lacZ a

BBa_E0033 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_E0033
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yong-Su Jin (Fighting Darwins)
Date created: 2004-01-27 12:00:00
Date modified: 2015-08-31 04:07:25

LacZ alpha fragment
complements matching N-terminal deletion mutant (lacZ-omega)



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_E0033_sequence (Version 1)

Description

to greatly reduce the number of bases, this is only a portion of the LacZ gene

Notes

Restriction sites (modified)

76-79 (gcc to gca)
79-81 (gct to gca)
85-87 (gaa to gag)
106-108 (tgc to tgt)
109-111(agg to aga)

Source

www.ncbi.nlm.nih.gov

Sequence Annotation Location Component / Role(s)
T3 promoter
T7 promoter
lacZ gene fragment
lacZ gene fragment
lacZ alpha
T
A
C
C
G
26,35
173,191
199,348
1,15
1,348
81,81
87,87
108,108
78,78
111,111
promoter feature/promoter
feature/promoter promoter
sequence_feature feature/misc
sequence_feature feature/misc
CDS feature/cds
feature/mutation sequence_alteration
sequence_alteration feature/mutation
feature/mutation sequence_alteration
sequence_alteration feature/mutation
feature/mutation sequence_alteration
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_E0033/1