LOCUS       BBa_I12011              1094 bp    DNA     linear       11-May-2024
DEFINITION  Barkai-Leibler design experiment part B (p22cII)
ACCESSION   BBa_I12011
VERSION     BBa_I12011.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     misc_feature    146..162
                     /label=OR1
     misc_feature    170..186
                     /label=OR2
     promoter        185..190
                     /label=-35
     misc_feature    196..202
                     /label=mutate to obliterate OR3
     promoter        208..213
                     /label=-10
     promoter        138..219
                     /label=BBa_I12007
     misc_feature    232..235
                     /label=conserved
     RBS             228..239
                     /label=BBa_B0034
     CDS             246..893
                     /label=cII p22
     misc_feature    894..926
                     /label=LVA
     misc_feature    927..932
                     /label=stop
     misc_feature    246..932
                     /label=BBa_C0053
     misc_feature    933..957
                     /label=Help:Barcodes
     CDS             246..932
                     /label=BBa_C0053
     stem_loop       977..1020
                     /label=stem_loop
     misc_feature    966..1045
                     /label=BBa_B0010
     terminator      966..1045
                     /label=BBa_B0010
     stem_loop       1061..1080
                     /label=T7 TE
     misc_feature    1087..1087
                     /label=stop
     polyA_site      1081..1094
                     /label=polya
     misc_feature    1054..1094
                     /label=BBa_B0012
     terminator      1054..1094
                     /label=BBa_B0012
     terminator      966..1094
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggca accattatca ccgccagagg taaaatagtc aacacgcacg
      181 gtgttagata tttataaata gtggtgatag atttaacgtt actagagaaa gaggagaaat
      241 actagatgaa tacacaattg atgggtgagc gtattcgcgc tcgaagaaaa aaactcaaga
      301 ttagacaagc cgctcttggt aagatggtgg gagtgtctaa tgttgcaata tcgcaatggg
      361 agcgctcgga gactgagcca aatggggaga acctgttggc actttcgaag gctcttcagt
      421 gctcccctga ctatttgctg aaaggagatt taagccagac aaacgttgcc tatcatagta
      481 ggcatgagcc aagaggatca taccctctta tcagttgggt aagcgcaggg caatggatgg
      541 aagctgtaga accttatcac aagcgcgcga tagagaactg gcacgacacc actgtagatt
      601 gttcagaaga ttcattttgg cttgatgtcc aaggtgactc tatgacagca ccggcagggt
      661 taagcattcc agaaggaatg ataattctgg ttgatcccga agtcgaacca agaaacggca
      721 agctggttgt tgcaaaatta gaaggtgaaa acgaggccac attcaaaaaa ttagttatgg
      781 atgcaggccg aaagttttta aaaccattaa acccacaata tccgatgata gaaatcaacg
      841 gaaactgcaa aatcattggc gtagttgttg acgcaaaact cgcaaatctt ccagctgcaa
      901 acgacgaaaa ctacgcttta gtagcttaat aacactgata gtgctagtgt agatcactac
      961 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     1021 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     1081 tttctgcgtt tata
//