BBa_I1012
1
BBa_I1012
CI(1) micRNA asRNA
2003-01-31T12:00:00Z
2015-08-31T04:07:29Z
Custom design.
Region which serves as basis for transcription of asRNA that binds to and inhibits <bb_part>BBa_I1010</bb_part>'s mRNA transcript via micRNA mechanism (See below for details). Has tetR promoter <bb_part>BBa_R0040</bb_part>. Part of the XOR gate comprised of <bb_part>BBa_I1010</bb_part> and <bb_part>BBa_I1020</bb_part>, their corresponding asRNA coding sequences (<bb_part>BBa_I1012</bb_part> and <bb_part>BBa_I1022</bb_part>), and the LacO-1 and TetR <bb_part>BBa_R0040</bb_part> promoters.
false
false
_1_
0
24
7
It's complicated
false
References (unparsed) here: <p>Mizuno, T., et al. <em>Proc. Natl. Acad. Sci. USA</em> (1984) 81, 1966-1970. <P>Coleman, J., et al. <em>Cell</em> (1984) 37, 429-36. <P> References (unparsed) here: <p>Mizuno, T., et al. <em>Proc. Natl. Acad. Sci. USA</em> (1984) 81, 1966-1970. <P>Coleman, J., et al. <em>Cell</em> (1984) 37, 429-36. <P>Complementary to beginning of <bb_part>BBa_I1010</bb_part> transcript covering RBS, start codon, and 73 bp into coding sequence. No designed secondary structure. Complementary region is flanked by two stem loop structures, as specified by the micRNA mechanism. <blockquote> <B>Anti-sense</B></P> <P>The success of this system clearly rests on the ability to effectively and specifically target mRNA transcripts for degradation using anti-sense RNA. While many papers, articles, and books have been written on the subject, there are no consensus anti-sense building strategies presented. We thus chose to implement three different types of antisense inhibition: KISS, micRNA, and IS10. In the description that follows, the following nomenclature will be used:</P> <P><em>target</em>- the mRNA transcript that we wish to inhibit.</P> <P><em>anti-sense</em>- the anti-sense molecule which will bind and inhibit <em>target</em>. </P> <blockquote> <P><em><strong>micRNA</strong></em></P> <img src="http://biobricks.ai.mit.edu/IAP_Projects/YoungPower/as_micRNA.gif"> <P>This anti-sense mechanism relies on two stem loops flanking an anti-sense sequence that is specific for the target. The function of the stem loops is to maintain the anti-sense region in a quasi-linear state. BBa_I1012 is built in this manner, with a linear region that will bind over the RBS, start codon, and 76 bp of BBa_I1010.</P> </blockquote></blockquote><P> Incompatible with systems containing <bb_part>BBa_I1011</bb_part>, <bb_part>BBa_I1013</bb_part>. <br>Compatible with <bb_part>BBa_I1020</bb_part>, <bb_part>BBa_I1021</bb_part>, <bb_part>BBa_I1022</bb_part>, <bb_part>BBa_I1023</bb_part>.
false
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1804
1
stem_loop
range1804
1
161
195
annotation7044
1
BBa_I1012
range7044
1
1
195
annotation1802
1
stem_loop
range1802
1
1
57
annotation1806
1
Reverse Complement RBS
range1806
1
138
143
annotation1809
1
start
range1809
1
131
133
annotation1807
1
reverse complement to cI cds
range1807
1
58
133
annotation1805
1
Reverse Complement to cI mRNA
range1805
1
58
160
BBa_I1012_sequence
1
taaaatcaataacttattcttaagtatttgacagcactgaatgtcaaaacaaaaccttttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtcgagcacatcttgttgtctgattattgatttttcgctatttcaaccggatgcctggcattcggtttttttt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z