Prm +

BBa_I12007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I12007
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hans
Date created: 2004-07-14 11:00:00
Date modified: 2015-08-31 04:07:31

Modified lambda Prm promoter (OR-3 obliterated)



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_I12007_sequence (Version 1)

Description

Lambda Prm promoter modified to be activated but not repressed by the lambda repressor (cI)

Notes

In wild type lambda phage, the OR3 site (-27 to -11) reads "tatcccttgcggtgata" on the sense strand of DNA. To prevent lambda repressor (cI) from binding to this site, the 4th through 10th nt of OR3 were replaced by the 7 nt between OR2 and OR1, "aaatagt" (-57 to -51), which in effect mutates 5 nt of the promoter. These nt were chosen to be about halfway between the -10 and -35 boxes of the promoter. Furthermore, since these nt already act as a spacer in wild-type phage, it is hoped they will not create undesired interactions.

Source

Shih and Gussin (1983)

Sequence Annotation Location Component / Role(s)
OR1
OR2
-35
mutate to obliterate OR3
-10
9,25
33,49
48,53
59,65
71,76
feature/binding non_covalent_binding_site
non_covalent_binding_site feature/binding
feature/promoter promoter
feature/mutation sequence_alteration
promoter feature/promoter
igem#experience
None
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_I12007/1