LOCUS       BBa_I0404               1753 bp    DNA     linear       17-May-2024
DEFINITION  R0040.B0034.C0071.E0420
ACCESSION   BBa_I0404
VERSION     BBa_I0404.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..803
                     /label=rhlR
     misc_feature    804..836
                     /label=LVA
     misc_feature    843..867
                     /label=Help:Barcodes
     CDS             81..842
                     /label=BBa_C0071
     misc_feature    880..883
                     /label=conserved
     RBS             876..887
                     /label=BBa_B0034
     CDS             894..1616
                     /label=BBa_E0020
     stem_loop       1636..1679
                     /label=stem_loop
     misc_feature    1625..1704
                     /label=BBa_B0010
     terminator      1625..1704
                     /label=BBa_B0010
     stem_loop       1720..1739
                     /label=T7 TE
     misc_feature    1746..1746
                     /label=stop
     polyA_site      1740..1753
                     /label=polya
     misc_feature    1713..1753
                     /label=BBa_B0012
     terminator      1713..1753
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaggaatg acggaggctt tttgctgtgg tgggacggtt
      121 tgcgtagcga gatgcagccg atccacgaca gccagggcgt gttcgccgtc ctggaaaagg
      181 aagtgcggcg cctgggcttc gattactacg cctatggcgt gcgccacacg attcccttca
      241 cccggccgaa gaccgaggtc catggcacct atcccaaggc ctggctggag cgataccaga
      301 tgcagaacta cggggccgtg gatccggcga tcctcaacgg cctgcgctcc tcggaaatgg
      361 tggtctggag cgacagcctg ttcgaccaga gccggatgct ctggaacgag gctcgcgatt
      421 ggggcctctg tgtcggcgcg accttgccga tccgcgcgcc gaacaatttg ctcagcgtgc
      481 tttccgtggc gcgcgaccag cagaacatct ccagcttcga gcgcgaggaa atccgcctgc
      541 ggctgcgttg catgatcgag ttgctgaccc agaagctgac cgacctggag catccgatgc
      601 tgatgtccaa cccggtctgc ctgagccatc gcgaacgcga gatcctgcaa tggaccgccg
      661 acggcaagag ttccggggaa atcgccatca tcctgagcat ctccgagagc acggtgaact
      721 tccaccacaa gaacatccag aagaagttcg acgcgccgaa caagacgctg gctgccgcct
      781 acgccgcggc gctgggtctc atcgctgcaa acgacgaaaa ctacgcttta gtagcttaat
      841 aacactgata gtgctagtgt agatcactac tagagaaaga ggagaaatac tagatggtga
      901 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      961 tgaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     1021 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     1081 ccaccctgac ctggggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     1141 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     1201 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     1261 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     1321 agtacaacta catcagccac aacgtctata tcaccgccga caagcagaag aacggcatca
     1381 aggccaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     1441 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1501 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     1561 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taataatact
     1621 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1681 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1741 ttctgcgttt ata
//