BBa_E0051

BBa_E0051 Version 1

Component

Source: http://parts.igem.org/Part:BBa_E0051

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Austin Che

Date created: 2009-03-01 12:00:00

Date modified: 2015-08-31 04:07:25

lacZa.GFP fusion

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_E0051_sequence (Version 1)

Description

This part is a fusion protein between GFP and the alpha fragment of lacZ. It can be measured via fluorescence from GFP or through an assay for beta-galactosidase in a strain containing the omega fragment of lacZ.

Notes

The amino acid linker AGGSEGGGSEHHHHHHGSE is between the lacZa and GFP. The 6-his can also facilitate detection on a Western blot, for purification, etc.

Source

amino acids 3-60 from lacZ and amino acids 2-end of GFP (E0043)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
lacZa	1,180	CDS, feature/cds
linker	181,237	feature/misc, sequence_feature
GFP	238,954	feature/cds, CDS

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_E0051/1

Member of these Collections

• iGEM Parts Registry