LOCUS       BBa_I1060                835 bp    DNA     linear       17-May-2024
DEFINITION  Custom cI(2) Protein w/LacO-1 Promoter (Used for Anti-sense 
            inhibition)
ACCESSION   BBa_I1060
VERSION     BBa_I1060.1  
FEATURES             Location/Qualifiers
     stem_loop       56..85
                     /label=stem_loop
     RBS             73..78
                     /label=rbs
     misc_feature    83..85
                     /label=start
     CDS             83..835
                     /label=cI lambda
     promoter        21..26
                     /label=-35
     promoter        44..49
                     /label=-10
     misc_feature    56..158
                     /label=Complemenatry to Antisense mRNA I1063
     misc_feature    797..835
                     /label=SsrA
     misc_feature    830..835
                     /label=2
     misc_feature    1..20
                     /label=LacO-1 region
     misc_feature    27..43
                     /label=LacO-1
     misc_feature    56..56
                     /label=start
     misc_feature    58..58
                     /label=HH117a swap
     misc_feature    86..88
                     /label=C added
     misc_feature    89..90
                     /label=Binds BBa_I1063
     misc_feature    72..72
                     /label=swap
     misc_feature    79..79
                     /label=swap
     misc_feature    81..81
                     /label=swap
     misc_feature    82..82
                     /label=swap
     misc_feature    1..835
                     /label=BBa_I1060
ORIGIN
        1 ataaatgtga gcggataaca ttgacattgt gagcggataa caagatactg agcacgccaa
       61 aaatcaataa tgagacaaga tcatgtgcag tactaagaaa aagcctttga ctcaggaaca
      121 actcgaagat gcccgccgtc tcaaggccat ctacgagaaa aagaaaaatg aacttggctt
      181 atcccaggaa tctgtcgcag acaagatggg gatggggcag tcaggcgttg gtgctttatt
      241 taatggcatc aatgcattaa atgcttataa cgccgcattg cttgcaaaaa ttctcaaagt
      301 tagcgttgaa gaatttagcc cttcaatcgc cagagaaatc tacgagatgt atgaagcggt
      361 tagtatgcag ccgtcactta gaagtgagta tgagtaccct gttttttctc atgttcaggc
      421 agggatgttc tcacctgagc ttagaacctt taccaaaggt gatgcggaga gatgggtaag
      481 cacaaccaaa aaagccagtg attctgcatt ctggcttgag gttgaaggta attccatgac
      541 cgcaccaaca ggctccaagc caagctttcc tgacggaatg ttaattctcg ttgaccctga
      601 gcaggctgtt gagccaggtg atttctgcat agccagactt gggggtgatg agtttacctt
      661 caagaaactg atcagggata gcggtcaggt gtttttacaa ccactaaacc cacagtaccc
      721 aatgatccca tgcaatgaga gttgttccgt tgtggggaaa gttatcgcta gtcagtggcc
      781 tgaagagacg tttggcgctg caaacgacga aaactacgct ttagtagctt aataa
//