BBa_E0043 1 BBa_E0043 green fluorescent protein (GFP) variant 2008-02-24T12:00:00Z 2015-08-31T04:07:25Z This GFP variant was created via PCR of E0040. This is intended to be a "better" GFP variant over E0040(GFPmut3). It is is closer to EGFP and contains half of the amino acid mutations present in Emerald. See http://openwetware.org/wiki/GFP for more information about GFP. false false _41_ 0 22 84 Not in stock false 6 mutations relative to E0040 were inserted. These change the two amino acids at positions 64 and 65 (wild-type GFP numbering): F64L and S65T. An extra silent mutation in the previous amino acid 63 was introduced at the same time to optimize it slightly better for E. coli. false Austin Che annotation1961248 1 GFP range1961248 1 1 720 BBa_E0043_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z