LOCUS       BBa_I0405               1633 bp    DNA     linear       26-Apr-2024
DEFINITION  R0040.B0034.C0070.E0420
ACCESSION   BBa_I0405
VERSION     BBa_I0405.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..683
                     /label=rhlL
     misc_feature    684..716
                     /label=LVA
     misc_feature    723..747
                     /label=Help:Barcodes
     CDS             81..722
                     /label=BBa_C0070
     misc_feature    760..763
                     /label=conserved
     RBS             756..767
                     /label=BBa_B0034
     CDS             774..1496
                     /label=BBa_E0020
     stem_loop       1516..1559
                     /label=stem_loop
     misc_feature    1505..1584
                     /label=BBa_B0010
     terminator      1505..1584
                     /label=BBa_B0010
     stem_loop       1600..1619
                     /label=T7 TE
     misc_feature    1626..1626
                     /label=stop
     polyA_site      1620..1633
                     /label=polya
     misc_feature    1593..1633
                     /label=BBa_B0012
     terminator      1593..1633
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgatcgaac tgctgtccga atccctggaa ggtctgtccg
      121 ctgctatgat cgctgaactg ggtcgttacc gtcaccaggt tttcatcgaa aaactgggtt
      181 gggacgttgt ttccacctcc cgtgttcgtg accaggagtt cgaccagttc gaccacccgc
      241 agacccgtta catcgttgct atgtcccgtc agggtatctg cggttgcgct cgtctgctgc
      301 cgaccaccga cgcttacctg ctgaaagacg ttttcgctta cctgtgctcc gaaaccccgc
      361 cgtccgaccc gtccgtttgg gaactgtccc gttacgctgc ttccgctgct gacgacccgc
      421 agctggctat gaaaatcttc tggtcctccc tccagtgcgc ttggtacctg ggtgcttcct
      481 ccgttgttgc tgttaccacc accgctatgg aacgttactt cgttcgtaac ggtgttatcc
      541 tccagcgtct gggtccgccg cagaaagtta aaggtgaaac cctggttgct atctccttcc
      601 cggcttacca ggaacgtggt ctggaaatgc tgctgcgtta ccacccggaa tggctccagg
      661 gtgttccgct gtccatggct gttgctgcaa acgacgaaaa ctacgcttta gtagcttaat
      721 aactctgata gtgctagtgt agatctctac tagagaaaga ggagaaatac tagatggtga
      781 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      841 tgaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
      901 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      961 ccaccctgac ctggggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     1021 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     1081 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     1141 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     1201 agtacaacta catcagccac aacgtctata tcaccgccga caagcagaag aacggcatca
     1261 aggccaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     1321 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1381 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     1441 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taataatact
     1501 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1561 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1621 ttctgcgttt ata
//