LOCUS       BBa_B3201               2867 bp    DNA     linear       10-Jun-2024
DEFINITION  I13516 B1201
ACCESSION   BBa_B3201
VERSION     BBa_B3201.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..1911
                     /label=mrfp1
     misc_feature    1918..1942
                     /label=Help:Barcodes
     CDS             1237..1917
                     /label=BBa_E1010
     misc_feature    1..1942
                     /label=BBa_I13516
     stem_loop       1960..1975
                     /label=stem loop
     polyA_site      1976..1984
                     /label=Poly A tail
     polyA_site      1951..1959
                     /label=PolyA tail
     misc_feature    1951..1984
                     /label=B1001
     terminator      1951..1984
                     /label=BBa_B1001
     misc_feature    1997..2000
                     /label=conserved
     RBS             1993..2004
                     /label=BBa_B0034
     CDS             2011..2730
                     /label=GFP protein
     CDS             2011..2730
                     /label=BBa_E0040
     stem_loop       2750..2793
                     /label=stem_loop
     misc_feature    2739..2818
                     /label=BBa_B0010
     terminator      2739..2818
                     /label=BBa_B0010
     stem_loop       2834..2853
                     /label=T7 TE
     misc_feature    2860..2860
                     /label=stop
     polyA_site      2854..2867
                     /label=polya
     misc_feature    2827..2867
                     /label=BBa_B0012
     terminator      2827..2867
                     /label=BBa_B0012
     misc_feature    1951..2867
                     /label=BBa_B1201
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc
     1261 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa
     1321 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt
     1381 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt
     1441 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg
     1501 gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc
     1561 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac
     1621 ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa
     1681 cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa
     1741 gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag
     1801 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac
     1861 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc
     1921 tgatagtgct agtgtagatc gctactagag aaaaaaaaac cccgcttcgg cggggttttt
     1981 tttttactag agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga
     2041 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
     2101 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
     2161 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc
     2221 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa
     2281 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
     2341 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
     2401 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
     2461 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac
     2521 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
     2581 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
     2641 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
     2701 catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa
     2761 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     2821 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//