Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: mcnamara
Date created: 2004-07-13 11:00:00
Date modified: 2015-08-31 04:07:31
Modified lamdba Prm promoter (repressed by 434 cI)
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_I12006_sequence (Version 1) |
Description
Lamdba Prm promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI)Notes
The O-R1 region of 434 contained 14 base pairs as opposed to the 17 base pairs of the O-R3 site of lambda. Also, it was noticed that the O-R3 site of the lambda included part of the -10 site. Hence, to preserve the spacing and the -10 site, the three nucleotides that were in both the -10 site and the lambda O-R3 site were retained. The 14 nucleotides that were in the O-R3 site and not in the -10 site were replaced with the O-R1 site of the 434.Source
Bushman(1993), Shih & Gussin (1983)| Sequence Annotation | Location | Component / Role(s) |
| OR1 434 OR1 lambda OR2 lambda -10 -35 | 56,69 9,25 33,49 71,76 48,53 | feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site promoter feature/promoter promoter feature/promoter |