LOCUS       BBa_I11060              1871 bp    DNA     linear       17-May-2024
DEFINITION  Lambda half bit
ACCESSION   BBa_I11060
VERSION     BBa_I11060.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=spacer
     misc_feature    4..9
                     /label=KpnI
     misc_feature    10..12
                     /label=spacer
     RBS             13..18
                     /label=RBS (BBa_I11010)
     misc_feature    19..23
                     /label=spacer
     CDS             24..1091
                     /label=Integrase lambda
     misc_feature    1092..1124
                     /label=LVA
     misc_feature    1125..1130
                     /label=stop
     misc_feature    1131..1133
                     /label=spacer
     misc_feature    1134..1139
                     /label=AclI
     misc_feature    1140..1142
                     /label=spacer
     misc_feature    1143..1402
                     /label=P22 attP (BBa_I11033)
     misc_feature    1403..1405
                     /label=spacer
     misc_feature    1406..1411
                     /label=PvuI
     misc_feature    1412..1414
                     /label=spacer
     stem_loop       1415..1544
                     /label=ReverseTerminator (BBa_B0025)
     misc_feature    1545..1547
                     /label=spacer
     misc_feature    1548..1553
                     /label=XmaI
     misc_feature    1554..1556
                     /label=spacer
     misc_feature    1557..1583
                     /label=P22 attB rev comp (BBa_I11032)
     misc_feature    1584..1586
                     /label=spacer
     misc_feature    1587..1592
                     /label=BmtI
     RBS             1593..1598
                     /label=RBS (BBa_I11010)
     misc_feature    1599..1603
                     /label=spacer
     CDS             1604..1819
                     /label=Xis lambda
     misc_feature    1820..1852
                     /label=AAV
     misc_feature    1853..1858
                     /label=stop
     misc_feature    1859..1861
                     /label=spacer
     misc_feature    1862..1869
                     /label=NotI
     misc_feature    1870..1871
                     /label=spacer
ORIGIN
        1 atcggtaccg caaggaggtc atcatgggaa gaaggcgaag tcatgagcgc cgggatttac
       61 cccctaacct ttatataaga aacaatggat attactgcta cagggaccca aggacgggta
      121 aagagtttgg attaggcaga gacaggcgaa tcgcaatcac tgaagctata caggccaaca
      181 ttgagttatt ttcaggacac aaacacaagc ctctgacagc gagaatcaac agtgataatt
      241 ccgttacgtt acattcatgg cttgatcgct acgaaaaaat cctggccagc agaggaatca
      301 agcagaagac actcataaat tacatgagca aaattaaagc aataaggagg ggtctgcctg
      361 atgctccact tgaagacatc accacaaaag aaattgcggc aatgctcaat ggatacatag
      421 acgagggcaa ggcggcgtca gccaagttaa tcagatcaac actgagcgat gcattccgag
      481 aggcaatagc tgaaggccat ataacaacaa accatgtcgc tgccactcgc gcagcaaaat
      541 cagaggtaag gagatcaaga cttacggctg acgaatacct gaaaatttat caagcagcag
      601 aatcatcacc atgttggctc agacttgcaa tggaactggc tgttgttacc gggcaacgag
      661 ttggtgattt atgcgaaatg aagtggtctg atatcgtaga tggatatctt tatgtcgagc
      721 aaagcaaaac aggcgtaaaa attgccatcc caacagcatt gcatattgat gctctcggaa
      781 tatcaatgaa ggaaacactt gataaatgca aagagattct tggcggagaa accataattg
      841 catctactcg tcgcgaaccg ctttcatccg gcacagtatc aaggtatttt atgcgcgcac
      901 gaaaagcatc aggtctttcc ttcgaagggg acccgcctac ctttcacgag ttgcgcagtt
      961 tgtctgcaag actctatgag aagcagataa gcgataagtt tgctcaacat cttctcgggc
     1021 ataagtcgga caccatggca tcacagtatc gtgatgacag aggcagggag tgggacaaaa
     1081 ttgaaatcaa agcagcaaac gacgaaaact acgctttagt agcttaataa gcaaacgttc
     1141 gactaagtgg tttgggacaa aaatgggaca tacaaatctt tgcatcggtt tgcaaggctt
     1201 tgcatgtctt tcgaagatgg gacgtgtgag cgcaggtatg acgtggtatg ttgttgactt
     1261 aaaaggtagt tcttataatt cgtaatgcga aggtcgtagg ttcgactcct attatcggca
     1321 ccagttaaat caaatactta cgtattattc gtgccttcct tatttttact gtgggacata
     1381 tttgggacag aagtaccaaa aaacacgatc ggtatataaa cgcagaaagg cccacccgaa
     1441 ggtgagccag tgtgactcta agtagagagc gttcaccgac aaacaacaga taaaacgaaa
     1501 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggattccc gggtcaacga
     1561 ccttcgcatt acgaatgcgc tgccttgcta gcaggaggct accatgtact tgacacttca
     1621 ggagtggaac gcacgccagc gacgtccaag aagccttgaa acagttcgtc gatgggttcg
     1681 ggaatgcagg atattcccac ctccggttaa ggatggaaga gagtatctgt tccacgaatc
     1741 agcggtaaag gttgacttaa atcgaccagt aacaggtggc cttttgaaga ggatcagaaa
     1801 tgggaagaag gcgaagtcag cagcaaacga cgaaaactac gctgctgctg tttaataata
     1861 cgcggccgcc t
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