LOCUS       BBa_I0413                943 bp    DNA     linear       07-May-2024
DEFINITION  RBS Test R0040.B0030.E0030.B0015
ACCESSION   BBa_I0413
VERSION     BBa_I0413.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS-1\Strong
     RBS             70..74
                     /label=RBS
     misc_feature    63..77
                     /label=BBa_B0030
     RBS             63..77
                     /label=BBa_B0030
     CDS             84..806
                     /label=BBa_E0030
     stem_loop       826..869
                     /label=stem_loop
     misc_feature    815..894
                     /label=BBa_B0010
     terminator      815..894
                     /label=BBa_B0010
     stem_loop       910..929
                     /label=T7 TE
     misc_feature    936..936
                     /label=stop
     polyA_site      930..943
                     /label=polya
     misc_feature    903..943
                     /label=BBa_B0012
     terminator      903..943
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaatac tagatggtga gcaagggcga ggagctgttc accggggtgg
      121 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
      181 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
      241 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg caatgcttcg
      301 cccgctaccc cgaccacatg aagctgcacg acttcttcaa gtccgccatg cccgaaggct
      361 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
      421 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
      481 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
      541 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
      601 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
      661 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca
      721 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
      781 gcatggacga gctgtacaag taataatact agagccaggc atcaaataaa acgaaaggct
      841 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      901 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//