mRFP1

BBa_E2050 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_E2050
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ryu
Date created: 2005-01-24 12:00:00
Date modified: 2016-01-25 02:35:54

derivative of mRFP1, yeast-optimized



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_E2050_sequence (Version 1)

Description

mRFP derivative. Ex548nm/Em562. yeast codon optimized.

Notes

Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2060 (mCherry), BBa_E2020 (Cerulean CFP), and BBa_E2030 (Venus YFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. except for 5' and 3' ends no significant sequence identity runs to mCherry (BBa_E2060).

Source

Shaner et al, 2004. Nat Biotech (22):1567-1571

Sequence Annotation Location Component / Role(s)
MATSG linker
GSGTA linker
mOrange
Help:Barcodes
1,15
724,738
1,738
745,769
sequence_feature feature/misc
sequence_feature feature/misc
feature/cds CDS
engineered_tag feature/barcode
igem#experience
None
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_E2050/1