LOCUS       BBa_E0601               3045 bp    DNA     linear       01-Jun-2024
DEFINITION  I0500.Q04400.E0432
ACCESSION   BBa_E0601
VERSION     BBa_E0601.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1240..1856
                     /label=tetR
     misc_feature    1857..1890
                     /label=SsrA
     misc_feature    1897..1921
                     /label=Help:Barcodes
     CDS             1237..1896
                     /label=BBa_C0040
     stem_loop       1941..1984
                     /label=stem_loop
     misc_feature    1930..2009
                     /label=BBa_B0010
     terminator      1930..2009
                     /label=BBa_B0010
     stem_loop       2025..2044
                     /label=T7 TE
     misc_feature    2051..2051
                     /label=stop
     polyA_site      2045..2058
                     /label=polya
     misc_feature    2018..2058
                     /label=BBa_B0012
     terminator      2018..2058
                     /label=BBa_B0012
     misc_feature    2067..2085
                     /label=TetR 1
     misc_feature    2067..2120
                     /label=BBa_R0040
     promoter        2086..2091
                     /label=-35
     misc_feature    2092..2110
                     /label=TetR 2
     promoter        2109..2114
                     /label=-10
     promoter        2067..2120
                     /label=BBa_R0040
     misc_feature    2133..2136
                     /label=conserved
     RBS             2129..2140
                     /label=BBa_B0034
     CDS             2147..2908
                     /label=YFP (LVA)
     misc_feature    2903..2908
                     /label=2
     misc_feature    2864..2902
                     /label=SsrA
     misc_feature    2388..2388
                     /label=A (Q->L)
     misc_feature    2147..2908
                     /label=BBa_E0032
     misc_feature    2147..2908
                     /label=BBa_E0032
     stem_loop       2928..2971
                     /label=stem_loop
     misc_feature    2917..2996
                     /label=BBa_B0010
     terminator      2917..2996
                     /label=BBa_B0010
     stem_loop       3012..3031
                     /label=T7 TE
     misc_feature    3038..3038
                     /label=stop
     polyA_site      3032..3045
                     /label=polya
     misc_feature    3005..3045
                     /label=BBa_B0012
     terminator      3005..3045
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatgt ccagattaga taaaagtaaa
     1261 gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt
     1321 aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag
     1381 cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc
     1441 cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt
     1501 gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa
     1561 cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag
     1621 aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat
     1681 caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca
     1741 ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc
     1801 ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgct
     1861 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca
     1921 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     1981 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     2041 ggcctttctg cgtttatata ctagagtccc tatcagtgat agagattgac atccctatca
     2101 gtgatagaga tactgagcac tactagagaa agaggagaaa tactagatgg tgagcaaggg
     2161 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     2221 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     2281 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt
     2341 cggctacggc ctgcaatgct tcgcccgcta ccccgaccac atgaagctgc acgacttctt
     2401 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     2461 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     2521 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     2581 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     2641 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     2701 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca
     2761 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     2821 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagaggcctg ctgcaaacga
     2881 cgaaaactac gctttagtag cttaataata ctagagccag gcatcaaata aaacgaaagg
     2941 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     3001 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//