2839..2918
                     /label=BBa_B0010
     stem_loop       2934..2953
                     /label=T7 TE
     misc_feature    2960..2960
                     /label=stop
     polyA_site      2954..2967
                     /label=polya
     misc_feature    2927..2967
                     /label=BBa_B0012
     terminator      2927..2967
                     /label=BBa_B0012
     terminator      2839..2967
                     /OBI_0001617=29928012
                     /label=BBa_B0015
ORIGIN
        1 tactagtagc ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt
       61 ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct
      121 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      181 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      241 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga
      301 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      361 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      421 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      481 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      541 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      601 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      661 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
      721 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      781 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      841 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
      901 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
      961 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1021 ttggtctgac agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     1081 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     1141 aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt
     1201 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     1261 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     1321 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     1381 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     1441 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     1501 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     1561 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca
     1621 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     1681 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     1741 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     1801 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     1861 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga
     1921 tcaactcgag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     1981 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
     2041 gatttctgga attcgcggcc gcttctagag ttgatggcta gctcagtcct aggtacaatg
     2101 ctagctcaca caggaaagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
     2161 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
     2221 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
     2281 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
     2341 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
     2401 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
     2461 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
     2521 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
     2581 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
     2641 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
     2701 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
     2761 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
     2821 gaactataca aataataacc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     2881 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     2941 cttcgggtgg gcctttctgc gtttata
//