TOPO

BBa_B0043 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_B0043
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Reshma Shetty
Date created: 2006-02-04 12:00:00
Date modified: 2015-08-31 04:07:20

Topoisomerase I mediated cloning site (forward)



Types
DnaRegion

Roles
DNA

sequence_feature

Sequences BBa_B0043_sequence (Version 1)

Description

Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning.

Sequence Annotation Location Component / Role(s)
Topoisomerase I recognition site
Nt.BstNB I recognition site
Arbitrary sequence (could be any bases)
Generated T overhang
1,5
9,13
6,8
5,5
feature/misc sequence_feature
sequence_feature feature/misc
sequence_feature feature/misc
feature/misc sequence_feature
igem#experience
Fails
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_B0043/1