aspA

BBa_C0083 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_C0083
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
Date created: 2004-01-27 12:00:00
Date modified: 2015-08-31 04:07:24

aspartate ammonia-lyase



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_C0083_sequence (Version 1)

Description

Coding sequence for AspA enzyme. AspA aminates fumarate to make aspartate. Aspartate can be used as a bacterial chemotaxis signal.

Notes

The start codon was changed from GTG to ATG to match BioBricks standards.

Five silent mutations were introduced to eliminate EcoRI and PstI restriction enzyme sites. EcoRI sites were eliminated at bases 588 and 657 with C -> T mutations. PstI sites were eliminated at bases 624, 999, and 1125 with G -> A mutations. The mutations were designed to match codons with similar frequencies of usage in E. coli.

Source

AE000486 E. coli K12

Sequence Annotation Location Component / Role(s)
Help:Barcodes
AspA
G
C
G
C
G
G
LVA
1519,1543
1,1479
1,1
588,588
624,624
657,657
999,999
1125,1125
1480,1512
feature/barcode engineered_tag
feature/cds CDS
point_mutation feature/s_mutation
feature/s_mutation point_mutation
feature/s_mutation point_mutation
feature/s_mutation point_mutation
feature/s_mutation point_mutation
point_mutation feature/s_mutation
tag feature/tag
igem#experience
None
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_C0083/1